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The map label for this gene is echA12 [H]

Identifier: 85374265

GI number: 85374265

Start: 1486742

End: 1487545

Strand: Direct

Name: echA12 [H]

Synonym: ELI_07190

Alternate gene names: 85374265

Gene position: 1486742-1487545 (Clockwise)

Preceding gene: 85374264

Following gene: 85374266

Centisome position: 48.71

GC content: 63.18

Gene sequence:

>804_bases
ATGCACAATGACTACACCACCATCACCGTCGAAAAGCGCGGCGAGGTCGACTGGCTGACGCTCGATCGGCCCGATGCGCT
CAATGCCATCACGCTCGACATGGTGGCGGAGCTCAACGACTATTTCGGACGGCTCTACAACGACGGGTCGGTGCGCGTGG
TGGTGATGCGCGGTGCGGGCAAGGCCTATTGCGCCGGGCTCGACATCAAGGAGCGGCAGGGCGAAGATCAGGAATTCCCC
TTCGGTGGCGGGTTCGGATTCCAGGGCTTTCTCGCCGATGTCTATGTGAAGATGCGCCGTTGCCCGCAGGTGATCCTCTC
GCTGGTGCACGGACCGGCATGCGGCGGCGGGTTCGCTTTCGCCCTCGCCTCCGATATTCGGCTGGCGGGTGAGAGCGCCC
GCATGAATGCCGCTTTCATCAAGATCGGCCTGTCCTCATGCGACATGGGTGTGAGCTATTTCCTGCCGCGCCTGGTCGGG
CAATCCATTGCCAGCGAGCTGATGCTGACCGGGCGTTTCATCCATGCCGAGCGTGCGTTGGCCGTAGGGCTGGTTTCCGA
AGTCGTGCCGGACGACCAGTTGGAGAGCGCAGCGCAAGGCTGGATCGACGACCTGCTCGCCGCCTCGCCAAAGGGCCTGC
GCATGACCAAGGAAGGGCTGCAGATCGCCACCGATGCCGGCAGCCTCGAAGCCGCCATGGCGATCGAGAACCGCAACCAG
CTGATGACCAGCGCCAGCCCCAATTTTCATGAAGGCATGCGCGCCTTCCTCGAAAAGCGGAAGCCCGACTACACGCCCGA
CTAA

Upstream 100 bases:

>100_bases
AAGCGGGAATCAAGATCGGCACCTGGACACCGCTCGCGGGCGGCGTGATGAAGTGGATACCCGAGGGTGAATAGCCAGCC
AAAACTACGAGGGGCCCGCC

Downstream 100 bases:

>100_bases
TGCGGAACATCGCAGCGCGGAGCGCTTTGATCAAGCATCAGCCCCAACAGCAGAGCGAGATTTCCCATGGCCAAGACCCT
CCTCATCACCGGCGCATCGA

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MHNDYTTITVEKRGEVDWLTLDRPDALNAITLDMVAELNDYFGRLYNDGSVRVVVMRGAGKAYCAGLDIKERQGEDQEFP
FGGGFGFQGFLADVYVKMRRCPQVILSLVHGPACGGGFAFALASDIRLAGESARMNAAFIKIGLSSCDMGVSYFLPRLVG
QSIASELMLTGRFIHAERALAVGLVSEVVPDDQLESAAQGWIDDLLAASPKGLRMTKEGLQIATDAGSLEAAMAIENRNQ
LMTSASPNFHEGMRAFLEKRKPDYTPD

Sequences:

>Translated_267_residues
MHNDYTTITVEKRGEVDWLTLDRPDALNAITLDMVAELNDYFGRLYNDGSVRVVVMRGAGKAYCAGLDIKERQGEDQEFP
FGGGFGFQGFLADVYVKMRRCPQVILSLVHGPACGGGFAFALASDIRLAGESARMNAAFIKIGLSSCDMGVSYFLPRLVG
QSIASELMLTGRFIHAERALAVGLVSEVVPDDQLESAAQGWIDDLLAASPKGLRMTKEGLQIATDAGSLEAAMAIENRNQ
LMTSASPNFHEGMRAFLEKRKPDYTPD
>Mature_267_residues
MHNDYTTITVEKRGEVDWLTLDRPDALNAITLDMVAELNDYFGRLYNDGSVRVVVMRGAGKAYCAGLDIKERQGEDQEFP
FGGGFGFQGFLADVYVKMRRCPQVILSLVHGPACGGGFAFALASDIRLAGESARMNAAFIKIGLSSCDMGVSYFLPRLVG
QSIASELMLTGRFIHAERALAVGLVSEVVPDDQLESAAQGWIDDLLAASPKGLRMTKEGLQIATDAGSLEAAMAIENRNQ
LMTSASPNFHEGMRAFLEKRKPDYTPD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=268, Percent_Identity=32.4626865671642, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=30.3418803418803, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=25.7936507936508, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=30.6513409961686, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI68989263, Length=175, Percent_Identity=28, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI62530384, Length=244, Percent_Identity=23.7704918032787, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=209, Percent_Identity=24.8803827751196, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI157694516, Length=209, Percent_Identity=24.8803827751196, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787660, Length=247, Percent_Identity=31.5789473684211, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=30.6818181818182, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=31.2, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=30.9433962264151, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=27.6119402985075, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790281, Length=197, Percent_Identity=27.9187817258883, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=27.6595744680851, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=34.2307692307692, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=33.7164750957854, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=27.9527559055118, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=30.7339449541284, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=25.8928571428571, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=208, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=198, Percent_Identity=28.2828282828283, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=25.6302521008403, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20129971, Length=269, Percent_Identity=33.0855018587361, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24653477, Length=269, Percent_Identity=33.0855018587361, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24653139, Length=275, Percent_Identity=30.9090909090909, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=30.952380952381, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=24.4274809160305, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=198, Percent_Identity=26.2626262626263, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=183, Percent_Identity=27.3224043715847, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29037; Mature: 29037

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNDYTTITVEKRGEVDWLTLDRPDALNAITLDMVAELNDYFGRLYNDGSVRVVVMRGAG
CCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
KAYCAGLDIKERQGEDQEFPFGGGFGFQGFLADVYVKMRRCPQVILSLVHGPACGGGFAF
CEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ALASDIRLAGESARMNAAFIKIGLSSCDMGVSYFLPRLVGQSIASELMLTGRFIHAERAL
HHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGLVSEVVPDDQLESAAQGWIDDLLAASPKGLRMTKEGLQIATDAGSLEAAMAIENRNQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEECCCCHHHHHHHHCCHH
LMTSASPNFHEGMRAFLEKRKPDYTPD
HHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHNDYTTITVEKRGEVDWLTLDRPDALNAITLDMVAELNDYFGRLYNDGSVRVVVMRGAG
CCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
KAYCAGLDIKERQGEDQEFPFGGGFGFQGFLADVYVKMRRCPQVILSLVHGPACGGGFAF
CEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ALASDIRLAGESARMNAAFIKIGLSSCDMGVSYFLPRLVGQSIASELMLTGRFIHAERAL
HHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGLVSEVVPDDQLESAAQGWIDDLLAASPKGLRMTKEGLQIATDAGSLEAAMAIENRNQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEECCCCHHHHHHHHCCHH
LMTSASPNFHEGMRAFLEKRKPDYTPD
HHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]