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The map label for this gene is fgs [H]

Identifier: 85374348

GI number: 85374348

Start: 1573829

End: 1575118

Strand: Direct

Name: fgs [H]

Synonym: ELI_07605

Alternate gene names: 85374348

Gene position: 1573829-1575118 (Clockwise)

Preceding gene: 85374347

Following gene: 85374350

Centisome position: 51.56

GC content: 64.57

Gene sequence:

>1290_bases
ATGGACTTCGCACGCTCCTCCCACCCCGGCGTGCAGGCCCAGCTCGACCGGCTCGGCCTGCTGAGCGTCCCCGACGGGCG
GTTCGGGCTGGAAACGATCACCACCCTGCTCGACCGGCTCGGCAATCCGCATCGCCAGCTACCGCCGACGTTCCATGTCG
CGGGGACCAACGGCAAAGGCTCGGTCTGCGCCTTCCTGCGCGCCATGCTGGAGGCGGATGGCAAGCGCGTCCACGTCACC
ACCAGCCCGCACCTCGTGCGCTACAATGAACGTATCCTGCTGGCTGGCAGACTGATCGAAGACGAGCCGCTGGCGGCGCT
GCTGGAAGAGGTGCTCGATGCGAGCGAAGAGTTGGCCCCCAGCTTTTTCGAAGTCACCATCGCCGCCGCCTTCCTCGCAT
TCAGCCGCACACCTGCCGATGCCTGCGTAGTTGAGGTCGGCCTTGGCGGGAGATTCGACGCGACCAATGTGCTGCAATCG
GACGTGCTGGCGGCTTGCGGAATCGCGGCGCTGGGGATCGATCACGAACGCTTCCTCCTCGCCCCGGAGGACGGCGTGCC
GAAAGACCCGATGGCGCGGATCGCATTTGAGAAGGCGGGGATCGCGAAGAAAGGCATACCGCTGGTGACCGGCTTCCAAC
AAGCAGGTCCGGCCTTGGAAATCGAACGGGCGATATTGCGCAGCGAGGCCACGCTGAAAATGCGCGGTCGTGAATGGCAC
GCGAGCATGGACGACGCCGGGCTGCACTACGCCGATTTCGGCGGCGCTCTAGACTTGCCGCAGCCATCCATGCCCGGCGG
ACATCAAATCGATAATGCGGCGCTGGCTGTCGCGATGCTGCGTCACCAAGAAGCGTTGTCGGTCTCGCCCGAGGCGATGG
CGCAAGGGCTGCGCGCGGCGCATTGGCCTGCACGGATGCAGCGGCTGAAACCGGGCCCACTCACCGGGGATCGGGCCGTC
TGGCTCGATGGCGGACACAATCCCAGCGCGGGTGCGACGATTGCTGCGTTTTTCGAGGGGCCGGTTCATCTTGTTCTGGG
TATGCTCGACAACAAGGACCCGCAGGCGCTGATTGCGCCACTTGGAGACCGCATTCGTAGCCTTACCGTAGTGCCGGTGC
CGGGGCATGACAGCCATCCGGTCGAGGCTTTCGGCCCCGATGCCAAGGCTGTTGATACTATCGAAGAGGCGCTGCTTGGC
ATCCCCGCAGATGACTACCCGATCTTGATCGCAGGCTCGCTCTACCTTGCGGGGGAGGTGTTGCGACTTAATGACGGGAT
CCCCAACTAG

Upstream 100 bases:

>100_bases
TAATGAACATCGTCATCCCAGCGAAAGCTGGGATCTCGCTCCACCAGGATCGACGCAAGTGGCGAGAGACCCCAGCTTTC
GCTGGGGTGACGAGGTAGCC

Downstream 100 bases:

>100_bases
CTAACCAAGTCCGTTCGGGCTGAGCCTGTCGAATCCCAGTCACGGCGTTGTGCCAAGACCAAAGGCCCGGGGCTGCCCTG
CGACAGGCTCAGGGCGAACG

Product: putative folylpolyglutamate/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MDFARSSHPGVQAQLDRLGLLSVPDGRFGLETITTLLDRLGNPHRQLPPTFHVAGTNGKGSVCAFLRAMLEADGKRVHVT
TSPHLVRYNERILLAGRLIEDEPLAALLEEVLDASEELAPSFFEVTIAAAFLAFSRTPADACVVEVGLGGRFDATNVLQS
DVLAACGIAALGIDHERFLLAPEDGVPKDPMARIAFEKAGIAKKGIPLVTGFQQAGPALEIERAILRSEATLKMRGREWH
ASMDDAGLHYADFGGALDLPQPSMPGGHQIDNAALAVAMLRHQEALSVSPEAMAQGLRAAHWPARMQRLKPGPLTGDRAV
WLDGGHNPSAGATIAAFFEGPVHLVLGMLDNKDPQALIAPLGDRIRSLTVVPVPGHDSHPVEAFGPDAKAVDTIEEALLG
IPADDYPILIAGSLYLAGEVLRLNDGIPN

Sequences:

>Translated_429_residues
MDFARSSHPGVQAQLDRLGLLSVPDGRFGLETITTLLDRLGNPHRQLPPTFHVAGTNGKGSVCAFLRAMLEADGKRVHVT
TSPHLVRYNERILLAGRLIEDEPLAALLEEVLDASEELAPSFFEVTIAAAFLAFSRTPADACVVEVGLGGRFDATNVLQS
DVLAACGIAALGIDHERFLLAPEDGVPKDPMARIAFEKAGIAKKGIPLVTGFQQAGPALEIERAILRSEATLKMRGREWH
ASMDDAGLHYADFGGALDLPQPSMPGGHQIDNAALAVAMLRHQEALSVSPEAMAQGLRAAHWPARMQRLKPGPLTGDRAV
WLDGGHNPSAGATIAAFFEGPVHLVLGMLDNKDPQALIAPLGDRIRSLTVVPVPGHDSHPVEAFGPDAKAVDTIEEALLG
IPADDYPILIAGSLYLAGEVLRLNDGIPN
>Mature_429_residues
MDFARSSHPGVQAQLDRLGLLSVPDGRFGLETITTLLDRLGNPHRQLPPTFHVAGTNGKGSVCAFLRAMLEADGKRVHVT
TSPHLVRYNERILLAGRLIEDEPLAALLEEVLDASEELAPSFFEVTIAAAFLAFSRTPADACVVEVGLGGRFDATNVLQS
DVLAACGIAALGIDHERFLLAPEDGVPKDPMARIAFEKAGIAKKGIPLVTGFQQAGPALEIERAILRSEATLKMRGREWH
ASMDDAGLHYADFGGALDLPQPSMPGGHQIDNAALAVAMLRHQEALSVSPEAMAQGLRAAHWPARMQRLKPGPLTGDRAV
WLDGGHNPSAGATIAAFFEGPVHLVLGMLDNKDPQALIAPLGDRIRSLTVVPVPGHDSHPVEAFGPDAKAVDTIEEALLG
IPADDYPILIAGSLYLAGEVLRLNDGIPN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=334, Percent_Identity=33.2335329341317, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI66932984, Length=367, Percent_Identity=31.6076294277929, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1788654, Length=413, Percent_Identity=30.9927360774818, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71984923, Length=342, Percent_Identity=26.6081871345029, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17553148, Length=342, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=342, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=29.7235023041475, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=347, Percent_Identity=28.2420749279539, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322718, Length=454, Percent_Identity=24.6696035242291, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641571, Length=326, Percent_Identity=26.6871165644172, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24581568, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45612; Mature: 45612

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFARSSHPGVQAQLDRLGLLSVPDGRFGLETITTLLDRLGNPHRQLPPTFHVAGTNGKG
CCCCCCCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
SVCAFLRAMLEADGKRVHVTTSPHLVRYNERILLAGRLIEDEPLAALLEEVLDASEELAP
HHHHHHHHHHHCCCCEEEEECCCCEEEECCEEEEECCCCCCCHHHHHHHHHHCCHHHHCC
SFFEVTIAAAFLAFSRTPADACVVEVGLGGRFDATNVLQSDVLAACGIAALGIDHERFLL
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
APEDGVPKDPMARIAFEKAGIAKKGIPLVTGFQQAGPALEIERAILRSEATLKMRGREWH
CCCCCCCCCHHHHHHHHHCCCCCCCCCEEECHHHCCCHHHHHHHHHHCCCEEEECCCCCC
ASMDDAGLHYADFGGALDLPQPSMPGGHQIDNAALAVAMLRHQEALSVSPEAMAQGLRAA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HWPARMQRLKPGPLTGDRAVWLDGGHNPSAGATIAAFFEGPVHLVLGMLDNKDPQALIAP
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHH
LGDRIRSLTVVPVPGHDSHPVEAFGPDAKAVDTIEEALLGIPADDYPILIAGSLYLAGEV
HHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCEE
LRLNDGIPN
EEECCCCCC
>Mature Secondary Structure
MDFARSSHPGVQAQLDRLGLLSVPDGRFGLETITTLLDRLGNPHRQLPPTFHVAGTNGKG
CCCCCCCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
SVCAFLRAMLEADGKRVHVTTSPHLVRYNERILLAGRLIEDEPLAALLEEVLDASEELAP
HHHHHHHHHHHCCCCEEEEECCCCEEEECCEEEEECCCCCCCHHHHHHHHHHCCHHHHCC
SFFEVTIAAAFLAFSRTPADACVVEVGLGGRFDATNVLQSDVLAACGIAALGIDHERFLL
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
APEDGVPKDPMARIAFEKAGIAKKGIPLVTGFQQAGPALEIERAILRSEATLKMRGREWH
CCCCCCCCCHHHHHHHHHCCCCCCCCCEEECHHHCCCHHHHHHHHHHCCCEEEECCCCCC
ASMDDAGLHYADFGGALDLPQPSMPGGHQIDNAALAVAMLRHQEALSVSPEAMAQGLRAA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HWPARMQRLKPGPLTGDRAVWLDGGHNPSAGATIAAFFEGPVHLVLGMLDNKDPQALIAP
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHH
LGDRIRSLTVVPVPGHDSHPVEAFGPDAKAVDTIEEALLGIPADDYPILIAGSLYLAGEV
HHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCEE
LRLNDGIPN
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105929; 9618466 [H]