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The map label for this gene is fixJ [H]

Identifier: 85374638

GI number: 85374638

Start: 1844466

End: 1845125

Strand: Reverse

Name: fixJ [H]

Synonym: ELI_09055

Alternate gene names: 85374638

Gene position: 1845125-1844466 (Counterclockwise)

Preceding gene: 85374639

Following gene: 85374636

Centisome position: 60.45

GC content: 63.64

Gene sequence:

>660_bases
ATGGAACACCGCCTTACCCTTCACATCATCGATAGCGACACCCGCAGCCGAGCCGAATTGGCGCGGACAGGCTTTGCCCT
TGGCCACCATGCCGAGGTCTACGGCTCGCTTCCCGAACTGTTCGAACATCATCCCCGCGAAGGTCTGATCATCGCGCGCG
ACGACCCTCGGCATGTCAGCGTAACGGAAACGATCGAGCAGATGACCGATCACGGCGTCTGGCTGCCCGTCATCGCCGCG
GATAGCCAGCCAACGACTGCGCGTATCGTAGCGGCGATAAAGGCGGGGGCGCTCGATTACGTGACCTTGCCGATCGAGGC
CAACCGCTTTGCTGCGATGCTGCGACGGACCGGCAGCGAAGCCGTGCACCAAGCCGCGATTCGGCGGCGGATGCTCGAGG
CCCGCAAGCGGATCGAGACCCTGTCCAACCGCGAGCGCGAAGTGCTCGAATGGCTGACGGAGGGCAGCAGCAACAAGGCC
ATTGCGCGCGAACTCGATATCAGTCCGCGGACGGTCGAAATCCATCGCGCCAACATGATGACCAAGCTCGGGGCCCGCCA
TGCTGCCGAGGCTGTGCGACTCAGGCTGGAAGCCCAGATCGAGCATTCCTTGGAGAGTGGGGGGGCGGAGTCCGAACTCC
GTCGCAGCTTGGCAAGCTAG

Upstream 100 bases:

>100_bases
GATGAGTCTTCGCAATTCCCGAATTCGATTTGTCCGATTGCATCCAGTATCGGCGTATTGCGAATTTTTGAGGCGCCGGT
TGCGCTCCATCGTTCCAGGC

Downstream 100 bases:

>100_bases
CGATCATGCGTGCCGCTCTTCGCAGCGGCCCCTAACGGCAGAATTTCGAGCTACCCCATTCGAGGTGCAGATGGTCGCGG
TGCGCCGAATTGTAATCGGG

Product: LuxR family transcriptional regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MEHRLTLHIIDSDTRSRAELARTGFALGHHAEVYGSLPELFEHHPREGLIIARDDPRHVSVTETIEQMTDHGVWLPVIAA
DSQPTTARIVAAIKAGALDYVTLPIEANRFAAMLRRTGSEAVHQAAIRRRMLEARKRIETLSNREREVLEWLTEGSSNKA
IARELDISPRTVEIHRANMMTKLGARHAAEAVRLRLEAQIEHSLESGGAESELRRSLAS

Sequences:

>Translated_219_residues
MEHRLTLHIIDSDTRSRAELARTGFALGHHAEVYGSLPELFEHHPREGLIIARDDPRHVSVTETIEQMTDHGVWLPVIAA
DSQPTTARIVAAIKAGALDYVTLPIEANRFAAMLRRTGSEAVHQAAIRRRMLEARKRIETLSNREREVLEWLTEGSSNKA
IARELDISPRTVEIHRANMMTKLGARHAAEAVRLRLEAQIEHSLESGGAESELRRSLAS
>Mature_219_residues
MEHRLTLHIIDSDTRSRAELARTGFALGHHAEVYGSLPELFEHHPREGLIIARDDPRHVSVTETIEQMTDHGVWLPVIAA
DSQPTTARIVAAIKAGALDYVTLPIEANRFAAMLRRTGSEAVHQAAIRRRMLEARKRIETLSNREREVLEWLTEGSSNKA
IARELDISPRTVEIHRANMMTKLGARHAAEAVRLRLEAQIEHSLESGGAESELRRSLAS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24521; Mature: 24521

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHRLTLHIIDSDTRSRAELARTGFALGHHAEVYGSLPELFEHHPREGLIIARDDPRHVS
CCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCCEEEECCCCCCCH
VTETIEQMTDHGVWLPVIAADSQPTTARIVAAIKAGALDYVTLPIEANRFAAMLRRTGSE
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCHH
AVHQAAIRRRMLEARKRIETLSNREREVLEWLTEGSSNKAIARELDISPRTVEIHRANMM
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEHHHHHH
TKLGARHAAEAVRLRLEAQIEHSLESGGAESELRRSLAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MEHRLTLHIIDSDTRSRAELARTGFALGHHAEVYGSLPELFEHHPREGLIIARDDPRHVS
CCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCCEEEECCCCCCCH
VTETIEQMTDHGVWLPVIAADSQPTTARIVAAIKAGALDYVTLPIEANRFAAMLRRTGSE
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCHH
AVHQAAIRRRMLEARKRIETLSNREREVLEWLTEGSSNKAIARELDISPRTVEIHRANMM
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEHHHHHH
TKLGARHAAEAVRLRLEAQIEHSLESGGAESELRRSLAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]