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The map label for this gene is icfA [H]

Identifier: 85374092

GI number: 85374092

Start: 1314028

End: 1314669

Strand: Direct

Name: icfA [H]

Synonym: ELI_06325

Alternate gene names: 85374092

Gene position: 1314028-1314669 (Clockwise)

Preceding gene: 85374091

Following gene: 85374093

Centisome position: 43.05

GC content: 62.77

Gene sequence:

>642_bases
ATGCCCGAATATGCCGAGCTGCTAAGAGGCTACCGTCGCTTTCGCGAAGGCACCTACCCGCGCCAGCGCGCGCGGTTCGA
GGAAACGGCCATGGAAGGCCAGCACCCTAAGCTGATGATCATCGGCTGCAGCGACAGCCGCGTCGATCCGGCGCAAATCT
TCGATGTCGATCCGGGCGAGATCTTTGTGGTCCGCAATGTCGCCGCGCTCGTGCCGCCATTCGAGACGACGCCGGGTCTC
CATGGGGTATCGGCCGCGGTCGAGTTCGCCGTGCAGATGCTGGAGGTGAAGCAGATCCTGGTCATGGGACACGGCCGCTG
CGGCGGTTGCAAGGCGGCACTGACGGAAAATCTTGCCGCCGCCTCTCCCGGCGAGGGCGGATTCGTCGCCAACTGGATCA
AACTTCTCGACGAAGCCCGGAAACCGGTGGCAGAGAAACACGGGACATCGGGTCGCGAGGCGGAATTGGCGATGGAGTTC
GCCGCCGTCCGTCAAAGCCTGGCCAATCTGCGCACGTTTCCGTGGGTCGCCGAAAAGGAGACGGCTGGCGAAATCAAGCT
GCGCGGGGCGCACTTCTCGATCAAGGAAGGGGTGCTCTATTCGCTCGACGAAGACACGGGCGAATTTCTTCCCGAAGACT
AA

Upstream 100 bases:

>100_bases
TGCTTACGAAATATGGATTCATCCTGAGCATGTGGGACGTACAGGTTATCCGGGGGGAGCGCTTCTAGGTATCGGCGCCT
CTCCATTGAGGGAGGGACAG

Downstream 100 bases:

>100_bases
TTGCTTGCCCTCTATCGACCCGAGGACCATCTAGCGCGCCATGCCCGATACCGAACTAAAAAACATCGCCCTGCTGATCG
ATGCCGACAATACGACGCCG

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPEYAELLRGYRRFREGTYPRQRARFEETAMEGQHPKLMIIGCSDSRVDPAQIFDVDPGEIFVVRNVAALVPPFETTPGL
HGVSAAVEFAVQMLEVKQILVMGHGRCGGCKAALTENLAAASPGEGGFVANWIKLLDEARKPVAEKHGTSGREAELAMEF
AAVRQSLANLRTFPWVAEKETAGEIKLRGAHFSIKEGVLYSLDEDTGEFLPED

Sequences:

>Translated_213_residues
MPEYAELLRGYRRFREGTYPRQRARFEETAMEGQHPKLMIIGCSDSRVDPAQIFDVDPGEIFVVRNVAALVPPFETTPGL
HGVSAAVEFAVQMLEVKQILVMGHGRCGGCKAALTENLAAASPGEGGFVANWIKLLDEARKPVAEKHGTSGREAELAMEF
AAVRQSLANLRTFPWVAEKETAGEIKLRGAHFSIKEGVLYSLDEDTGEFLPED
>Mature_212_residues
PEYAELLRGYRRFREGTYPRQRARFEETAMEGQHPKLMIIGCSDSRVDPAQIFDVDPGEIFVVRNVAALVPPFETTPGLH
GVSAAVEFAVQMLEVKQILVMGHGRCGGCKAALTENLAAASPGEGGFVANWIKLLDEARKPVAEKHGTSGREAELAMEFA
AVRQSLANLRTFPWVAEKETAGEIKLRGAHFSIKEGVLYSLDEDTGEFLPED

Specific function: Reversible hydration of carbon dioxide. Essential to photosynthetic carbon dioxide fixation [H]

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=206, Percent_Identity=33.0097087378641, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786318, Length=185, Percent_Identity=30.2702702702703, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324292, Length=187, Percent_Identity=26.2032085561497, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23404; Mature: 23273

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYAELLRGYRRFREGTYPRQRARFEETAMEGQHPKLMIIGCSDSRVDPAQIFDVDPGE
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHEEECCCCC
IFVVRNVAALVPPFETTPGLHGVSAAVEFAVQMLEVKQILVMGHGRCGGCKAALTENLAA
EEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ASPGEGGFVANWIKLLDEARKPVAEKHGTSGREAELAMEFAAVRQSLANLRTFPWVAEKE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TAGEIKLRGAHFSIKEGVLYSLDEDTGEFLPED
CCCCEEEECCCEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PEYAELLRGYRRFREGTYPRQRARFEETAMEGQHPKLMIIGCSDSRVDPAQIFDVDPGE
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHEEECCCCC
IFVVRNVAALVPPFETTPGLHGVSAAVEFAVQMLEVKQILVMGHGRCGGCKAALTENLAA
EEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ASPGEGGFVANWIKLLDEARKPVAEKHGTSGREAELAMEFAAVRQSLANLRTFPWVAEKE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TAGEIKLRGAHFSIKEGVLYSLDEDTGEFLPED
CCCCEEEECCCEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1584776 [H]