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The map label for this gene is kefB [H]

Identifier: 85374369

GI number: 85374369

Start: 1595283

End: 1597043

Strand: Direct

Name: kefB [H]

Synonym: ELI_07710

Alternate gene names: 85374369

Gene position: 1595283-1597043 (Clockwise)

Preceding gene: 85374368

Following gene: 85374370

Centisome position: 52.26

GC content: 62.58

Gene sequence:

>1761_bases
ATGGCCGGTGAACTCTCCCCCTCCCCGATTTTGCAGGACGCGCTCGTAATCCTTGGTGCGGCGGGGATCGTCATTCCGGT
CTTCACCCGTTTCAAGATAACGCCGGTCATCGGTTTCATCCTGATCGGCGTGCTGGTCGGGCCATTCGGCCTTGGCGCAT
GGGTCTACGAATACGACTGGCTCGAATACATAACGATATCCGATCCCGAAGGGCTCGATCCCTTCGCGGAGTTCGGCATC
ATATTGCTGTTGTTCGCGATCGGACTGGAATTGTCCTTCAAGCGATTGTGGACGTTGCGAAAGCTGGTGTTCGGACTTGG
CGCGATGGAACTGCTGGTGATCGGCACCTGTGCCGCTGCGGTCTTGGCCATGATGGGGCAGTATTGGGTCGGTGCGCTGG
CCTTGGGATTCGCTCTCGCCTTCTCCTCGACCGCGATCGTCCTCCCGATCTCCGGCACGAAGACTCCGGTCGGCCGCGCG
GCGCTCTCCATGCTGCTGTTCGAAGATATCATGATCGTCCCGATCGTGTTTATCCTTGGTGCTCTCGCGCCCTTCGCGCA
GAGCGACGGTGTCACCGGCCTTGTCGATACATTATGGCAGGGTGGATTGGTGATCGTGGCCTTGCTGGTCCTCGGCCGGA
TCGCGCTGCCCCGTCTGTTCGCCCAGGCCGCGCGCACGAAGAGCCCCGAACTGTTTCTCGCCGCGTCCCTGCTGGTCGTA
ATCGGATCAAGCCTGGCAACCGGGGTTACCGGCCTTTCGCCGATTGTCGGCGCGTTGATCGCCGGCCTGCTGATCGCGGA
AACCGAGTATCACGGCGAGGTCGAATCGATCATGGAGCCGTTCAAGGGACTGGCTCTGGGAATTTTCCTCATCACCGTTG
GCATGAGTATCGATCTCGCCAGCATCTGGGACAACCTCTGGCCGATTCTCACCGCCGTCGTCCTGGTGCTCGTGTTCAAG
GCGATTGTCACGGGCACGCTCCTGCGCCTGATGGGCGCACGTCGCAGCACTGCGGCCGAAACCGGCGTGCTGATGGCCAG
CCCCTCGGAAACGACACTGATCGTGCTTTCGGCGGCGGGTTCGGCCATGCTGATCCAGCCCGGCACACAGCAATTCTGGC
AGATCGTGACCGCCATCGGCCTCACGATCACACCTTTGCTCGCCATGCTGGGGCGCGTCATTGCACGGCGGGTCGAGCCG
AACCCGTCGCTTGATGTCGGCGAAAGCGACGAGCCGCGCGTGATCATCATCGGCGTCGGGCGCGTCGGCATGCTTGTAGC
GGACATGCTCGAAGCACACGAGCGCCCCTATGTCGCCATCGATAGCAATGCCGACCTGATCGCCCGAGCCCGCCGCCGCG
GCTATCGCGCCGTTTACGGAGATGCCGCGCGGGGCGATGCGCTCGACAAGCTTGGCGTGGAGAACTCACCCGCCGTGGTG
TTGACCATGGACGAGCCCGTTCTAACCGACCGGCTGACCAGCAAATTGCGCAAGGCATATCCCGATCTGTTGATCGTCAC
CCGCGCTCGTGACAGCCAGCATGCGGCCGATCTTTACCGAGCAGGCGCCAGCCACGCCGTGCCGGAGACGCTCGAAAGCT
CGCTGCAGCTGTCCGAGGCGGTGCTCGCAGACATCGGTGTGCCCATGGGGCCGGTCATCGCATCGATCCATGCCAAGCGC
GATGAGTTCCGCGACAAGATCCAGCGGGACGCAGGCCTTGCCGAACGACCGAAATTGCGCACGAGTTCGATCCAGGGGTG
A

Upstream 100 bases:

>100_bases
GGCGAGGACAGCGAAGCACCATCGGCAGGGCCGCACGGCGGTCGTGGAGGATAGGCCATTCGCGACGTGACCTGTTCGGC
AGGCGCGCTATAGGGGGTGC

Downstream 100 bases:

>100_bases
TTTCTCCGAACCTTTTCATGCGGCTGTGCGTTGGAAGGGAAAGGAGGATTTTATGGCCGACAAAACCGACCCCACCAAAC
CGATTTCGAATGACGAGAAG

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAGELSPSPILQDALVILGAAGIVIPVFTRFKITPVIGFILIGVLVGPFGLGAWVYEYDWLEYITISDPEGLDPFAEFGI
ILLLFAIGLELSFKRLWTLRKLVFGLGAMELLVIGTCAAAVLAMMGQYWVGALALGFALAFSSTAIVLPISGTKTPVGRA
ALSMLLFEDIMIVPIVFILGALAPFAQSDGVTGLVDTLWQGGLVIVALLVLGRIALPRLFAQAARTKSPELFLAASLLVV
IGSSLATGVTGLSPIVGALIAGLLIAETEYHGEVESIMEPFKGLALGIFLITVGMSIDLASIWDNLWPILTAVVLVLVFK
AIVTGTLLRLMGARRSTAAETGVLMASPSETTLIVLSAAGSAMLIQPGTQQFWQIVTAIGLTITPLLAMLGRVIARRVEP
NPSLDVGESDEPRVIIIGVGRVGMLVADMLEAHERPYVAIDSNADLIARARRRGYRAVYGDAARGDALDKLGVENSPAVV
LTMDEPVLTDRLTSKLRKAYPDLLIVTRARDSQHAADLYRAGASHAVPETLESSLQLSEAVLADIGVPMGPVIASIHAKR
DEFRDKIQRDAGLAERPKLRTSSIQG

Sequences:

>Translated_586_residues
MAGELSPSPILQDALVILGAAGIVIPVFTRFKITPVIGFILIGVLVGPFGLGAWVYEYDWLEYITISDPEGLDPFAEFGI
ILLLFAIGLELSFKRLWTLRKLVFGLGAMELLVIGTCAAAVLAMMGQYWVGALALGFALAFSSTAIVLPISGTKTPVGRA
ALSMLLFEDIMIVPIVFILGALAPFAQSDGVTGLVDTLWQGGLVIVALLVLGRIALPRLFAQAARTKSPELFLAASLLVV
IGSSLATGVTGLSPIVGALIAGLLIAETEYHGEVESIMEPFKGLALGIFLITVGMSIDLASIWDNLWPILTAVVLVLVFK
AIVTGTLLRLMGARRSTAAETGVLMASPSETTLIVLSAAGSAMLIQPGTQQFWQIVTAIGLTITPLLAMLGRVIARRVEP
NPSLDVGESDEPRVIIIGVGRVGMLVADMLEAHERPYVAIDSNADLIARARRRGYRAVYGDAARGDALDKLGVENSPAVV
LTMDEPVLTDRLTSKLRKAYPDLLIVTRARDSQHAADLYRAGASHAVPETLESSLQLSEAVLADIGVPMGPVIASIHAKR
DEFRDKIQRDAGLAERPKLRTSSIQG
>Mature_585_residues
AGELSPSPILQDALVILGAAGIVIPVFTRFKITPVIGFILIGVLVGPFGLGAWVYEYDWLEYITISDPEGLDPFAEFGII
LLLFAIGLELSFKRLWTLRKLVFGLGAMELLVIGTCAAAVLAMMGQYWVGALALGFALAFSSTAIVLPISGTKTPVGRAA
LSMLLFEDIMIVPIVFILGALAPFAQSDGVTGLVDTLWQGGLVIVALLVLGRIALPRLFAQAARTKSPELFLAASLLVVI
GSSLATGVTGLSPIVGALIAGLLIAETEYHGEVESIMEPFKGLALGIFLITVGMSIDLASIWDNLWPILTAVVLVLVFKA
IVTGTLLRLMGARRSTAAETGVLMASPSETTLIVLSAAGSAMLIQPGTQQFWQIVTAIGLTITPLLAMLGRVIARRVEPN
PSLDVGESDEPRVIIIGVGRVGMLVADMLEAHERPYVAIDSNADLIARARRRGYRAVYGDAARGDALDKLGVENSPAVVL
TMDEPVLTDRLTSKLRKAYPDLLIVTRARDSQHAADLYRAGASHAVPETLESSLQLSEAVLADIGVPMGPVIASIHAKRD
EFRDKIQRDAGLAERPKLRTSSIQG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=568, Percent_Identity=33.6267605633803, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789749, Length=571, Percent_Identity=31.8739054290718, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1786685, Length=521, Percent_Identity=26.1036468330134, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62268; Mature: 62137

