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The map label for this gene is bcr [H]

Identifier: 85374809

GI number: 85374809

Start: 2014031

End: 2015296

Strand: Reverse

Name: bcr [H]

Synonym: ELI_09910

Alternate gene names: 85374809

Gene position: 2015296-2014031 (Counterclockwise)

Preceding gene: 85374811

Following gene: 85374802

Centisome position: 66.02

GC content: 66.11

Gene sequence:

>1266_bases
ATGGCCGCAACCGACCCGAAAACCCGATCCGGCACTCCGCCGCTGGTCGAACGCGCGCCACAGCGCGCGCTGGGCGAGCG
CGAATTGATCGTGCTGATGGCGCTCCTGATGAGCCTGCAGGCGTTCGGGATCGATTCCATGCTGCCCGCGCTCGGCGTCA
TGGCGCAGGAACTCGTCGCGACCGGCAACGACCGGCAATTGGTGATCGGCGCCTATTTCCTCGGCGCAGGGATCGGCGCG
TTTTTCCCCGGAGCTTTCGCGGACCGTTTCGGGCGGCGACCGATCCTGTTCCTCGGCCTGCTGTCCTATATCGTGCTGTC
GCTCGCCTGCGCGCTGGTAACCGATTTCGAGACCATCGTCGCGCTGCGCTTCATCCAGGGGATCACCTGCGCGGGGCTCA
GCGTCGTCCCAGCCGCCATCGTGCGCGATCATGTCGGCGGCGATCGCATGGCGCGGATGATGAGCCTGATCATGATGATT
TTCCTGCTGGTGCCGATCCTCGCGCCCGCGATCGGGCAGGGCATCATGATGGTCGCTGGCTGGCGCGCGATCTTCGGCGC
AATGGCAGTGATGGGGCTGGGCGTCGGTGCCTGGGTGTGGTGGCGCCTACCCGAAACGCTGGCAGCGGAGAACAAGCAGG
AGATCGATCCGCGCACGATCCTGCGCAACATGTGGACCGCGCTCAGCACGCGCGGTGCGATCGGATATGTCGTCGGCAGC
GCGCTGGTGTTCGGATCGCTGTTCGGCTTCCTCAATTCGAGCCAGCAGCTGATCGGCGAGACTTTCGGCGCGAACGAATA
TTTCGCGCTGATCTTCGGGGCCGCCGTCGTGGGCATGGTGGTGGCGAACTTCACCAATTCGCGCATCGTCGAGCGTTTCG
GCGCGCGGCGGGTCGGCCATACCGCCCTGCTGGCCTTCATCCTCATCAGCGCTCTGCAAGTGTGGAGCGCGAGCCAGGCG
CCGCAAAGCCTGCTGGAATTTATCGTGCTGATGAGCCTGGCCATGGCGACGCTGGGTTTCGTCGGCGCGAACTTCAGCTC
GATTGCGATGCAGCCGTTCTTCCATATCGCCGGGGCCGCGAGCAGCGGGCAGACGGCGGTGCGGATGAGCACCGGCGCGA
TCCTCGGCAGCAGCATCGGCTATCTGTTCGACGGGACCGCAGTGCCGCTGGCAAGCGCGATGCTGAGCTGCGGCTTGCTG
GCCCTGGCGGCGGTCCTGTTCAGCGAGCGTGGCAGGCTCTTCGGGCGGCGGGTGGAGGGGCCCTAG

Upstream 100 bases:

>100_bases
CGATATCGTCCTCGGCCATCGCTGCCGCCATAGCGCGGCTTGGCCCGCATGTTCAGAAAAATGGCTGGCAAAGTGCCCGG
TCGCAGCGCAAGGGACCTGC

Downstream 100 bases:

>100_bases
GCCTGAGTCCGTCATCCCAGCGAACGCTGGGATCTGTCTCCTCCAAGTCGAGCGAAACTGACTGAGATCCCAGCTTTCGC
TGGGATCACGCTATGAAGCC

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAATDPKTRSGTPPLVERAPQRALGERELIVLMALLMSLQAFGIDSMLPALGVMAQELVATGNDRQLVIGAYFLGAGIGA
FFPGAFADRFGRRPILFLGLLSYIVLSLACALVTDFETIVALRFIQGITCAGLSVVPAAIVRDHVGGDRMARMMSLIMMI
FLLVPILAPAIGQGIMMVAGWRAIFGAMAVMGLGVGAWVWWRLPETLAAENKQEIDPRTILRNMWTALSTRGAIGYVVGS
ALVFGSLFGFLNSSQQLIGETFGANEYFALIFGAAVVGMVVANFTNSRIVERFGARRVGHTALLAFILISALQVWSASQA
PQSLLEFIVLMSLAMATLGFVGANFSSIAMQPFFHIAGAASSGQTAVRMSTGAILGSSIGYLFDGTAVPLASAMLSCGLL
ALAAVLFSERGRLFGRRVEGP

Sequences:

>Translated_421_residues
MAATDPKTRSGTPPLVERAPQRALGERELIVLMALLMSLQAFGIDSMLPALGVMAQELVATGNDRQLVIGAYFLGAGIGA
FFPGAFADRFGRRPILFLGLLSYIVLSLACALVTDFETIVALRFIQGITCAGLSVVPAAIVRDHVGGDRMARMMSLIMMI
FLLVPILAPAIGQGIMMVAGWRAIFGAMAVMGLGVGAWVWWRLPETLAAENKQEIDPRTILRNMWTALSTRGAIGYVVGS
ALVFGSLFGFLNSSQQLIGETFGANEYFALIFGAAVVGMVVANFTNSRIVERFGARRVGHTALLAFILISALQVWSASQA
PQSLLEFIVLMSLAMATLGFVGANFSSIAMQPFFHIAGAASSGQTAVRMSTGAILGSSIGYLFDGTAVPLASAMLSCGLL
ALAAVLFSERGRLFGRRVEGP
>Mature_420_residues
AATDPKTRSGTPPLVERAPQRALGERELIVLMALLMSLQAFGIDSMLPALGVMAQELVATGNDRQLVIGAYFLGAGIGAF
FPGAFADRFGRRPILFLGLLSYIVLSLACALVTDFETIVALRFIQGITCAGLSVVPAAIVRDHVGGDRMARMMSLIMMIF
LLVPILAPAIGQGIMMVAGWRAIFGAMAVMGLGVGAWVWWRLPETLAAENKQEIDPRTILRNMWTALSTRGAIGYVVGSA
LVFGSLFGFLNSSQQLIGETFGANEYFALIFGAAVVGMVVANFTNSRIVERFGARRVGHTALLAFILISALQVWSASQAP
QSLLEFIVLMSLAMATLGFVGANFSSIAMQPFFHIAGAASSGQTAVRMSTGAILGSSIGYLFDGTAVPLASAMLSCGLLA
LAAVLFSERGRLFGRRVEGP

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=392, Percent_Identity=27.0408163265306, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790146, Length=396, Percent_Identity=29.040404040404, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI48994889, Length=344, Percent_Identity=22.6744186046512, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082312, Length=328, Percent_Identity=27.7439024390244, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787065, Length=237, Percent_Identity=27.4261603375527, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790794, Length=151, Percent_Identity=28.476821192053, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44596; Mature: 44465

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATDPKTRSGTPPLVERAPQRALGERELIVLMALLMSLQAFGIDSMLPALGVMAQELVA
CCCCCCCCCCCCCHHHHHCHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGNDRQLVIGAYFLGAGIGAFFPGAFADRFGRRPILFLGLLSYIVLSLACALVTDFETIV
CCCCCEEEEHHHHHHCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRFIQGITCAGLSVVPAAIVRDHVGGDRMARMMSLIMMIFLLVPILAPAIGQGIMMVAG
HHHHHHCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WRAIFGAMAVMGLGVGAWVWWRLPETLAAENKQEIDPRTILRNMWTALSTRGAIGYVVGS
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ALVFGSLFGFLNSSQQLIGETFGANEYFALIFGAAVVGMVVANFTNSRIVERFGARRVGH
HHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
TALLAFILISALQVWSASQAPQSLLEFIVLMSLAMATLGFVGANFSSIAMQPFFHIAGAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SSGQTAVRMSTGAILGSSIGYLFDGTAVPLASAMLSCGLLALAAVLFSERGRLFGRRVEG
CCCCEEEEEEHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
P
C
>Mature Secondary Structure 
AATDPKTRSGTPPLVERAPQRALGERELIVLMALLMSLQAFGIDSMLPALGVMAQELVA
CCCCCCCCCCCCHHHHHCHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGNDRQLVIGAYFLGAGIGAFFPGAFADRFGRRPILFLGLLSYIVLSLACALVTDFETIV
CCCCCEEEEHHHHHHCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRFIQGITCAGLSVVPAAIVRDHVGGDRMARMMSLIMMIFLLVPILAPAIGQGIMMVAG
HHHHHHCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WRAIFGAMAVMGLGVGAWVWWRLPETLAAENKQEIDPRTILRNMWTALSTRGAIGYVVGS
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ALVFGSLFGFLNSSQQLIGETFGANEYFALIFGAAVVGMVVANFTNSRIVERFGARRVGH
HHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
TALLAFILISALQVWSASQAPQSLLEFIVLMSLAMATLGFVGANFSSIAMQPFFHIAGAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SSGQTAVRMSTGAILGSSIGYLFDGTAVPLASAMLSCGLLALAAVLFSERGRLFGRRVEG
CCCCEEEEEEHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]