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The map label for this gene is proA

Identifier: 85374275

GI number: 85374275

Start: 1497499

End: 1498764

Strand: Direct

Name: proA

Synonym: ELI_07240

Alternate gene names: 85374275

Gene position: 1497499-1498764 (Clockwise)

Preceding gene: 85374267

Following gene: 85374276

Centisome position: 49.06

GC content: 67.69

Gene sequence:

>1266_bases
ATGAACGACCAGACACTCGATCCGGCGATCCACATCCAGCAACTCGGCACCGCAGCGCGCGACGCTGCGCGCGGGCTCGT
CTCCGCCAGCACCGAAGCGAAGAATAAAGCGCTGATGGAAGCGGCCAGGGCGCTGCGCGAAGCGACGCCGGCGCTGCTCG
AAGCCAACGCCAGGGATGTGGCGAGCGTCGAGGGCAAGAAGGACGAAGCGTTTATCGACCGGCTCAAGCTGGACGAAGCC
CGCGTCGAAGGCATGGCGAGCGCACTCGAACAGATCGCCGAACTGCCCGATCCCGTCGGCCGTGTGCTCGCCCAGTTCGA
CCGGCCCAACGGCCTCAGGATCGAGCGGGTGGCGGTGCCGATCGGCGTGATCGGCATGATCTACGAAAGCCGCCCCAATG
TCGGCGCCGATGCCAGCGCCCTGTGCCTCAAGAGCGGTAACGCCGTGATCCTGCGCGGGGGCAGCGAGAGCCGCCATTCG
ACGCGCGAGATCGTCGCCTGCATGCAGGCCGGGCTCAAGGCGGCGGGCCTGCCCGAGAACGCCGTACAGACCGTCCAGAC
GACCGACCGCAATGCCGTCGCCGAGCTGCTCAAGGCCGACGAACACGTCGACCTCGTCATTCCGCGAGGCGGACGCGGGC
TCGTCGAGCTGGTGCGCGACCAGGCGAGCGTGCCGACGCTGCTGCACCTCGACGGCAATTGCCACTCCTATGTCCACGAA
GCGGCCGATGTCGCCAAGGCCGTCGACGTCGTCCGCAATGCCAAGCTGCGCCGCACCGGCATCTGCGGGGCGACCGAGAG
CGTCGTCGTCGACCGCGTCATCGCCCCCGCCCTCATCCTCGCCCTCGCCGATGCGATGGCCGGTGACTGCGAGCTGCGCG
GCGACGAGACTGCGGTACAGCTCGACGACCGCATCACCCCGGCCTCCGAGGACGACTGGAACACCGAATATCTCGGCCCC
ATCGCCAGCGTGAAGGTGGTCGACGGGCTCGACGAAGCGATCGAATGGGTCGAAGGCCACAGCTCGCACCACACCGATGC
GATCCTGACCGAAGACGCCGAGGCCGCACGCCGCTTCATGACCGCAATCGACAGCGCCATCGTGATGCACAACGCCTCCA
CGCAATTTGCCGACGGCGGCGAGTTCGGCATGGGCGCGGAGATCGGCATCGCCACCGGCAAGATGCACGCCCGCGGCCCC
GTGGGGCTGGAGCAGCTGACCAGCTTCAAGTACCTCGTGCATGGCTCAGGGCAGACTCGCGAATAA

Upstream 100 bases:

>100_bases
TACCTATGTTGCAATGCAGCAACTTCAACGGTTTTCTGCGCCGGGCACTCTTCGTGTGCCGTCCGCCGCGCGGGGCCGAC
AGACGATACAGGGAGCACCC

Downstream 100 bases:

>100_bases
TTTTCCTACACGCGCATTTTCTGCTTCACGCTTGCGGATGACCCGCCGCACCGACAACCGCACGACCCGCTTGCCCGTCC
CGGCAGGCGAAATCCCCACC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNDQTLDPAIHIQQLGTAARDAARGLVSASTEAKNKALMEAARALREATPALLEANARDVASVEGKKDEAFIDRLKLDEA
RVEGMASALEQIAELPDPVGRVLAQFDRPNGLRIERVAVPIGVIGMIYESRPNVGADASALCLKSGNAVILRGGSESRHS
TREIVACMQAGLKAAGLPENAVQTVQTTDRNAVAELLKADEHVDLVIPRGGRGLVELVRDQASVPTLLHLDGNCHSYVHE
AADVAKAVDVVRNAKLRRTGICGATESVVVDRVIAPALILALADAMAGDCELRGDETAVQLDDRITPASEDDWNTEYLGP
IASVKVVDGLDEAIEWVEGHSSHHTDAILTEDAEAARRFMTAIDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGLEQLTSFKYLVHGSGQTRE

Sequences:

>Translated_421_residues
MNDQTLDPAIHIQQLGTAARDAARGLVSASTEAKNKALMEAARALREATPALLEANARDVASVEGKKDEAFIDRLKLDEA
RVEGMASALEQIAELPDPVGRVLAQFDRPNGLRIERVAVPIGVIGMIYESRPNVGADASALCLKSGNAVILRGGSESRHS
TREIVACMQAGLKAAGLPENAVQTVQTTDRNAVAELLKADEHVDLVIPRGGRGLVELVRDQASVPTLLHLDGNCHSYVHE
AADVAKAVDVVRNAKLRRTGICGATESVVVDRVIAPALILALADAMAGDCELRGDETAVQLDDRITPASEDDWNTEYLGP
IASVKVVDGLDEAIEWVEGHSSHHTDAILTEDAEAARRFMTAIDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGLEQLTSFKYLVHGSGQTRE
>Mature_421_residues
MNDQTLDPAIHIQQLGTAARDAARGLVSASTEAKNKALMEAARALREATPALLEANARDVASVEGKKDEAFIDRLKLDEA
RVEGMASALEQIAELPDPVGRVLAQFDRPNGLRIERVAVPIGVIGMIYESRPNVGADASALCLKSGNAVILRGGSESRHS
TREIVACMQAGLKAAGLPENAVQTVQTTDRNAVAELLKADEHVDLVIPRGGRGLVELVRDQASVPTLLHLDGNCHSYVHE
AADVAKAVDVVRNAKLRRTGICGATESVVVDRVIAPALILALADAMAGDCELRGDETAVQLDDRITPASEDDWNTEYLGP
IASVKVVDGLDEAIEWVEGHSSHHTDAILTEDAEAARRFMTAIDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGLEQLTSFKYLVHGSGQTRE

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=35.8353510895884, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=35.8353510895884, Blast_Score=239, Evalue=4e-63,
Organism=Escherichia coli, GI1786438, Length=423, Percent_Identity=41.1347517730496, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17569969, Length=417, Percent_Identity=34.2925659472422, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17569967, Length=417, Percent_Identity=34.2925659472422, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=36.214953271028, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI21357643, Length=420, Percent_Identity=35, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_ERYLH (Q2N9V1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458337.1
- HSSP:   P54886
- ProteinModelPortal:   Q2N9V1
- SMR:   Q2N9V1
- STRING:   Q2N9V1
- GeneID:   3871837
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07240
- NMPDR:   fig|314225.3.peg.1599
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   GICGAME
- PhylomeDB:   Q2N9V1
- ProtClustDB:   PRK00197
- BioCyc:   ELIT314225:ELI_07240-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44703; Mature: 44703

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQTLDPAIHIQQLGTAARDAARGLVSASTEAKNKALMEAARALREATPALLEANARDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHH
ASVEGKKDEAFIDRLKLDEARVEGMASALEQIAELPDPVGRVLAQFDRPNGLRIERVAVP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECC
IGVIGMIYESRPNVGADASALCLKSGNAVILRGGSESRHSTREIVACMQAGLKAAGLPEN
HHHHHHHHCCCCCCCCCCHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHH
AVQTVQTTDRNAVAELLKADEHVDLVIPRGGRGLVELVRDQASVPTLLHLDGNCHSYVHE
HHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
AADVAKAVDVVRNAKLRRTGICGATESVVVDRVIAPALILALADAMAGDCELRGDETAVQ
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
LDDRITPASEDDWNTEYLGPIASVKVVDGLDEAIEWVEGHSSHHTDAILTEDAEAARRFM
ECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHH
TAIDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGPVGLEQLTSFKYLVHGSGQTR
HHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHEEEECCCCCC
E
C
>Mature Secondary Structure
MNDQTLDPAIHIQQLGTAARDAARGLVSASTEAKNKALMEAARALREATPALLEANARDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHH
ASVEGKKDEAFIDRLKLDEARVEGMASALEQIAELPDPVGRVLAQFDRPNGLRIERVAVP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECC
IGVIGMIYESRPNVGADASALCLKSGNAVILRGGSESRHSTREIVACMQAGLKAAGLPEN
HHHHHHHHCCCCCCCCCCHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHH
AVQTVQTTDRNAVAELLKADEHVDLVIPRGGRGLVELVRDQASVPTLLHLDGNCHSYVHE
HHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
AADVAKAVDVVRNAKLRRTGICGATESVVVDRVIAPALILALADAMAGDCELRGDETAVQ
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
LDDRITPASEDDWNTEYLGPIASVKVVDGLDEAIEWVEGHSSHHTDAILTEDAEAARRFM
ECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHH
TAIDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGPVGLEQLTSFKYLVHGSGQTR
HHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHEEEECCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA