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The map label for this gene is aroA [H]

Identifier: 85374325

GI number: 85374325

Start: 1552549

End: 1553940

Strand: Direct

Name: aroA [H]

Synonym: ELI_07490

Alternate gene names: 85374325

Gene position: 1552549-1553940 (Clockwise)

Preceding gene: 85374314

Following gene: 85374326

Centisome position: 50.86

GC content: 67.6

Gene sequence:

>1392_bases
ATGGCGCTGTCAAAAGCCGCGCCCGTGACAAATGCCGTGACAGACGCCCCGATCCCGCATCGTTTTACCGCCCCGGGCCC
GCTCACGGGGCGCATCCGCGTGCCCGGCGACAAGTCGATCAGCCATCGCTCGATCATGCTCGGCGCGCTGGCGATCGGCG
AGACGCGCGTCACCGGCCTGCTCGAAGGCGAAGACGTGATGGCGACCGCCGCAGCGATGCGGGCAATGGGCGCGCAGGTC
CTTCGACAGGCTCAGGATGGTCGGGCTGATGCCGAATGGCGCGTGCATGGCGTCGGCGTCGGCGGGTTGCTCCAGCCGCG
CACCGCGCTCGATATGGGCAATAGCGGCACTTCGACACGGCTGCTGATGGGCCTCGTCGCCAGCCACGGCATCACCGCGA
CCTTTATCGGCGATGCGAGCCTCTCGGGACGCCCGATGAGCCGCGTGACAGAGCCGCTCGGCCGGATGGGTGCCCAGTTT
CACGGTGCTTCGGGCGGAACCTTGCCAATCACCATGGAAGGCCTCTGCCCCGCCGTGCCGATCGAGTACCGGCTGCCGGT
CGCCAGCGCGCAGGTGAAGAGCGCGGTGATGCTCGCCGCACTCAACACGCCGGGCATCACGACCGTGATCGAGCCGGTGC
CGACGCGCGATCATTCGGAGCGGATGCTCAAGGGCTTCGGTGCGGATGTCGAAATCGAGGAGGTGGATGGCGCCACAGGG
CGGGAACGCGCGATCCGCGTGCGCGGCGAGGCGGACCTGCAGCCGCAGATTATCGAGGTGCCGGGCGATCCCTCCTCCGC
CGCCTTCTTCGTCGTCGCGGCGCTGCTGGTCGAAGGCAGCGATCTCGTGATCGAAAATGTCGGTCTCAACCCGACCCGTG
CGGGCCTGTTCGAAGTGCTGCGCCAGATGGGCGGGCACATCGAGGAGATGAACACCCGCGAGGTCGGCGGAGAGCCGGTC
GCGGACCTGCGGGTCAGGCATTCGGCGCTGACCGGCATCGAGGTCGATCCAGCCATCGCGCCGAGCATGATCGACGAATT
CCCCGTGCTGTTCGTCGCCGCTTCGCTGGCCGAAGGCACGACCACCACCAGCGGTCTCGAAGAATTGCGCGTCAAGGAAA
GCGACCGTCTTGCCGCGATGGCCGCTGCCCTGACTGGTGCAGGCGCGACCATCGAAGAGCGAGAGGATGGCCTGGTGATC
CATGGCACCGGGGGCGCACCCCTGCGCGGCTCTGCCAACAGCGCTACCCAAACGCTGTTAGACCACCGCATCGCCATGAG
CATGGCCGTGGCCGGCCTCGCCAGCCGTGACGGTGTCGAGGTGGACGATACGCGCCCCATCGCCACGAGCTTTCCGAACT
TTATGGCGTTACTGGGGGGGGCGGTGCGATGA

Upstream 100 bases:

>100_bases
GTCGGGGCGGCAGGATCGCCGGACACCCGCGAAAATTCTCTATCTTGATGGGTAATAGAAGGCCCCGAATCAAGAGCGCG
GCGCGCCTTGCCATAGGGGC

Downstream 100 bases:

>100_bases
CCTCCTATGTCTCCATCGCCCCGGACTTGATCCGGGGTCCCGCTCCTTCTGGCCTTGGACGAAAGGCAGCGGGATTCCGG
CTCGGGGGCCGGGATGACGA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MALSKAAPVTNAVTDAPIPHRFTAPGPLTGRIRVPGDKSISHRSIMLGALAIGETRVTGLLEGEDVMATAAAMRAMGAQV
LRQAQDGRADAEWRVHGVGVGGLLQPRTALDMGNSGTSTRLLMGLVASHGITATFIGDASLSGRPMSRVTEPLGRMGAQF
HGASGGTLPITMEGLCPAVPIEYRLPVASAQVKSAVMLAALNTPGITTVIEPVPTRDHSERMLKGFGADVEIEEVDGATG
RERAIRVRGEADLQPQIIEVPGDPSSAAFFVVAALLVEGSDLVIENVGLNPTRAGLFEVLRQMGGHIEEMNTREVGGEPV
ADLRVRHSALTGIEVDPAIAPSMIDEFPVLFVAASLAEGTTTTSGLEELRVKESDRLAAMAAALTGAGATIEEREDGLVI
HGTGGAPLRGSANSATQTLLDHRIAMSMAVAGLASRDGVEVDDTRPIATSFPNFMALLGGAVR

Sequences:

>Translated_463_residues
MALSKAAPVTNAVTDAPIPHRFTAPGPLTGRIRVPGDKSISHRSIMLGALAIGETRVTGLLEGEDVMATAAAMRAMGAQV
LRQAQDGRADAEWRVHGVGVGGLLQPRTALDMGNSGTSTRLLMGLVASHGITATFIGDASLSGRPMSRVTEPLGRMGAQF
HGASGGTLPITMEGLCPAVPIEYRLPVASAQVKSAVMLAALNTPGITTVIEPVPTRDHSERMLKGFGADVEIEEVDGATG
RERAIRVRGEADLQPQIIEVPGDPSSAAFFVVAALLVEGSDLVIENVGLNPTRAGLFEVLRQMGGHIEEMNTREVGGEPV
ADLRVRHSALTGIEVDPAIAPSMIDEFPVLFVAASLAEGTTTTSGLEELRVKESDRLAAMAAALTGAGATIEEREDGLVI
HGTGGAPLRGSANSATQTLLDHRIAMSMAVAGLASRDGVEVDDTRPIATSFPNFMALLGGAVR
>Mature_462_residues
ALSKAAPVTNAVTDAPIPHRFTAPGPLTGRIRVPGDKSISHRSIMLGALAIGETRVTGLLEGEDVMATAAAMRAMGAQVL
RQAQDGRADAEWRVHGVGVGGLLQPRTALDMGNSGTSTRLLMGLVASHGITATFIGDASLSGRPMSRVTEPLGRMGAQFH
GASGGTLPITMEGLCPAVPIEYRLPVASAQVKSAVMLAALNTPGITTVIEPVPTRDHSERMLKGFGADVEIEEVDGATGR
ERAIRVRGEADLQPQIIEVPGDPSSAAFFVVAALLVEGSDLVIENVGLNPTRAGLFEVLRQMGGHIEEMNTREVGGEPVA
DLRVRHSALTGIEVDPAIAPSMIDEFPVLFVAASLAEGTTTTSGLEELRVKESDRLAAMAAALTGAGATIEEREDGLVIH
GTGGAPLRGSANSATQTLLDHRIAMSMAVAGLASRDGVEVDDTRPIATSFPNFMALLGGAVR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=439, Percent_Identity=27.7904328018223, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320332, Length=443, Percent_Identity=25.9593679458239, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 48053; Mature: 47921

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSKAAPVTNAVTDAPIPHRFTAPGPLTGRIRVPGDKSISHRSIMLGALAIGETRVTGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEECCHHEEEE
LEGEDVMATAAAMRAMGAQVLRQAQDGRADAEWRVHGVGVGGLLQPRTALDMGNSGTSTR
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
LLMGLVASHGITATFIGDASLSGRPMSRVTEPLGRMGAQFHGASGGTLPITMEGLCPAVP
HHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCC
IEYRLPVASAQVKSAVMLAALNTPGITTVIEPVPTRDHSERMLKGFGADVEIEEVDGATG
CEEECCCHHHHHHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCC
RERAIRVRGEADLQPQIIEVPGDPSSAAFFVVAALLVEGSDLVIENVGLNPTRAGLFEVL
CCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
RQMGGHIEEMNTREVGGEPVADLRVRHSALTGIEVDPAIAPSMIDEFPVLFVAASLAEGT
HHHCCCHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
TTTSGLEELRVKESDRLAAMAAALTGAGATIEEREDGLVIHGTGGAPLRGSANSATQTLL
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHH
DHRIAMSMAVAGLASRDGVEVDDTRPIATSFPNFMALLGGAVR
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
ALSKAAPVTNAVTDAPIPHRFTAPGPLTGRIRVPGDKSISHRSIMLGALAIGETRVTGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEECCHHEEEE
LEGEDVMATAAAMRAMGAQVLRQAQDGRADAEWRVHGVGVGGLLQPRTALDMGNSGTSTR
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
LLMGLVASHGITATFIGDASLSGRPMSRVTEPLGRMGAQFHGASGGTLPITMEGLCPAVP
HHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCC
IEYRLPVASAQVKSAVMLAALNTPGITTVIEPVPTRDHSERMLKGFGADVEIEEVDGATG
CEEECCCHHHHHHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCC
RERAIRVRGEADLQPQIIEVPGDPSSAAFFVVAALLVEGSDLVIENVGLNPTRAGLFEVL
CCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
RQMGGHIEEMNTREVGGEPVADLRVRHSALTGIEVDPAIAPSMIDEFPVLFVAASLAEGT
HHHCCCHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
TTTSGLEELRVKESDRLAAMAAALTGAGATIEEREDGLVIHGTGGAPLRGSANSATQTLL
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHH
DHRIAMSMAVAGLASRDGVEVDDTRPIATSFPNFMALLGGAVR
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA