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The map label for this gene is thlA [H]

Identifier: 85374168

GI number: 85374168

Start: 1388234

End: 1389430

Strand: Direct

Name: thlA [H]

Synonym: ELI_06705

Alternate gene names: 85374168

Gene position: 1388234-1389430 (Clockwise)

Preceding gene: 85374142

Following gene: 85374169

Centisome position: 45.48

GC content: 66.42

Gene sequence:

>1197_bases
ATGACCCAGTTCGCCGCCTCCGACCCCGTCGTAATCCTCTCCTACGCCCGCACCCCGATGGGCGGCATGCAGGGCGCGCT
CGCCGATGTCTCGGCCACCGATCTTGGGGCGACGGCGGTGAAGGCGGCGGTCGAGCGCTCGGGCGTGCCGGTCGACAAGT
TCGACCGCACCTACATGGGTTGCGTCCTGCCGGCTGGTCTCGGCCAGGCCCCTGCCCGCCAGGCTTCGATCAAGGCGGGC
CTGCCGAAATCGGTCCAGGCGACTACCGTCAACAAGGTTTGCGGCAGCGGCATGCAGACGGTCATCATGGGCGCAGAAGC
GCTCGCCAGCGGCACGGTGGACTATGTCGTCTCCGGCGGCATGGAGAGCATGACCAACGCGCCCTATCTCTTGAAAAAGC
ACCGCAGCGGCGCGCGGCTGGGCCACGATACGACCTACGACCACATGTTCCTCGACGGGCTGGAAGATGCCTACGAGGAA
GGCCGGGCGATGGGCACCTTTGCGCAGGATACCGCAAACGAATACCAGCTCACCCGCGAGCAGATGGACGACTATTCGAT
CGAATCGCTGCGCCGCGCCAATGCCGCGATCGAGAGCGGCGCTTTCGCCGACGAGGTCGTGCCGGTCAGCTTCTCGACCC
GCCGCGGCGACGTGACGGTCGAGCATGACGAGCAGCCCGGCAAAGGCAAGCCGGACAAGATCCCGACCCTGCGGCCCGCA
TTCGCCAAGGATGGCACGATTACCGCGGCGACCTCCTCCTCGATCTCCGACGGCGCGGCGGCCGTCGTCTTGACGCGCGA
GAGCCTCGCGAGAGAGAATGGCCAGACACCCGTCGCGAAGATCGTCGCCATGGCAGCGCACGCGCAGGAGCCTTCCGAGT
TCACGGTGGCTCCGATCGGCGCGATCGAGAAGGTGCTCGAGAAAGCCGGCTGGTCCGCGGACGAGGTCGATCTGTGGGAA
GTCAACGAAGCCTTCGCCTGCGTCGCCATGTTCGCCATGCGCGACATCGGCATCCCGCACGACAAGATCAACATCAACGG
CGGCGGCACCGCACTCGGCCACCCGATCGGCGCGAGCGGCACGCGCATAATCGTGACGCTTCTCAACGCCTTGAAAAACC
AGGGCAAGACGAAGGGCGTTGCCAGCCTCTGCATCGGCGGCGGCGAAGCGACTGCGGTGGCGGTCGAACTCGTCTGA

Upstream 100 bases:

>100_bases
TCCTGCCTATCGCGCCTGCAAACGTATCCTGTTTGAATTTGCCGCAGTGCAGCACTAGGTGATCAACCAAGCAATCGATT
CACCAGAAAGGTCCCTCCCA

Downstream 100 bases:

>100_bases
GCCAGCCGCGCGCCGATTCGGCGTTTCCTTTCAGCGGTGTTACACTGTCGGCCTCTTTGAGAGGGAGGTTCGATATGAAG
AAGCTCGTTGTATGTTTGAG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTQFAASDPVVILSYARTPMGGMQGALADVSATDLGATAVKAAVERSGVPVDKFDRTYMGCVLPAGLGQAPARQASIKAG
LPKSVQATTVNKVCGSGMQTVIMGAEALASGTVDYVVSGGMESMTNAPYLLKKHRSGARLGHDTTYDHMFLDGLEDAYEE
GRAMGTFAQDTANEYQLTREQMDDYSIESLRRANAAIESGAFADEVVPVSFSTRRGDVTVEHDEQPGKGKPDKIPTLRPA
FAKDGTITAATSSSISDGAAAVVLTRESLARENGQTPVAKIVAMAAHAQEPSEFTVAPIGAIEKVLEKAGWSADEVDLWE
VNEAFACVAMFAMRDIGIPHDKININGGGTALGHPIGASGTRIIVTLLNALKNQGKTKGVASLCIGGGEATAVAVELV

Sequences:

>Translated_398_residues
MTQFAASDPVVILSYARTPMGGMQGALADVSATDLGATAVKAAVERSGVPVDKFDRTYMGCVLPAGLGQAPARQASIKAG
LPKSVQATTVNKVCGSGMQTVIMGAEALASGTVDYVVSGGMESMTNAPYLLKKHRSGARLGHDTTYDHMFLDGLEDAYEE
GRAMGTFAQDTANEYQLTREQMDDYSIESLRRANAAIESGAFADEVVPVSFSTRRGDVTVEHDEQPGKGKPDKIPTLRPA
FAKDGTITAATSSSISDGAAAVVLTRESLARENGQTPVAKIVAMAAHAQEPSEFTVAPIGAIEKVLEKAGWSADEVDLWE
VNEAFACVAMFAMRDIGIPHDKININGGGTALGHPIGASGTRIIVTLLNALKNQGKTKGVASLCIGGGEATAVAVELV
>Mature_397_residues
TQFAASDPVVILSYARTPMGGMQGALADVSATDLGATAVKAAVERSGVPVDKFDRTYMGCVLPAGLGQAPARQASIKAGL
PKSVQATTVNKVCGSGMQTVIMGAEALASGTVDYVVSGGMESMTNAPYLLKKHRSGARLGHDTTYDHMFLDGLEDAYEEG
RAMGTFAQDTANEYQLTREQMDDYSIESLRRANAAIESGAFADEVVPVSFSTRRGDVTVEHDEQPGKGKPDKIPTLRPAF
AKDGTITAATSSSISDGAAAVVLTRESLARENGQTPVAKIVAMAAHAQEPSEFTVAPIGAIEKVLEKAGWSADEVDLWEV
NEAFACVAMFAMRDIGIPHDKININGGGTALGHPIGASGTRIIVTLLNALKNQGKTKGVASLCIGGGEATAVAVELV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=44.0506329113924, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=43.4447300771208, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=36.2244897959184, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=32.7543424317618, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=30.6413301662708, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=26.8354430379747, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=47.680412371134, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=43.0412371134021, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787663, Length=394, Percent_Identity=36.0406091370558, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=35.8910891089109, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=29.5399515738499, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=43.1876606683805, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=40.2597402597403, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI133906874, Length=394, Percent_Identity=36.8020304568528, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=31.8877551020408, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=28.978622327791, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=43, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=31.2977099236641, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=44.2159383033419, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=43.4447300771208, Blast_Score=313, Evalue=9e-86,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=32.258064516129, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=28.3018867924528, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41463; Mature: 41332

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFAASDPVVILSYARTPMGGMQGALADVSATDLGATAVKAAVERSGVPVDKFDRTYMG
CCCCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHCCCCHHHHCCHHHH
CVLPAGLGQAPARQASIKAGLPKSVQATTVNKVCGSGMQTVIMGAEALASGTVDYVVSGG
HCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCC
MESMTNAPYLLKKHRSGARLGHDTTYDHMFLDGLEDAYEEGRAMGTFAQDTANEYQLTRE
HHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHH
QMDDYSIESLRRANAAIESGAFADEVVPVSFSTRRGDVTVEHDEQPGKGKPDKIPTLRPA
HHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCC
FAKDGTITAATSSSISDGAAAVVLTRESLARENGQTPVAKIVAMAAHAQEPSEFTVAPIG
CCCCCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECHH
AIEKVLEKAGWSADEVDLWEVNEAFACVAMFAMRDIGIPHDKININGGGTALGHPIGASG
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC
TRIIVTLLNALKNQGKTKGVASLCIGGGEATAVAVELV
HHHHHHHHHHHHCCCCCCCHHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TQFAASDPVVILSYARTPMGGMQGALADVSATDLGATAVKAAVERSGVPVDKFDRTYMG
CCCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHCCCCHHHHCCHHHH
CVLPAGLGQAPARQASIKAGLPKSVQATTVNKVCGSGMQTVIMGAEALASGTVDYVVSGG
HCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCC
MESMTNAPYLLKKHRSGARLGHDTTYDHMFLDGLEDAYEEGRAMGTFAQDTANEYQLTRE
HHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHH
QMDDYSIESLRRANAAIESGAFADEVVPVSFSTRRGDVTVEHDEQPGKGKPDKIPTLRPA
HHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCC
FAKDGTITAATSSSISDGAAAVVLTRESLARENGQTPVAKIVAMAAHAQEPSEFTVAPIG
CCCCCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECHH
AIEKVLEKAGWSADEVDLWEVNEAFACVAMFAMRDIGIPHDKININGGGTALGHPIGASG
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC
TRIIVTLLNALKNQGKTKGVASLCIGGGEATAVAVELV
HHHHHHHHHHHHCCCCCCCHHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]