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The map label for this gene is hsaB [H]

Identifier: 85374671

GI number: 85374671

Start: 1874024

End: 1874539

Strand: Direct

Name: hsaB [H]

Synonym: ELI_09220

Alternate gene names: 85374671

Gene position: 1874024-1874539 (Clockwise)

Preceding gene: 85374670

Following gene: 85374681

Centisome position: 61.4

GC content: 66.67

Gene sequence:

>516_bases
GTGAGCAAGCCGCGGACCGTGACCTTTCGTGCAGGCGACGACACCCGCGCGCTGCGCGATGCCTTCGGCTGCTTCGCGAC
CGGGGTGACGATCCTGACCACGACCGCGACCGACGGCGCGCCCGTAGGCCTGACGGTCAGCAGCTTCTCGTCGGTCAGCC
TCGACCCGCCGCTGTTGCTGGTGTGTCCCGCACTCGACGCCGGCAGCACCGCCGCGCTCGAAGGCGCAAAACGCTTTGCC
GTCAATGTCTTGGCGAGCGAGGACAAGGCGCTCTCGAGCCGTTTCGCGCGCAAGGGACTGGACCGCTTTGTCGAGAACGA
GTGGCACCGCTCCGAACACGGCCTGCCGCTGCTCGATCGCGCTCTGGCCACTTTCGAATGCGAACGCCACGCAGTGCACG
ACGGCGGCGATCACCTGATCCTTGTCGGCCGTATCCTGCGGGCTGTGTGCCACCCGCACGACGACCCGCTGCTCTATTTC
CGCAGCCGCTATCGCGGCATCCATATCGAAGATTGA

Upstream 100 bases:

>100_bases
CATGCATGGCGACGTGCTGGAGGACGTGATCATCGAAAATGTGCGATCGGTCGATTGACGCCCCCTTCCCTTGCGCCCCA
AGCCTGCTAACCCGCGGCCT

Downstream 100 bases:

>100_bases
GCCGCCAACGAAAAAGGGCGAGAACCGCAGCCCCCGCCCTTTCCTACTCGTGATCGGAAACGCTACACCACGGCGTCACG
CTTTACGGTAATCACCTGCG

Product: NADH:riboflavin 5'-phosphate oxidoreductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSKPRTVTFRAGDDTRALRDAFGCFATGVTILTTTATDGAPVGLTVSSFSSVSLDPPLLLVCPALDAGSTAALEGAKRFA
VNVLASEDKALSSRFARKGLDRFVENEWHRSEHGLPLLDRALATFECERHAVHDGGDHLILVGRILRAVCHPHDDPLLYF
RSRYRGIHIED

Sequences:

>Translated_171_residues
MSKPRTVTFRAGDDTRALRDAFGCFATGVTILTTTATDGAPVGLTVSSFSSVSLDPPLLLVCPALDAGSTAALEGAKRFA
VNVLASEDKALSSRFARKGLDRFVENEWHRSEHGLPLLDRALATFECERHAVHDGGDHLILVGRILRAVCHPHDDPLLYF
RSRYRGIHIED
>Mature_170_residues
SKPRTVTFRAGDDTRALRDAFGCFATGVTILTTTATDGAPVGLTVSSFSSVSLDPPLLLVCPALDAGSTAALEGAKRFAV
NVLASEDKALSSRFARKGLDRFVENEWHRSEHGLPLLDRALATFECERHAVHDGGDHLILVGRILRAVCHPHDDPLLYFR
SRYRGIHIED

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=32.9113924050633, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18639; Mature: 18508

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPRTVTFRAGDDTRALRDAFGCFATGVTILTTTATDGAPVGLTVSSFSSVSLDPPLLL
CCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCCCCCCCCCEEE
VCPALDAGSTAALEGAKRFAVNVLASEDKALSSRFARKGLDRFVENEWHRSEHGLPLLDR
EECCCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ALATFECERHAVHDGGDHLILVGRILRAVCHPHDDPLLYFRSRYRGIHIED
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHEEHHHCCCCEECC
>Mature Secondary Structure 
SKPRTVTFRAGDDTRALRDAFGCFATGVTILTTTATDGAPVGLTVSSFSSVSLDPPLLL
CCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCCCCCCCCCEEE
VCPALDAGSTAALEGAKRFAVNVLASEDKALSSRFARKGLDRFVENEWHRSEHGLPLLDR
EECCCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ALATFECERHAVHDGGDHLILVGRILRAVCHPHDDPLLYFRSRYRGIHIED
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHEEHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA