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The map label for this gene is trmFO

Identifier: 85374098

GI number: 85374098

Start: 1321498

End: 1322865

Strand: Direct

Name: trmFO

Synonym: ELI_06355

Alternate gene names: 85374098

Gene position: 1321498-1322865 (Clockwise)

Preceding gene: 85374096

Following gene: 85374101

Centisome position: 43.29

GC content: 61.48

Gene sequence:

>1368_bases
ATGTCTCATGATGTGCACATAATCGGCGGCGGCCTCGCCGGAAGCGAAGCGGCATGGCAGCTCGCGCGACGCGGCGTGAA
GGTGCGCCTGTCGGAAATGCGGGGGTCAGGTGAAATGACACACGCGCACCAGACCGACGGCCTGGCCGAACTGGTGTGTT
CGAACAGTTTTCGCTCCGACGACGATACCAAAAACGCCGTCGGTCTTCTGCACTATGAGATGCGCGCGCTCGACAGCTTG
GTAATGCGCGCAGGCGAAGTTGCGCAGGTGCCCGCGGGCAGTGCCATGGCGGTGGATCGCGATGTCTTCTCCGCCGAGGT
CCAGAAGACGTTGGAGGCGCATCCCAATATCGAAGTGGTGCGTGAGCGAGTTGATGCCTTGCCGGAAGCAGGGCTCACGA
TCGTTTCGACAGGGCCCCTCACCGCGCAGTCGCTGGCCGAGAGCATCGTGAAAGCTACCGGGCAGGAACGGCTGGCCTTT
TTCGATGCCATCGCCCCGATCGTCCATCGCGACAGCATCGACATGTCGAAATGCTGGATCCAATCGCGCTGGAACAAGCG
CACCGAGGCCTCCAACGAAGACGGCGACTACATCAATTGCCCGATGACGAAGGAGCAATATCTCGCCTTCCACCAGGGCC
TGATCGACGGCGAGAAGACCGAGTTTCGCGAGTGGGAAAAGGACACGCCCTATTTCGATGGCTGCATGCCGATCGAAATC
ATGGCGGCGCGCGGGGTGGAAACGCTGCGCTATGGCCCGATGAAAGGCGTCGGGCTCGATAATCCTTACGATACGAGCGA
GGAACATCCGCAGGGACGGTGGCCGTATGCCGTCGTGCAATTGCGGCAGGACAACAAGCTCGGCACGCTGTGGAACATGG
TCGGGTTCCAGACCAAGCTGAAATATGGCGCGCAGGTCGAGCTGTTTCGCACCATTCCGGGGCTCGAGAATGCCGAATTC
GCGCGGCTTGGTGGGCTGCATCGCAACACCTTCCTTAACTCGCCAATGGTGCTCGACCGGCAGCTTAGGCTGAAGGGGGC
GGAGCACATCCGGTTTGCGGGACAGGTTACCGGCTGCGAGGGTTATGTCGAAAGCGCGGCCGTCGGTCTCATGGCCGGGA
TGATGACCGCCAAGGAACTTGCCGGCGACGTCTGGCAGGGTCCGCCGCGCACGACCGCGCTCGGCGCGCTGCTCTCGCAC
ATCACCGGCGATGCCGAAGCGGAAACCTATCAGCCGATGAACATCAATTTTGGACTGTTCCCGCCGCTGCACGAGGTCAA
GAAGAAGCAGCGCAAGGAAGCCTATACGACCCGCGCGAAAAGCGATCTCGGCGAGTGGATTGCCCACGAGGTACGAGTCT
CTGCGTAG

Upstream 100 bases:

>100_bases
CAAGGGCGATGTTCGATCAAGGCGCGTTTGGGCATGGGCGAGGGTCCTCGCGCCAGCGCTAGGGCTTGATGGGGCTGCGC
TGCAAGCCTAACTGCGCGCC

Downstream 100 bases:

>100_bases
GCGGGAACCTCAGGCTATCAGCCCCGCGCCAATCTCCTGAGACCCCCGCCTTCGCGAGGGATCCCCTAACAGCGGCAATT
GTTTTGGCGCGCCCGTCGTT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHDVHIIGGGLAGSEAAWQLARRGVKVRLSEMRGSGEMTHAHQTDGLAELVCSNSFRSDDDTKNAVGLLHYEMRALDSL
VMRAGEVAQVPAGSAMAVDRDVFSAEVQKTLEAHPNIEVVRERVDALPEAGLTIVSTGPLTAQSLAESIVKATGQERLAF
FDAIAPIVHRDSIDMSKCWIQSRWNKRTEASNEDGDYINCPMTKEQYLAFHQGLIDGEKTEFREWEKDTPYFDGCMPIEI
MAARGVETLRYGPMKGVGLDNPYDTSEEHPQGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKYGAQVELFRTIPGLENAEF
ARLGGLHRNTFLNSPMVLDRQLRLKGAEHIRFAGQVTGCEGYVESAAVGLMAGMMTAKELAGDVWQGPPRTTALGALLSH
ITGDAEAETYQPMNINFGLFPPLHEVKKKQRKEAYTTRAKSDLGEWIAHEVRVSA

Sequences:

>Translated_455_residues
MSHDVHIIGGGLAGSEAAWQLARRGVKVRLSEMRGSGEMTHAHQTDGLAELVCSNSFRSDDDTKNAVGLLHYEMRALDSL
VMRAGEVAQVPAGSAMAVDRDVFSAEVQKTLEAHPNIEVVRERVDALPEAGLTIVSTGPLTAQSLAESIVKATGQERLAF
FDAIAPIVHRDSIDMSKCWIQSRWNKRTEASNEDGDYINCPMTKEQYLAFHQGLIDGEKTEFREWEKDTPYFDGCMPIEI
MAARGVETLRYGPMKGVGLDNPYDTSEEHPQGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKYGAQVELFRTIPGLENAEF
ARLGGLHRNTFLNSPMVLDRQLRLKGAEHIRFAGQVTGCEGYVESAAVGLMAGMMTAKELAGDVWQGPPRTTALGALLSH
ITGDAEAETYQPMNINFGLFPPLHEVKKKQRKEAYTTRAKSDLGEWIAHEVRVSA
>Mature_454_residues
SHDVHIIGGGLAGSEAAWQLARRGVKVRLSEMRGSGEMTHAHQTDGLAELVCSNSFRSDDDTKNAVGLLHYEMRALDSLV
MRAGEVAQVPAGSAMAVDRDVFSAEVQKTLEAHPNIEVVRERVDALPEAGLTIVSTGPLTAQSLAESIVKATGQERLAFF
DAIAPIVHRDSIDMSKCWIQSRWNKRTEASNEDGDYINCPMTKEQYLAFHQGLIDGEKTEFREWEKDTPYFDGCMPIEIM
AARGVETLRYGPMKGVGLDNPYDTSEEHPQGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKYGAQVELFRTIPGLENAEFA
RLGGLHRNTFLNSPMVLDRQLRLKGAEHIRFAGQVTGCEGYVESAAVGLMAGMMTAKELAGDVWQGPPRTTALGALLSHI
TGDAEAETYQPMNINFGLFPPLHEVKKKQRKEAYTTRAKSDLGEWIAHEVRVSA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_ERYLH (Q2NAC8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458160.1
- STRING:   Q2NAC8
- GeneID:   3871190
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06355
- NMPDR:   fig|314225.3.peg.1785
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   ELIT314225:ELI_06355-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50303; Mature: 50171

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDVHIIGGGLAGSEAAWQLARRGVKVRLSEMRGSGEMTHAHQTDGLAELVCSNSFRSD
CCCCEEEEECCCCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCHHCCCHHHHHHCCCCCCC
DDTKNAVGLLHYEMRALDSLVMRAGEVAQVPAGSAMAVDRDVFSAEVQKTLEAHPNIEVV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHH
RERVDALPEAGLTIVSTGPLTAQSLAESIVKATGQERLAFFDAIAPIVHRDSIDMSKCWI
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHH
QSRWNKRTEASNEDGDYINCPMTKEQYLAFHQGLIDGEKTEFREWEKDTPYFDGCMPIEI
HHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHH
MAARGVETLRYGPMKGVGLDNPYDTSEEHPQGRWPYAVVQLRQDNKLGTLWNMVGFQTKL
HHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
KYGAQVELFRTIPGLENAEFARLGGLHRNTFLNSPMVLDRQLRLKGAEHIRFAGQVTGCE
CCCCEEEEEECCCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCCCEEEECEECCCH
GYVESAAVGLMAGMMTAKELAGDVWQGPPRTTALGALLSHITGDAEAETYQPMNINFGLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PPLHEVKKKQRKEAYTTRAKSDLGEWIAHEVRVSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHDVHIIGGGLAGSEAAWQLARRGVKVRLSEMRGSGEMTHAHQTDGLAELVCSNSFRSD
CCCEEEEECCCCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCHHCCCHHHHHHCCCCCCC
DDTKNAVGLLHYEMRALDSLVMRAGEVAQVPAGSAMAVDRDVFSAEVQKTLEAHPNIEVV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHH
RERVDALPEAGLTIVSTGPLTAQSLAESIVKATGQERLAFFDAIAPIVHRDSIDMSKCWI
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHH
QSRWNKRTEASNEDGDYINCPMTKEQYLAFHQGLIDGEKTEFREWEKDTPYFDGCMPIEI
HHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHH
MAARGVETLRYGPMKGVGLDNPYDTSEEHPQGRWPYAVVQLRQDNKLGTLWNMVGFQTKL
HHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
KYGAQVELFRTIPGLENAEFARLGGLHRNTFLNSPMVLDRQLRLKGAEHIRFAGQVTGCE
CCCCEEEEEECCCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCCCEEEECEECCCH
GYVESAAVGLMAGMMTAKELAGDVWQGPPRTTALGALLSHITGDAEAETYQPMNINFGLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PPLHEVKKKQRKEAYTTRAKSDLGEWIAHEVRVSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA