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The map label for this gene is mmsB [H]

Identifier: 85374836

GI number: 85374836

Start: 2036950

End: 2037819

Strand: Direct

Name: mmsB [H]

Synonym: ELI_10045

Alternate gene names: 85374836

Gene position: 2036950-2037819 (Clockwise)

Preceding gene: 85374835

Following gene: 85374837

Centisome position: 66.73

GC content: 62.87

Gene sequence:

>870_bases
ATGAGAATCGCATTTATCGGCCTCGGCAATATGGGCGGCGGGATGGCCGCGAACCTCGTCAAGGCGGGGCACGATGTGCA
GGCCTTCGACCTCAGCCAGGCCGCGCTCGATACGGCGAAAGACAATGGCTGCACGCCCTTCACCTCGATCAAGGACACAT
GCGAAGGCGCAGAGGCGGTCGTCACGATGCTGCCCAATGGCGAGATCGTCCGGCAGGTCTACGTCGACGATGTCATCGGC
AGTGCGCCAAAAGGTGCCGTGCTGATCGACTGTTCGACGATCGACGTCGCAACGGCGCGGCAGGTCGAAGCGATTGCGGT
TGGCGCGGGTTACGAGATGGTCGATGCGCCGGTCTCCGGCGGGATCGCGGCGGCCAACGGCGGCACGCTGACATTCATGG
TCGGCGGGACCGAGAGCGCCTTCGGCCGCGCCAAGCTGATCCTCGAAGCCATGGGCAAGGCCGTGATCCACGCCGGCGAC
GCAGGCAACGGCCAGGCGGCCAAGATTTGCAACAACATGCTGCTGGCGATCCACATGATCGGCACCTGCGAAGCATTCAG
GATGGCCGAGAAGCTCGGCCTCGACCCCCAGACATTCTACGACATCTCTTCTGTCAGCTCGGGCCAGAACTGGTCGATGA
CGAGCTATTGCCCTGTCCCCGGCGTCGGCCCGCAAAGCCCCTCCGATAATGATTACCAGGGCGGTTTTGCCACTGCGCTG
ATGCTGAAGGATTTGCGTCTGGCGATGGAAGCCGCGGAAACGGCGGATGCGGCCACGCCGATGGGCGCACGTGCGGCGTC
GATCTATGAAGATTTCAACGGGGCAGGCAACGGCGGACTGGATTTCAGCGCCATCATCAAGATGCTTTAG

Upstream 100 bases:

>100_bases
CGGGCTTCCTCTTTCCATACCTCACCTACCTGATCTGGAAGCATGACGGAAACGCTGCGGCGATCATGCTTGGCGTCGAC
TACCCCGGGAAGGACACATC

Downstream 100 bases:

>100_bases
CAATCGCATCCAGGTGCATCACGACCACAACGCACTCGTTTTTTACTGGACCCGAAGGGCACGAGGCAGCCGCGATGCGG
TTTCGGTCGTAACAAAACTG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRIAFIGLGNMGGGMAANLVKAGHDVQAFDLSQAALDTAKDNGCTPFTSIKDTCEGAEAVVTMLPNGEIVRQVYVDDVIG
SAPKGAVLIDCSTIDVATARQVEAIAVGAGYEMVDAPVSGGIAAANGGTLTFMVGGTESAFGRAKLILEAMGKAVIHAGD
AGNGQAAKICNNMLLAIHMIGTCEAFRMAEKLGLDPQTFYDISSVSSGQNWSMTSYCPVPGVGPQSPSDNDYQGGFATAL
MLKDLRLAMEAAETADAATPMGARAASIYEDFNGAGNGGLDFSAIIKML

Sequences:

>Translated_289_residues
MRIAFIGLGNMGGGMAANLVKAGHDVQAFDLSQAALDTAKDNGCTPFTSIKDTCEGAEAVVTMLPNGEIVRQVYVDDVIG
SAPKGAVLIDCSTIDVATARQVEAIAVGAGYEMVDAPVSGGIAAANGGTLTFMVGGTESAFGRAKLILEAMGKAVIHAGD
AGNGQAAKICNNMLLAIHMIGTCEAFRMAEKLGLDPQTFYDISSVSSGQNWSMTSYCPVPGVGPQSPSDNDYQGGFATAL
MLKDLRLAMEAAETADAATPMGARAASIYEDFNGAGNGGLDFSAIIKML
>Mature_289_residues
MRIAFIGLGNMGGGMAANLVKAGHDVQAFDLSQAALDTAKDNGCTPFTSIKDTCEGAEAVVTMLPNGEIVRQVYVDDVIG
SAPKGAVLIDCSTIDVATARQVEAIAVGAGYEMVDAPVSGGIAAANGGTLTFMVGGTESAFGRAKLILEAMGKAVIHAGD
AGNGQAAKICNNMLLAIHMIGTCEAFRMAEKLGLDPQTFYDISSVSSGQNWSMTSYCPVPGVGPQSPSDNDYQGGFATAL
MLKDLRLAMEAAETADAATPMGARAASIYEDFNGAGNGGLDFSAIIKML

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=43.9446366782007, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI40556376, Length=292, Percent_Identity=27.0547945205479, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=31.7073170731707, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=34.6020761245675, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786719, Length=298, Percent_Identity=32.5503355704698, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789092, Length=293, Percent_Identity=29.3515358361775, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=42.0074349442379, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=42.3611111111111, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=42.3611111111111, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24655240, Length=64, Percent_Identity=45.3125, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29592; Mature: 29592

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFIGLGNMGGGMAANLVKAGHDVQAFDLSQAALDTAKDNGCTPFTSIKDTCEGAEAV
CEEEEEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHCCCEEE
VTMLPNGEIVRQVYVDDVIGSAPKGAVLIDCSTIDVATARQVEAIAVGAGYEMVDAPVSG
EEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCEEEEEEECCCCEECCCCCC
GIAAANGGTLTFMVGGTESAFGRAKLILEAMGKAVIHAGDAGNGQAAKICNNMLLAIHMI
CEEECCCCEEEEEECCCCCHHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHEEHHHH
GTCEAFRMAEKLGLDPQTFYDISSVSSGQNWSMTSYCPVPGVGPQSPSDNDYQGGFATAL
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHH
MLKDLRLAMEAAETADAATPMGARAASIYEDFNGAGNGGLDFSAIIKML
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRIAFIGLGNMGGGMAANLVKAGHDVQAFDLSQAALDTAKDNGCTPFTSIKDTCEGAEAV
CEEEEEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHCCCEEE
VTMLPNGEIVRQVYVDDVIGSAPKGAVLIDCSTIDVATARQVEAIAVGAGYEMVDAPVSG
EEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCEEEEEEECCCCEECCCCCC
GIAAANGGTLTFMVGGTESAFGRAKLILEAMGKAVIHAGDAGNGQAAKICNNMLLAIHMI
CEEECCCCEEEEEECCCCCHHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHEEHHHH
GTCEAFRMAEKLGLDPQTFYDISSVSSGQNWSMTSYCPVPGVGPQSPSDNDYQGGFATAL
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHH
MLKDLRLAMEAAETADAATPMGARAASIYEDFNGAGNGGLDFSAIIKML
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339433; 10984043 [H]