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGELSPSPILQDALVILGAAGIVIPVFTRFKITPVIGFILIGVLVGPFGLGAWVYEYDW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECC
LEYITISDPEGLDPFAEFGIILLLFAIGLELSFKRLWTLRKLVFGLGAMELLVIGTCAAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VLAMMGQYWVGALALGFALAFSSTAIVLPISGTKTPVGRAALSMLLFEDIMIVPIVFILG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAPFAQSDGVTGLVDTLWQGGLVIVALLVLGRIALPRLFAQAARTKSPELFLAASLLVV
HHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IGSSLATGVTGLSPIVGALIAGLLIAETEYHGEVESIMEPFKGLALGIFLITVGMSIDLA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SIWDNLWPILTAVVLVLVFKAIVTGTLLRLMGARRSTAAETGVLMASPSETTLIVLSAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCEEEEEECCC
SAMLIQPGTQQFWQIVTAIGLTITPLLAMLGRVIARRVEPNPSLDVGESDEPRVIIIGVG
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
RVGMLVADMLEAHERPYVAIDSNADLIARARRRGYRAVYGDAARGDALDKLGVENSPAVV
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCCEEE
LTMDEPVLTDRLTSKLRKAYPDLLIVTRARDSQHAADLYRAGASHAVPETLESSLQLSEA
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VLADIGVPMGPVIASIHAKRDEFRDKIQRDAGLAERPKLRTSSIQG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGELSPSPILQDALVILGAAGIVIPVFTRFKITPVIGFILIGVLVGPFGLGAWVYEYDW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECC
LEYITISDPEGLDPFAEFGIILLLFAIGLELSFKRLWTLRKLVFGLGAMELLVIGTCAAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VLAMMGQYWVGALALGFALAFSSTAIVLPISGTKTPVGRAALSMLLFEDIMIVPIVFILG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAPFAQSDGVTGLVDTLWQGGLVIVALLVLGRIALPRLFAQAARTKSPELFLAASLLVV
HHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IGSSLATGVTGLSPIVGALIAGLLIAETEYHGEVESIMEPFKGLALGIFLITVGMSIDLA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SIWDNLWPILTAVVLVLVFKAIVTGTLLRLMGARRSTAAETGVLMASPSETTLIVLSAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCEEEEEECCC
SAMLIQPGTQQFWQIVTAIGLTITPLLAMLGRVIARRVEPNPSLDVGESDEPRVIIIGVG
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
RVGMLVADMLEAHERPYVAIDSNADLIARARRRGYRAVYGDAARGDALDKLGVENSPAVV
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCCEEE
LTMDEPVLTDRLTSKLRKAYPDLLIVTRARDSQHAADLYRAGASHAVPETLESSLQLSEA
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VLADIGVPMGPVIASIHAKRDEFRDKIQRDAGLAERPKLRTSSIQG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA