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The map label for this gene is mscS [H]

Identifier: 85374112

GI number: 85374112

Start: 1334882

End: 1335775

Strand: Direct

Name: mscS [H]

Synonym: ELI_06425

Alternate gene names: 85374112

Gene position: 1334882-1335775 (Clockwise)

Preceding gene: 85374111

Following gene: 85374113

Centisome position: 43.73

GC content: 59.96

Gene sequence:

>894_bases
GTGGCGCCGGACGAGAGTTTCGACCGCACCGTAAACACGATCGCAACCTTGCAGGATCAGCTGGTCGGCATGGGCGAAGG
TTTGATCCGCGCCCTGCCCAACCTGGCCATTGCGCTGTTCATTCTGTTCATCACCTGGATCATCGCCAAATTCGCCGCGA
AGATCGCCGATGGACTGACCGGCAAGACCGAAATCCGGCCCAGCCTCAAGAACCTTATCGACACCGTCGTAAAACTGGCG
ATCTGGATCGTCGGCCTGATGGTGGCGCTGATCGTGGTCATGCCCGGCATGACGCCTGCGAGCCTGATCGCCGGCCTCGG
TATCGGCGCGGTCGCGATCGGTTTCGCGTTCCAGGACATTTTCGAGAATTTCCTCGCCGGCGTGCTCATCATGCTGCGCG
AGAAGATGCGGATTGGCGATGTGATCGAGTGCGAAGGCATCATCGGCAAGGTCGAGCACATCACCTTGCGCGAAACCTAT
GTCCGCAAGTTGTCGGGTGAAGTGACGCTGGTGCCGAACTCGATGCTGTTCAAGAACCCGGTCGAGATCCTTACCGACGA
GACCGAGCGCCGGCACGAAGTCGTGGCCGGGGTCTCCTACGACACAGATCTCGATCATGCGGCCGAAGTTATTCGCAAAG
CTGTCGAAGGCGTCGACAATGTCCTCACCGACAAGGGTATCGACGTGTTCGCGAGCGAGTTCGCCGACAGCTCTGTGAAC
TTCAACGTGCGATGGTGGGCAGGCTCCAAACCGCGCGACATGCACGAAAGCCGCGACCGCGTGGTCCGCGCCATCAAGCG
GGCGCTGGACGATGCTGGGATCGAGATTCCGTTCCCTTACATCACCCATACCTTCAAGGAACGGGTTCCGCTTGGGAGCG
CCAGCGAAAGCTGA

Upstream 100 bases:

>100_bases
GAGCACTCGCGCGTCGCATGTTGCAAATTCCGGAACCCCTGCGAATATCGCCCGTTTTGCGGGTAAGTTCAGAGGGGTTT
TCATGACACAACAGACACCG

Downstream 100 bases:

>100_bases
TAGCCGCCTCGGGTGGCAGACGACGATAGCGACCCCGGCGCCCGCGCCGGTCTGAGCAGGGCCGACCAATGGTGGTTGGC
CCTGCTTACGGGCGTGACGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAPDESFDRTVNTIATLQDQLVGMGEGLIRALPNLAIALFILFITWIIAKFAAKIADGLTGKTEIRPSLKNLIDTVVKLA
IWIVGLMVALIVVMPGMTPASLIAGLGIGAVAIGFAFQDIFENFLAGVLIMLREKMRIGDVIECEGIIGKVEHITLRETY
VRKLSGEVTLVPNSMLFKNPVEILTDETERRHEVVAGVSYDTDLDHAAEVIRKAVEGVDNVLTDKGIDVFASEFADSSVN
FNVRWWAGSKPRDMHESRDRVVRAIKRALDDAGIEIPFPYITHTFKERVPLGSASES

Sequences:

>Translated_297_residues
MAPDESFDRTVNTIATLQDQLVGMGEGLIRALPNLAIALFILFITWIIAKFAAKIADGLTGKTEIRPSLKNLIDTVVKLA
IWIVGLMVALIVVMPGMTPASLIAGLGIGAVAIGFAFQDIFENFLAGVLIMLREKMRIGDVIECEGIIGKVEHITLRETY
VRKLSGEVTLVPNSMLFKNPVEILTDETERRHEVVAGVSYDTDLDHAAEVIRKAVEGVDNVLTDKGIDVFASEFADSSVN
FNVRWWAGSKPRDMHESRDRVVRAIKRALDDAGIEIPFPYITHTFKERVPLGSASES
>Mature_296_residues
APDESFDRTVNTIATLQDQLVGMGEGLIRALPNLAIALFILFITWIIAKFAAKIADGLTGKTEIRPSLKNLIDTVVKLAI
WIVGLMVALIVVMPGMTPASLIAGLGIGAVAIGFAFQDIFENFLAGVLIMLREKMRIGDVIECEGIIGKVEHITLRETYV
RKLSGEVTLVPNSMLFKNPVEILTDETERRHEVVAGVSYDTDLDHAAEVIRKAVEGVDNVLTDKGIDVFASEFADSSVNF
NVRWWAGSKPRDMHESRDRVVRAIKRALDDAGIEIPFPYITHTFKERVPLGSASES

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=254, Percent_Identity=30.3149606299213, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786670, Length=179, Percent_Identity=28.4916201117318, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367355, Length=247, Percent_Identity=22.6720647773279, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787591, Length=176, Percent_Identity=28.4090909090909, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32618; Mature: 32487

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPDESFDRTVNTIATLQDQLVGMGEGLIRALPNLAIALFILFITWIIAKFAAKIADGLT
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKTEIRPSLKNLIDTVVKLAIWIVGLMVALIVVMPGMTPASLIAGLGIGAVAIGFAFQDI
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
FENFLAGVLIMLREKMRIGDVIECEGIIGKVEHITLRETYVRKLSGEVTLVPNSMLFKNP
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCC
VEILTDETERRHEVVAGVSYDTDLDHAAEVIRKAVEGVDNVLTDKGIDVFASEFADSSVN
HHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
FNVRWWAGSKPRDMHESRDRVVRAIKRALDDAGIEIPFPYITHTFKERVPLGSASES
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
APDESFDRTVNTIATLQDQLVGMGEGLIRALPNLAIALFILFITWIIAKFAAKIADGLT
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKTEIRPSLKNLIDTVVKLAIWIVGLMVALIVVMPGMTPASLIAGLGIGAVAIGFAFQDI
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
FENFLAGVLIMLREKMRIGDVIECEGIIGKVEHITLRETYVRKLSGEVTLVPNSMLFKNP
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCC
VEILTDETERRHEVVAGVSYDTDLDHAAEVIRKAVEGVDNVLTDKGIDVFASEFADSSVN
HHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
FNVRWWAGSKPRDMHESRDRVVRAIKRALDDAGIEIPFPYITHTFKERVPLGSASES
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]