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The map label for this gene is recF

Identifier: 85374625

GI number: 85374625

Start: 1826844

End: 1827929

Strand: Direct

Name: recF

Synonym: ELI_08990

Alternate gene names: 85374625

Gene position: 1826844-1827929 (Clockwise)

Preceding gene: 85374620

Following gene: 85374627

Centisome position: 59.85

GC content: 67.96

Gene sequence:

>1086_bases
ATGGCGCTCGATCGCATCACCCTTTCCAACCTTCGCAACCACGCTGAAACCCGGCTACAAGGAACCGCGCGCTTCAATCT
GTTGGTCGGCGAGAACGGCGCCGGGAAGACCAATGTGCTGGAGGCGATTTCATTGCTCGCGCCCGGGCGCGGTCTGCGCC
GGGCGGCCCTGCCTGACATAGCGCGTGCGGGCGGTGGCGGTGGCTTCACCGTGGGTGCCAGCCTGACACCGGGCGATGGC
GGGGAGCCGGTTCAATTGGGCACCATGGTCGATCCGGCCCGCCCCGGACGGCGGCGGGTGCGCGTCAATGGCGCGGAAGC
GAGCGCTGTCTCGCTCGGCGAATGGCTCGCGATCGGCTGGTTGACCCCGGCGATGGACGGCATCTTCATGGGCCCCGCCG
GCGACCGGCGGCGTTTTGTGGACCGCATGGCGCTGGCCCTCGACCCGCTCCACGCGCGCCACGCCAGTCGCTATGAGAAT
GTCCTGCGCGAGCGGAACCGCCTGCTTGGCGACGAGCGCGAGCCCGACCCCACCTGGCTCGACGGGATCGAAGCGCAAAT
GGCCAAGCACGGGAGCGCGCTGGCGCAAAGCCGCGCGCGACTGGTAGGCGTTTTGGTGGAGACGCTCGCCTCGCACCCCG
ACGAGCCATTTGCCCGCCCGTTACTCGCGATCCTGACCGAGAGCCCTTTCGAGGAAGAAGCTTTACGCGTCGCCCTGCGC
GCTAATCGCGGGCGGGATCGCCGCGCCGGTCGCACCCTCATCGGGCCGCACCGTGACGATCTGACAGTCACCATGGCCGG
GAAAGATACGCCCGCCGCTTCGTGCTCCACAGGCGAGCAGAAAGCGATGTTGATTGCCATCACGCTGGCTCATGGCGAAC
TGGCCGCGCGCGGCAGGCCTGGCGTGATGCTGCTCGATGAAGTGGCCGCCCATATCGACCCCGTCCGGCGGGAGGCGCTG
TTCGAGCGCCTCCGCGAGACTGGCGCTCAGGTCTGGATGACAGGGACCGAATTGGCGCCGTTTGCTGCGATCGAAGCCGA
AGCGGCGGTGTGGCGGGTGAGTGGCGGAAGTCTGGAGAAATTGTGA

Upstream 100 bases:

>100_bases
TCGTTCATGATTCCGAAAGGTTGGCATATTTTCCCAATTGGTGGGATTGGGCTTTTTCGTAGCCTTGAGAGCGGGCGCGC
GCTAACCGCCCGACAGTCCC

Downstream 100 bases:

>100_bases
GACCCCGCGTCGGCGGGGACCTCAGGCAGGTGGAAACCTACAAGCCAACGACCAGGAGGACTGAGACCCCCGCCTGCGCG
GGGGATCGCGTCAGTTTGTC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MALDRITLSNLRNHAETRLQGTARFNLLVGENGAGKTNVLEAISLLAPGRGLRRAALPDIARAGGGGGFTVGASLTPGDG
GEPVQLGTMVDPARPGRRRVRVNGAEASAVSLGEWLAIGWLTPAMDGIFMGPAGDRRRFVDRMALALDPLHARHASRYEN
VLRERNRLLGDEREPDPTWLDGIEAQMAKHGSALAQSRARLVGVLVETLASHPDEPFARPLLAILTESPFEEEALRVALR
ANRGRDRRAGRTLIGPHRDDLTVTMAGKDTPAASCSTGEQKAMLIAITLAHGELAARGRPGVMLLDEVAAHIDPVRREAL
FERLRETGAQVWMTGTELAPFAAIEAEAAVWRVSGGSLEKL

