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The map label for this gene is rlmN

Identifier: 85374384

GI number: 85374384

Start: 1612519

End: 1613775

Strand: Direct

Name: rlmN

Synonym: ELI_07785

Alternate gene names: 85374384

Gene position: 1612519-1613775 (Clockwise)

Preceding gene: 85374382

Following gene: 85374385

Centisome position: 52.83

GC content: 63.25

Gene sequence:

>1257_bases
ATGGCCGATACGTCTCTCATGCCGATCCCCGGACAGGTGGATCCCGTTCCCGCGCCGCGTGACATCACACCGCGCGCCGA
TGGTCGTGTCGACCTGATGGGCCTGCCCAAGGCGCGCATACAGGAATTGTTCGCGGAGGCCGGACTCGACGCCAAGCAGG
CCAAGCTTCGCAGCAAGCAGGTCTATCACTGGCTCTATCATCGCGGCGTGACCGATTTCGAAGCGATGACCGATATCGCC
AAGACCATGCGCCCATGGCTGGCCGAGCGCTTTATCGTCGGGCGGCCCAATGTGGTCGAGGCGCAGCATTCGACCGACGG
CACACGCAAGTGGCTGCTGCAGACCGACGACGGCCACGATTTCGAGATGGTGTTCATCCCCGATGCCGATCGCGGAACAC
TGTGCGTTTCGAGCCAGGTCGGCTGCACGCTCAATTGTCGCTTCTGCCACACCGGCACGATGCGGCTGGTGCGCAACCTG
ACGCCGGGCGAGATCGTCGGCCAGGTCATGCTCGCGCGCGATGCGCTCGGCGAATGGCCCAAAGGCCGGATGGACGGGCT
CGACGATGTCGAGGACACCGGTCACTATTCAGCCGACGGCCGCCTGCTCACCAATATCGTGATGATGGGCATGGGCGAGC
CGCTGTACAATTTCGACAATGTTCGCGACGCGCTCAAGCTGGTCATGGACGGTGAAGGGCTCGCTTTGTCGAAGCGCCGC
ATTACGCTTTCGACCAGCGGCGTCGTTCCCATGATGGAGCGCTGCGGCGAGGAGATCGGCGTAAACCTCGCCGTCTCGCT
GCACGCCGTCACCAAGGACATCCGCGACGAGATCGTACCGATCAACAAGAAATACGGCATCGAGGAATTGCTGCAGGCCT
GCGCCGACTATCCCGGCGCGTCGAATGCCCGCCGCATCACCTTCGAATATGTCATGCTCAAGGACAAAAACGACACCGAC
GAGCATGCGCGCGAACTGGTGCGGCTGCTCAAGCAGTACAATCTGCCGGCAAAGGTGAACCTGATCCCCTTCAACCCCTG
GCCCGGCGCGGCCTATGAATGCTCGACGCCGGAGCGAATCAGGGCTTTCAGCAATATCGTCTTCGAAGGCGGCATCAGCG
CCCCGGTCCGCACGCCGCGCGGGCGCGATATCGACGCGGCCTGCGGGCAGCTCAAGACCGCCGCCCAGAAAAAGAGCCGC
GCCGAGCGTGATCGAGAAGCGGCAGCCGAAGCGGAGGCCGCTGCATCCCAAGCTTGA

Upstream 100 bases:

>100_bases
ACCGTCGGCCACTTCGAGACTTGGCGCGCGGGGCGCGCGTCCCCCGCGCCACCGGATCATCTGGATTTACCGGCTGGCCG
CGCCTATATGCGCGCCTCCC

Downstream 100 bases:

>100_bases
TCGGTTGCGTATCTCCCTTATCCAAACAATGACGTGGGGAGACGTTGCCATGAAAATTCGACACCTGACCGCTGCCATCG
CGCTCGCAATAGTTGCGCCC

Product: Fe-S-cluster redox protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MADTSLMPIPGQVDPVPAPRDITPRADGRVDLMGLPKARIQELFAEAGLDAKQAKLRSKQVYHWLYHRGVTDFEAMTDIA
KTMRPWLAERFIVGRPNVVEAQHSTDGTRKWLLQTDDGHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNL
TPGEIVGQVMLARDALGEWPKGRMDGLDDVEDTGHYSADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDGEGLALSKRR
ITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDIRDEIVPINKKYGIEELLQACADYPGASNARRITFEYVMLKDKNDTD
EHARELVRLLKQYNLPAKVNLIPFNPWPGAAYECSTPERIRAFSNIVFEGGISAPVRTPRGRDIDAACGQLKTAAQKKSR
AERDREAAAEAEAAASQA

Sequences:

>Translated_418_residues
MADTSLMPIPGQVDPVPAPRDITPRADGRVDLMGLPKARIQELFAEAGLDAKQAKLRSKQVYHWLYHRGVTDFEAMTDIA
KTMRPWLAERFIVGRPNVVEAQHSTDGTRKWLLQTDDGHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNL
TPGEIVGQVMLARDALGEWPKGRMDGLDDVEDTGHYSADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDGEGLALSKRR
ITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDIRDEIVPINKKYGIEELLQACADYPGASNARRITFEYVMLKDKNDTD
EHARELVRLLKQYNLPAKVNLIPFNPWPGAAYECSTPERIRAFSNIVFEGGISAPVRTPRGRDIDAACGQLKTAAQKKSR
AERDREAAAEAEAAASQA
>Mature_417_residues
ADTSLMPIPGQVDPVPAPRDITPRADGRVDLMGLPKARIQELFAEAGLDAKQAKLRSKQVYHWLYHRGVTDFEAMTDIAK
TMRPWLAERFIVGRPNVVEAQHSTDGTRKWLLQTDDGHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLT
PGEIVGQVMLARDALGEWPKGRMDGLDDVEDTGHYSADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDGEGLALSKRRI
TLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDIRDEIVPINKKYGIEELLQACADYPGASNARRITFEYVMLKDKNDTDE
HARELVRLLKQYNLPAKVNLIPFNPWPGAAYECSTPERIRAFSNIVFEGGISAPVRTPRGRDIDAACGQLKTAAQKKSRA
ERDREAAAEAEAAASQA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=376, Percent_Identity=47.3404255319149, Blast_Score=321, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ERYLH (Q2N9J2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458446.1
- STRING:   Q2N9J2
- GeneID:   3871222
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07785
- NMPDR:   fig|314225.3.peg.1485
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2N9J2
- ProtClustDB:   CLSK835312
- BioCyc:   ELIT314225:ELI_07785-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 46282; Mature: 46151

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSLMPIPGQVDPVPAPRDITPRADGRVDLMGLPKARIQELFAEAGLDAKQAKLRSKQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHH
VYHWLYHRGVTDFEAMTDIAKTMRPWLAERFIVGRPNVVEAQHSTDGTRKWLLQTDDGHD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCEEEEEECCCCCC
FEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARDALGEWP
EEEEEECCCCCCCEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KGRMDGLDDVEDTGHYSADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDGEGLALSKRR
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECEE
ITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDIRDEIVPINKKYGIEELLQACADYPGA
EEEECCCCHHHHHHCCHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
SNARRITFEYVMLKDKNDTDEHARELVRLLKQYNLPAKVNLIPFNPWPGAAYECSTPERI
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCHHHH
RAFSNIVFEGGISAPVRTPRGRDIDAACGQLKTAAQKKSRAERDREAAAEAEAAASQA
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADTSLMPIPGQVDPVPAPRDITPRADGRVDLMGLPKARIQELFAEAGLDAKQAKLRSKQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHH
VYHWLYHRGVTDFEAMTDIAKTMRPWLAERFIVGRPNVVEAQHSTDGTRKWLLQTDDGHD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCEEEEEECCCCCC
FEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARDALGEWP
EEEEEECCCCCCCEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KGRMDGLDDVEDTGHYSADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDGEGLALSKRR
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECEE
ITLSTSGVVPMMERCGEEIGVNLAVSLHAVTKDIRDEIVPINKKYGIEELLQACADYPGA
EEEECCCCHHHHHHCCHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
SNARRITFEYVMLKDKNDTDEHARELVRLLKQYNLPAKVNLIPFNPWPGAAYECSTPERI
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCHHHH
RAFSNIVFEGGISAPVRTPRGRDIDAACGQLKTAAQKKSRAERDREAAAEAEAAASQA
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA