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The map label for this gene is ubiH [H]

Identifier: 85374655

GI number: 85374655

Start: 1859129

End: 1860349

Strand: Direct

Name: ubiH [H]

Synonym: ELI_09140

Alternate gene names: 85374655

Gene position: 1859129-1860349 (Clockwise)

Preceding gene: 85374653

Following gene: 85374659

Centisome position: 60.91

GC content: 65.6

Gene sequence:

>1221_bases
ATGGCCGAAACACGCGATCTGCTGATCCTGGGCGGCGGGCTTGTCGGGATGACGCTGGCGCTGGCAGCGGCGCGGCGCGG
CTTCACCAGCCATGTCGTCGACCGCGCCGATCCGGCGGATGTGACGGCGGACGGGTTCGACGGACGGGCCACGGCAATCT
CCACTGCAAGCTGGAACCTGTTTCGAAATATCGGCCTCGCCGACACGCTCGAACCGCATGGCTGCCCGATCGATTCCATC
GCCGTCTCCGACCAGATGAAGCCCGGCCGGATCGACTTCCAGCCGGAACCGCACGAAGACTCGCTTGGTCGGATGTTCGC
CAATCGCACCCTCAGGGTTACTCTGTTCGATGCAGCGCGCGCGGAAGAGAACATTGCGTGGCATGCTCCTGCCGAGATCG
TCGATCGCCAGCGTGACGAGTTCGGTGCCAGCGCGGCCTTGGCCGACGGCACTGTGCTGAAGGGCCACCTGATGGTCGCC
GCCGAGGGTCGCGGTTCGCCGACGCGCGAGGAGGAGGGCCTCAACCTCGCCAAATGGGATTACAGCCATCGCGCGATTAT
CGTCGGGCTCGATCACGAGAAGCCGCACGAAAACGTGGCTTGGGAGATCTTCTATCCCGACGGGCCTTTTGCGCTGCTGC
CCGTGCTCGACGGGCCAAACGGCCAGCATCGCAGCGCGCTGGTGTGGACAGTAGACGAGGCCGATGCCGAAGGCATTCTC
AAGCTGTCTGACCGCGCGTTCCTGGCCGAAGTCTACGATCGCATGGATCGCATGTTCGGCACAATCGGCCTGAATTCCAA
GCGATCATCCTATCCGCTGAGCTTCCAGCACACGTCCAGGTTGACCGGCGACCGGCTGGCTTTGGTTGGCGACGCCGCGC
ACGGCATGCATCCGATTGCGGGCCAGGGCTTCAATCTCGGCCTGCGCGATGTCGGCGCGCTGGTCGAAGTGCTGTCGGAG
GGCCGCAGGCTCGGCCTCGATCCCGGCGATGCGCAGATGCTCAAGAAATACGAGGAATGGCGCGCGCTCGATGCGCTGAT
GGTGATGTCGGCGACCGATGGCCTGACGCGGCTGTTCGGCGTGCCCGGCAAGCTCGCTTCGGCGGTGCGGCGGCTCGGCA
TGGGCGGCGTCCAGCGCACCGGCTGGCTCAAGGATTTCTTCATGAACGAAGCGCGCGGCGTGACCGGCGAGCGGCCCGAA
CTGCTGCGCCACCGCGCGTAA

Upstream 100 bases:

>100_bases
AGCGCGAACATGGGAGCGGCAATGCGGCTGAAGGTCGATCTTGCGGAGGGCATCACTTCGGTCCTACTGCGCCATGTAAG
TTTGAATGCAAAGGAATTCC

Downstream 100 bases:

>100_bases
TTTACTGCTCTACGGTTTCCTGCACCGGGCTCGGCTGGCCGATGCGGTTGCCTGCCCCATCGCGCAAGCGCCGCGGTTCG
AGCACGGCTGCGGTCTCGAT

Product: 2-polyprenyl-6-methoxyphenol hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAETRDLLILGGGLVGMTLALAAARRGFTSHVVDRADPADVTADGFDGRATAISTASWNLFRNIGLADTLEPHGCPIDSI
AVSDQMKPGRIDFQPEPHEDSLGRMFANRTLRVTLFDAARAEENIAWHAPAEIVDRQRDEFGASAALADGTVLKGHLMVA
AEGRGSPTREEEGLNLAKWDYSHRAIIVGLDHEKPHENVAWEIFYPDGPFALLPVLDGPNGQHRSALVWTVDEADAEGIL
KLSDRAFLAEVYDRMDRMFGTIGLNSKRSSYPLSFQHTSRLTGDRLALVGDAAHGMHPIAGQGFNLGLRDVGALVEVLSE
GRRLGLDPGDAQMLKKYEEWRALDALMVMSATDGLTRLFGVPGKLASAVRRLGMGGVQRTGWLKDFFMNEARGVTGERPE
LLRHRA

Sequences:

>Translated_406_residues
MAETRDLLILGGGLVGMTLALAAARRGFTSHVVDRADPADVTADGFDGRATAISTASWNLFRNIGLADTLEPHGCPIDSI
AVSDQMKPGRIDFQPEPHEDSLGRMFANRTLRVTLFDAARAEENIAWHAPAEIVDRQRDEFGASAALADGTVLKGHLMVA
AEGRGSPTREEEGLNLAKWDYSHRAIIVGLDHEKPHENVAWEIFYPDGPFALLPVLDGPNGQHRSALVWTVDEADAEGIL
KLSDRAFLAEVYDRMDRMFGTIGLNSKRSSYPLSFQHTSRLTGDRLALVGDAAHGMHPIAGQGFNLGLRDVGALVEVLSE
GRRLGLDPGDAQMLKKYEEWRALDALMVMSATDGLTRLFGVPGKLASAVRRLGMGGVQRTGWLKDFFMNEARGVTGERPE
LLRHRA
>Mature_405_residues
AETRDLLILGGGLVGMTLALAAARRGFTSHVVDRADPADVTADGFDGRATAISTASWNLFRNIGLADTLEPHGCPIDSIA
VSDQMKPGRIDFQPEPHEDSLGRMFANRTLRVTLFDAARAEENIAWHAPAEIVDRQRDEFGASAALADGTVLKGHLMVAA
EGRGSPTREEEGLNLAKWDYSHRAIIVGLDHEKPHENVAWEIFYPDGPFALLPVLDGPNGQHRSALVWTVDEADAEGILK
LSDRAFLAEVYDRMDRMFGTIGLNSKRSSYPLSFQHTSRLTGDRLALVGDAAHGMHPIAGQGFNLGLRDVGALVEVLSEG
RRLGLDPGDAQMLKKYEEWRALDALMVMSATDGLTRLFGVPGKLASAVRRLGMGGVQRTGWLKDFFMNEARGVTGERPEL
LRHRA

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=398, Percent_Identity=25.6281407035176, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI32967307, Length=311, Percent_Identity=27.6527331189711, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789274, Length=358, Percent_Identity=32.9608938547486, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1786883, Length=394, Percent_Identity=29.6954314720812, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789273, Length=404, Percent_Identity=29.2079207920792, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146631, Length=433, Percent_Identity=27.2517321016166, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321694, Length=459, Percent_Identity=24.1830065359477, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19920760, Length=390, Percent_Identity=27.6923076923077, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 44267; Mature: 44136

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETRDLLILGGGLVGMTLALAAARRGFTSHVVDRADPADVTADGFDGRATAISTASWNL
CCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHH
FRNIGLADTLEPHGCPIDSIAVSDQMKPGRIDFQPEPHEDSLGRMFANRTLRVTLFDAAR
HHCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHHHCCEEEEEEEECCC
AEENIAWHAPAEIVDRQRDEFGASAALADGTVLKGHLMVAAEGRGSPTREEEGLNLAKWD
CCCCEEECCCHHHHHHHHHHHCCCCEECCCEEEEEEEEEEECCCCCCCCHHCCCEEEEEC
YSHRAIIVGLDHEKPHENVAWEIFYPDGPFALLPVLDGPNGQHRSALVWTVDEADAEGIL
CCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCEE
KLSDRAFLAEVYDRMDRMFGTIGLNSKRSSYPLSFQHTSRLTGDRLALVGDAAHGMHPIA
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHCCCCCCEEEEEECCCCCCCCCC
GQGFNLGLRDVGALVEVLSEGRRLGLDPGDAQMLKKYEEWRALDALMVMSATDGLTRLFG
CCCCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
VPGKLASAVRRLGMGGVQRTGWLKDFFMNEARGVTGERPELLRHRA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure 
AETRDLLILGGGLVGMTLALAAARRGFTSHVVDRADPADVTADGFDGRATAISTASWNL
CCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHH
FRNIGLADTLEPHGCPIDSIAVSDQMKPGRIDFQPEPHEDSLGRMFANRTLRVTLFDAAR
HHCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHHHCCEEEEEEEECCC
AEENIAWHAPAEIVDRQRDEFGASAALADGTVLKGHLMVAAEGRGSPTREEEGLNLAKWD
CCCCEEECCCHHHHHHHHHHHCCCCEECCCEEEEEEEEEEECCCCCCCCHHCCCEEEEEC
YSHRAIIVGLDHEKPHENVAWEIFYPDGPFALLPVLDGPNGQHRSALVWTVDEADAEGIL
CCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCEE
KLSDRAFLAEVYDRMDRMFGTIGLNSKRSSYPLSFQHTSRLTGDRLALVGDAAHGMHPIA
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHCCCCCCEEEEEECCCCCCCCCC
GQGFNLGLRDVGALVEVLSEGRRLGLDPGDAQMLKKYEEWRALDALMVMSATDGLTRLFG
CCCCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
VPGKLASAVRRLGMGGVQRTGWLKDFFMNEARGVTGERPELLRHRA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]