Sequences:

>Translated_361_residues
MALDRITLSNLRNHAETRLQGTARFNLLVGENGAGKTNVLEAISLLAPGRGLRRAALPDIARAGGGGGFTVGASLTPGDG
GEPVQLGTMVDPARPGRRRVRVNGAEASAVSLGEWLAIGWLTPAMDGIFMGPAGDRRRFVDRMALALDPLHARHASRYEN
VLRERNRLLGDEREPDPTWLDGIEAQMAKHGSALAQSRARLVGVLVETLASHPDEPFARPLLAILTESPFEEEALRVALR
ANRGRDRRAGRTLIGPHRDDLTVTMAGKDTPAASCSTGEQKAMLIAITLAHGELAARGRPGVMLLDEVAAHIDPVRREAL
FERLRETGAQVWMTGTELAPFAAIEAEAAVWRVSGGSLEKL
>Mature_360_residues
ALDRITLSNLRNHAETRLQGTARFNLLVGENGAGKTNVLEAISLLAPGRGLRRAALPDIARAGGGGGFTVGASLTPGDGG
EPVQLGTMVDPARPGRRRVRVNGAEASAVSLGEWLAIGWLTPAMDGIFMGPAGDRRRFVDRMALALDPLHARHASRYENV
LRERNRLLGDEREPDPTWLDGIEAQMAKHGSALAQSRARLVGVLVETLASHPDEPFARPLLAILTESPFEEEALRVALRA
NRGRDRRAGRTLIGPHRDDLTVTMAGKDTPAASCSTGEQKAMLIAITLAHGELAARGRPGVMLLDEVAAHIDPVRREALF
ERLRETGAQVWMTGTELAPFAAIEAEAAVWRVSGGSLEKL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=338, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 38724; Mature: 38593

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDRITLSNLRNHAETRLQGTARFNLLVGENGAGKTNVLEAISLLAPGRGLRRAALPDI
CCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCHH
ARAGGGGGFTVGASLTPGDGGEPVQLGTMVDPARPGRRRVRVNGAEASAVSLGEWLAIGW
HHCCCCCCEEECCEECCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LTPAMDGIFMGPAGDRRRFVDRMALALDPLHARHASRYENVLRERNRLLGDEREPDPTWL
HHHHHCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
DGIEAQMAKHGSALAQSRARLVGVLVETLASHPDEPFARPLLAILTESPFEEEALRVALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
ANRGRDRRAGRTLIGPHRDDLTVTMAGKDTPAASCSTGEQKAMLIAITLAHGELAARGRP
HCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCHHCCCCC
GVMLLDEVAAHIDPVRREALFERLRETGAQVWMTGTELAPFAAIEAEAAVWRVSGGSLEK
CEEHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHEECCCEEEEECCCCCCC
L
C
>Mature Secondary Structure 
ALDRITLSNLRNHAETRLQGTARFNLLVGENGAGKTNVLEAISLLAPGRGLRRAALPDI
CCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCHH
ARAGGGGGFTVGASLTPGDGGEPVQLGTMVDPARPGRRRVRVNGAEASAVSLGEWLAIGW
HHCCCCCCEEECCEECCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LTPAMDGIFMGPAGDRRRFVDRMALALDPLHARHASRYENVLRERNRLLGDEREPDPTWL
HHHHHCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
DGIEAQMAKHGSALAQSRARLVGVLVETLASHPDEPFARPLLAILTESPFEEEALRVALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
ANRGRDRRAGRTLIGPHRDDLTVTMAGKDTPAASCSTGEQKAMLIAITLAHGELAARGRP
HCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCHHCCCCC
GVMLLDEVAAHIDPVRREALFERLRETGAQVWMTGTELAPFAAIEAEAAVWRVSGGSLEK
CEEHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHEECCCEEEEECCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA