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The map label for this gene is ispG

Identifier: 85374900

GI number: 85374900

Start: 2103232

End: 2104374

Strand: Direct

Name: ispG

Synonym: ELI_10365

Alternate gene names: 85374900

Gene position: 2103232-2104374 (Clockwise)

Preceding gene: 85374898

Following gene: 85374901

Centisome position: 68.9

GC content: 64.57

Gene sequence:

>1143_bases
ATGTCCTCCGTAAGACCTTGGCGCGATATCGAGCGTCGCGAGTGCCGCCAGATCATGGTCGGCGATGTGCCCGTGGGCGG
CGGCGCGCCGATCACGGTGCAGACCATGACCAACACGCCGACCGAAGACGCCGTCGCCACCATCGACCAGATCCGCCGCT
GCGAGGATGCCGGCGCGGACATCATTCGCGTTTCTGTGCCGACGGTCGAGGCCAGCCAGGCGTTCGGCAAGATCACCAAG
GCGGCGCGCGTACCGATCGTCGCCGACATCCATTTCCACTACAAGCGCGCGCTCGAAGCCGCCGATGCCGGTGCTGCCTG
CCTCCGCATCAACCCCGGCAATATCGGCAGCAGCGAGCGCGTGGCCGAAGTCGTTCGGGCCGCGAAAGCCAATGGCTGCG
CGATACGCATCGGCGTAAATGCGGGCAGCCTCGAGAAAGACCTGCTGGAGAAATACGGCGAGCCCTGCCCCGAGGCGCTC
ATCGAAAGCGCGCTCGACCATATCAAGCTGCTGCAGGACCACGATTTTCACGAATACAAAGTGGCGGTGAAGGCCTCCGA
CGTGTTTCTCGCGGTCGCCGCCTACCATGGGCTGGCCGAGGCCGTGGACTGCCCTTTGCACTTGGGCATCACCGAGGCTG
GCGGACTGATCGGTGGTACGGTCAAATCTTCCATCGGCATGGGCTCGCTGCTGTGGGCCGGGATCGGCGACACGATACGC
GTGTCGCTCAGCGCCGAGCCGGAGCAGGAAGTGAAGGTCGGCTACGAAATGCTCAAGGCGCTCGGCCTGCGGACGCGCGG
CGTGCGCGTCGTCTCCTGCCCCAGCTGTTCGCGCCAAGGGTTCGATGTGATCCGCACGGTCGAAGCGCTGGAAAAGCGGC
TCGAACACATCAAGACGCCGATGAGCCTCTCCGTCCTCGGCTGCGTCGTCAACGGCCCCGGCGAAGCGCGCGAGACCGAT
ATCGGCATCACCGGCGGCGGCAAGGGCAAGCACATGGTTTACCTCTCCGGCGTGACCGACCATCATGTCGAGGACACCGA
TATGCTCGACCACATCGTCAAGCTGGTCGAGGAGAAAGCCGCCAGGATCGAGGCCGGCGATGATATCGCTTTCGATCCGC
ATCAAGCCGAAGCTGCCGAGTGA

Upstream 100 bases:

>100_bases
TCGGCAAGGCGTTTTTCAGGCATCGCACCCTTTCAACGCGCAATTGCGAGCATGCGCAAGCGCCTTTAACCTGTCGCAGT
TCGTCCCTATAGGATGGCGC

Downstream 100 bases:

>100_bases
AGCGCGGAGCGATTTTCGCGGCGGCGCTGGCGCTGACATCCGTCTCAGTCACGGTTTCGGCGGAAGAGGGCGACACCTCG
GATCATCCCGAAGCACGGCT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSSVRPWRDIERRECRQIMVGDVPVGGGAPITVQTMTNTPTEDAVATIDQIRRCEDAGADIIRVSVPTVEASQAFGKITK
AARVPIVADIHFHYKRALEAADAGAACLRINPGNIGSSERVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEAL
IESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYHGLAEAVDCPLHLGITEAGGLIGGTVKSSIGMGSLLWAGIGDTIR
VSLSAEPEQEVKVGYEMLKALGLRTRGVRVVSCPSCSRQGFDVIRTVEALEKRLEHIKTPMSLSVLGCVVNGPGEARETD
IGITGGGKGKHMVYLSGVTDHHVEDTDMLDHIVKLVEEKAARIEAGDDIAFDPHQAEAAE

Sequences:

>Translated_380_residues
MSSVRPWRDIERRECRQIMVGDVPVGGGAPITVQTMTNTPTEDAVATIDQIRRCEDAGADIIRVSVPTVEASQAFGKITK
AARVPIVADIHFHYKRALEAADAGAACLRINPGNIGSSERVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEAL
IESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYHGLAEAVDCPLHLGITEAGGLIGGTVKSSIGMGSLLWAGIGDTIR
VSLSAEPEQEVKVGYEMLKALGLRTRGVRVVSCPSCSRQGFDVIRTVEALEKRLEHIKTPMSLSVLGCVVNGPGEARETD
IGITGGGKGKHMVYLSGVTDHHVEDTDMLDHIVKLVEEKAARIEAGDDIAFDPHQAEAAE
>Mature_379_residues
SSVRPWRDIERRECRQIMVGDVPVGGGAPITVQTMTNTPTEDAVATIDQIRRCEDAGADIIRVSVPTVEASQAFGKITKA
ARVPIVADIHFHYKRALEAADAGAACLRINPGNIGSSERVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALI
ESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYHGLAEAVDCPLHLGITEAGGLIGGTVKSSIGMGSLLWAGIGDTIRV
SLSAEPEQEVKVGYEMLKALGLRTRGVRVVSCPSCSRQGFDVIRTVEALEKRLEHIKTPMSLSVLGCVVNGPGEARETDI
GITGGGKGKHMVYLSGVTDHHVEDTDMLDHIVKLVEEKAARIEAGDDIAFDPHQAEAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=368, Percent_Identity=55.7065217391304, Blast_Score=413, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR011060 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40536; Mature: 40405

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVRPWRDIERRECRQIMVGDVPVGGGAPITVQTMTNTPTEDAVATIDQIRRCEDAGAD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
IIRVSVPTVEASQAFGKITKAARVPIVADIHFHYKRALEAADAGAACLRINPGNIGSSER
EEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
VAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALIESALDHIKLLQDHDFHEYK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAVKASDVFLAVAAYHGLAEAVDCPLHLGITEAGGLIGGTVKSSIGMGSLLWAGIGDTIR
EEEEECHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHHCCCCHHHHHCCCCEEE
VSLSAEPEQEVKVGYEMLKALGLRTRGVRVVSCPSCSRQGFDVIRTVEALEKRLEHIKTP
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
MSLSVLGCVVNGPGEARETDIGITGGGKGKHMVYLSGVTDHHVEDTDMLDHIVKLVEEKA
CCEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ARIEAGDDIAFDPHQAEAAE
HHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSVRPWRDIERRECRQIMVGDVPVGGGAPITVQTMTNTPTEDAVATIDQIRRCEDAGAD
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
IIRVSVPTVEASQAFGKITKAARVPIVADIHFHYKRALEAADAGAACLRINPGNIGSSER
EEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
VAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALIESALDHIKLLQDHDFHEYK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAVKASDVFLAVAAYHGLAEAVDCPLHLGITEAGGLIGGTVKSSIGMGSLLWAGIGDTIR
EEEEECHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHHCCCCHHHHHCCCCEEE
VSLSAEPEQEVKVGYEMLKALGLRTRGVRVVSCPSCSRQGFDVIRTVEALEKRLEHIKTP
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
MSLSVLGCVVNGPGEARETDIGITGGGKGKHMVYLSGVTDHHVEDTDMLDHIVKLVEEKA
CCEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ARIEAGDDIAFDPHQAEAAE
HHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA