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The map label for this gene is opuE [H]

Identifier: 85374634

GI number: 85374634

Start: 1840932

End: 1842434

Strand: Direct

Name: opuE [H]

Synonym: ELI_09035

Alternate gene names: 85374634

Gene position: 1840932-1842434 (Clockwise)

Preceding gene: 85374631

Following gene: 85374635

Centisome position: 60.31

GC content: 66.67

Gene sequence:

>1503_bases
ATGACTGAAGGCACCATGCAAACCGGCACACTCGTCTCGCTCGCGCTCTATTTCATCCTGATGCTCGCCATCGGGCTCTA
TGCCTGGCGCAAATCGACCTCCGACAGCGCGGGCTATCTGCTCGCGGGGCGCAATTTGCCGCCGTCGGTGGCGGCGCTTT
CTGCCGGGGCGTCGGACATGTCGGGCTGGTTGCTGTTAGGGCTGCCGGGGGCGCTTTATGCCAGCGGGCTGGTCGAGGCG
TGGATCGGGATCGGGCTGTTCGTCGGCGCGGTCGCCAACTGGATCGTGGTCGCCCCGCGCCTGCGCCAGCAGACCGAGGA
GCTGGGCAATGCGCTCACCATCCCAGAATTCCTCGCCAACCGCTTTCCCAGCCAGGCCGTCGCGCTGCGCGTGACGAGCG
CGGTGATCATCGTGCTGTTCTTCACCGTCTATACCGCCGCGGGCCTGGTCGGCGGGGGCAAGTTGTTCGAGACCGCCTTT
GCCGGCATGCTGCCCGGTATGGGCATGAGCGATTATATGCTCGGCATCTGGATCACGGCGGGCGTGGTGCTGGCCTACAC
GATGATCGGCGGTTTCCTGGCCGTAAGCCTGACCGACTTCGTGCAGGGCTGCATCATGGTGGTCGCGCTGGTGCTGATGC
CGCTGGTGGTGCTGACCGGCGGGGTGGATCTTGGCTCCGCCTCCGAAGCGGCGGCAGCGGCGGGCAACAGCGATTACCTC
GCGCTGTTCGGCGGGCTGACCGCGATCGGCTGGCTCAGCGCCGTGACCTGGGGCCTCGGCTATTTCGGGCAACCGCATAT
CATCGTGCGATTCATGGCCATCCGCAGCATCCCCGAGGTCAAGACCGCGCGCAACATCGGGCTCACATGGATGGGTGTCG
CGCTGATCGGGGCGATCGGCGTCGGGATAGCCGGGCGCGCCTATGCCGAGGCAAATGGCGTAGCAGTGGAGGATCCCGAA
ACGATCTTCATCGTCCTTGCCACGCTGCTGTTCCACCCGCTGATCACCGGCTTCCTGCTGGCCGCATTGCTGGCGGCGAT
CATGAGCACGATCAGTTCGCAATTGCTGGTCAGTTCCAGCAGCCTGACCGAGGACTTCTATCGCCTGTTCCTGCGCAAAC
ACGCGAGCGAGCGAGAGGCCGTCAATGTCGGGCGTGTCTGCGTGTTGCTGGTGGCGCTGGCGGCAATCGTGATCGCGCGC
GATCCGGAGAGCCAGGTGCTCGGCCTCGTCGCCAACGCCTGGGCCGGGTTTGGCGCTGCGTTCGGCCCGCTGATCCTGTT
TGCGCTGACATGGGACCGGATGACCGGCGCGGGCGCGGTGGCGGGCCTGGTGACGGGCGCGGTCGTCGTGGTCGCATGGA
TCGCGCTGGGCTGGAACGGCGCGTTTCTGGGCGGACCGGGGGTCTACGAGATCATCCCCGGCTTCATCGCGGCGAGCCTG
GCCATCTGGCTTGTTTCGAAGGCGACGGCGCGCGGCGAGGAGCCGGTGGCGCAGGCGGGCTGA

Upstream 100 bases:

>100_bases
TTCGGCACCAACGCGTAATCGCTCGGTGGTCTGACCCGCAGCGGAGTTCCCCCATCGCACTGCTGGTCACGGTACCGCCC
ACCTGTTATCCGCGCTCTCC

Downstream 100 bases:

>100_bases
TCCCGCCCGTTTCGCACTCACCGCCCGCTGGGCTAGGGTCGGGTCTCGAGAGCTCGAGGAGGAGACGATCATGGCAAAAG
TGACCGGACTGGGCGGCGTT

Product: proline/sodium symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTEGTMQTGTLVSLALYFILMLAIGLYAWRKSTSDSAGYLLAGRNLPPSVAALSAGASDMSGWLLLGLPGALYASGLVEA
WIGIGLFVGAVANWIVVAPRLRQQTEELGNALTIPEFLANRFPSQAVALRVTSAVIIVLFFTVYTAAGLVGGGKLFETAF
AGMLPGMGMSDYMLGIWITAGVVLAYTMIGGFLAVSLTDFVQGCIMVVALVLMPLVVLTGGVDLGSASEAAAAAGNSDYL
ALFGGLTAIGWLSAVTWGLGYFGQPHIIVRFMAIRSIPEVKTARNIGLTWMGVALIGAIGVGIAGRAYAEANGVAVEDPE
TIFIVLATLLFHPLITGFLLAALLAAIMSTISSQLLVSSSSLTEDFYRLFLRKHASEREAVNVGRVCVLLVALAAIVIAR
DPESQVLGLVANAWAGFGAAFGPLILFALTWDRMTGAGAVAGLVTGAVVVVAWIALGWNGAFLGGPGVYEIIPGFIAASL
AIWLVSKATARGEEPVAQAG

Sequences:

>Translated_500_residues
MTEGTMQTGTLVSLALYFILMLAIGLYAWRKSTSDSAGYLLAGRNLPPSVAALSAGASDMSGWLLLGLPGALYASGLVEA
WIGIGLFVGAVANWIVVAPRLRQQTEELGNALTIPEFLANRFPSQAVALRVTSAVIIVLFFTVYTAAGLVGGGKLFETAF
AGMLPGMGMSDYMLGIWITAGVVLAYTMIGGFLAVSLTDFVQGCIMVVALVLMPLVVLTGGVDLGSASEAAAAAGNSDYL
ALFGGLTAIGWLSAVTWGLGYFGQPHIIVRFMAIRSIPEVKTARNIGLTWMGVALIGAIGVGIAGRAYAEANGVAVEDPE
TIFIVLATLLFHPLITGFLLAALLAAIMSTISSQLLVSSSSLTEDFYRLFLRKHASEREAVNVGRVCVLLVALAAIVIAR
DPESQVLGLVANAWAGFGAAFGPLILFALTWDRMTGAGAVAGLVTGAVVVVAWIALGWNGAFLGGPGVYEIIPGFIAASL
AIWLVSKATARGEEPVAQAG
>Mature_499_residues
TEGTMQTGTLVSLALYFILMLAIGLYAWRKSTSDSAGYLLAGRNLPPSVAALSAGASDMSGWLLLGLPGALYASGLVEAW
IGIGLFVGAVANWIVVAPRLRQQTEELGNALTIPEFLANRFPSQAVALRVTSAVIIVLFFTVYTAAGLVGGGKLFETAFA
GMLPGMGMSDYMLGIWITAGVVLAYTMIGGFLAVSLTDFVQGCIMVVALVLMPLVVLTGGVDLGSASEAAAAAGNSDYLA
LFGGLTAIGWLSAVTWGLGYFGQPHIIVRFMAIRSIPEVKTARNIGLTWMGVALIGAIGVGIAGRAYAEANGVAVEDPET
IFIVLATLLFHPLITGFLLAALLAAIMSTISSQLLVSSSSLTEDFYRLFLRKHASEREAVNVGRVCVLLVALAAIVIARD
PESQVLGLVANAWAGFGAAFGPLILFALTWDRMTGAGAVAGLVTGAVVVVAWIALGWNGAFLGGPGVYEIIPGFIAASLA
IWLVSKATARGEEPVAQAG

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=499, Percent_Identity=47.4949899799599, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI110835708, Length=529, Percent_Identity=24.007561436673, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1787251, Length=483, Percent_Identity=50.3105590062112, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790503, Length=506, Percent_Identity=25.098814229249, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082237, Length=468, Percent_Identity=26.7094017094017, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539284, Length=424, Percent_Identity=25.7075471698113, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 51794; Mature: 51662

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGTMQTGTLVSLALYFILMLAIGLYAWRKSTSDSAGYLLAGRNLPPSVAALSAGASDM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCCC
SGWLLLGLPGALYASGLVEAWIGIGLFVGAVANWIVVAPRLRQQTEELGNALTIPEFLAN
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
RFPSQAVALRVTSAVIIVLFFTVYTAAGLVGGGKLFETAFAGMLPGMGMSDYMLGIWITA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GVVLAYTMIGGFLAVSLTDFVQGCIMVVALVLMPLVVLTGGVDLGSASEAAAAAGNSDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHH
ALFGGLTAIGWLSAVTWGLGYFGQPHIIVRFMAIRSIPEVKTARNIGLTWMGVALIGAIG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
VGIAGRAYAEANGVAVEDPETIFIVLATLLFHPLITGFLLAALLAAIMSTISSQLLVSSS
HCCCCCHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SLTEDFYRLFLRKHASEREAVNVGRVCVLLVALAAIVIARDPESQVLGLVANAWAGFGAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FGPLILFALTWDRMTGAGAVAGLVTGAVVVVAWIALGWNGAFLGGPGVYEIIPGFIAASL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIWLVSKATARGEEPVAQAG
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TEGTMQTGTLVSLALYFILMLAIGLYAWRKSTSDSAGYLLAGRNLPPSVAALSAGASDM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCCC
SGWLLLGLPGALYASGLVEAWIGIGLFVGAVANWIVVAPRLRQQTEELGNALTIPEFLAN
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
RFPSQAVALRVTSAVIIVLFFTVYTAAGLVGGGKLFETAFAGMLPGMGMSDYMLGIWITA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GVVLAYTMIGGFLAVSLTDFVQGCIMVVALVLMPLVVLTGGVDLGSASEAAAAAGNSDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHH
ALFGGLTAIGWLSAVTWGLGYFGQPHIIVRFMAIRSIPEVKTARNIGLTWMGVALIGAIG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
VGIAGRAYAEANGVAVEDPETIFIVLATLLFHPLITGFLLAALLAAIMSTISSQLLVSSS
HCCCCCHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SLTEDFYRLFLRKHASEREAVNVGRVCVLLVALAAIVIARDPESQVLGLVANAWAGFGAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FGPLILFALTWDRMTGAGAVAGLVTGAVVVVAWIALGWNGAFLGGPGVYEIIPGFIAASL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIWLVSKATARGEEPVAQAG
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]