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The map label for this gene is proS

Identifier: 85374048

GI number: 85374048

Start: 1263618

End: 1265162

Strand: Direct

Name: proS

Synonym: ELI_06105

Alternate gene names: 85374048

Gene position: 1263618-1265162 (Clockwise)

Preceding gene: 85374047

Following gene: 85374049

Centisome position: 41.4

GC content: 61.1

Gene sequence:

>1545_bases
GTGTCGAACATCCGCCACGCCCTAACCGTAAAGCGAGAAGACGATTTCGCTGCCTGGTATCAGGCAGTCATCGCCGAGGC
CGACCTTGCCGAAGAATCCGGCGTCCGCGGCTGCATGGTCATCAAGCCATGGGGCTACGGCATATGGGAACGCATCCAGC
GCCTGCTCGACGACCGTATCAAGGCCACGGGCCATGAAAACGTTTACTTCCCGATCTTCATCCCGCTTTCCAATTTCGAG
CGGGAGGCCGAGCATGTCGAGGGCTTTGCCAAGGAAATGGCAGTTGTCACTCACCACCGGCTGATCGGTGATGGCGAGGG
CGGGTTGATCCCCGATCCCGAAGCCAAGCTGGAAGAGCCGCTCGTGGTGCGCCCCACATCGGAAACGATCTTCGGCGATG
CCATGGCGCGCTGGATCCAAAGCTGGCGCGACCTGCCGTTGCTCACAAACCAATGGGCGAACGTCGTGCGCTGGGAAATG
CGCACCCGCATGTTTCTGCGCACCAGCGAGTTCCTCTGGCAGGAAGGGCATACCGCGCACGCCACCGCAGAGGAGGCCAA
GGAACACACCCTGACGATGCTAGAGGTCTATCGTGCGCATGCCGAGGAAGATCTGGCACTTCCGGTTATCGCCGGCGAGA
AACCCGAGAACGAGCGCTTCCCGGGAGCGGTCGAGACATGGTCGATCGAAGCCATGATGCAGGATGGCAAGGCGCTGCAG
GCCGGGACCAGCCACTATCTCGGGACCAACTTTTCCGAAGCGGCAAACATCAAATTCCAGGACCGAGAAGGCGGCGAAAA
ATATTGCCATACGACAAGCTGGGGCGTGTCGACCCGCATGATCGGCGGCGTCATCATGACCCATGGCGACGACGACGGGC
TCAAGGTTCCGCCCAGGATCGCTCCGTACCAGGTCGTGATCCTGCCGATGCTCCGGGACAAGCCCGAAGACGACGCGCTG
CTCGCCTATTGCGAAGACCTGCGCGGCAAACTCATCACTGAATCCGCGATGGGCGAACCCGTGCGCGTGCTGTTGGACAA
GAAGCCGGGCAAGGCCGCTGCCAAGCGCTGGAACTGGGTGCGCAAGGGCGCGCCGATCGTAATCGAGGTCGGCGGCCGCG
ATATGGAGAATGGCGTTGTCAGCCTGCTGCGCCGCGACGAACTGTGGAACGAGGAAACCGGCAAGCCGGCGTTCCAGGCC
CCCACACCGGCGCAATTGACGGATGAAATCGCTTCGATGCTGGATTCGATCCAGGCGGGCATGTTCGGCACGGCACAAAC
GTGGCGAAACGCCAATGTCATGAGCGGGTGCACATCTTTCGAGCAATTGCGCGACCATTATTCCGACGGCAAAAAACACG
TCGGCTGGGTCGAGGTCCAGTGGTCGAAGCCTACCGGTCAGGAGCTCGAGAAAGTCGTCGAGAAGCTCAAGGACCTGAAG
CTGACGATCCGCAACGTGCCGATGGATACTCCCGCGCCGACGGGCTCCTGCATCTTCACCGGCAAACCGGCGGTCGAGTG
GATATACGTCGCAAAGGCATATTGA

Upstream 100 bases:

>100_bases
AAGCAAATGGCTGAGATGCAGAAGAAGCTGGACGAGCTCGGCAAGTAAGGCCAAGCGTCCGCGCAATCTACCACCCATCA
TTCACAGGACAGGCGCACCC

Downstream 100 bases:

>100_bases
CCGCGGGCGTGGTGCGCGCCCATGATGCCGCCATGCGCAAATTCCTGATTGTTACCACTTTCCTCGCCGCCCCGCTCGCG
GCGCAGGACCATCCTGCCGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSNIRHALTVKREDDFAAWYQAVIAEADLAEESGVRGCMVIKPWGYGIWERIQRLLDDRIKATGHENVYFPIFIPLSNFE
REAEHVEGFAKEMAVVTHHRLIGDGEGGLIPDPEAKLEEPLVVRPTSETIFGDAMARWIQSWRDLPLLTNQWANVVRWEM
RTRMFLRTSEFLWQEGHTAHATAEEAKEHTLTMLEVYRAHAEEDLALPVIAGEKPENERFPGAVETWSIEAMMQDGKALQ
AGTSHYLGTNFSEAANIKFQDREGGEKYCHTTSWGVSTRMIGGVIMTHGDDDGLKVPPRIAPYQVVILPMLRDKPEDDAL
LAYCEDLRGKLITESAMGEPVRVLLDKKPGKAAAKRWNWVRKGAPIVIEVGGRDMENGVVSLLRRDELWNEETGKPAFQA
PTPAQLTDEIASMLDSIQAGMFGTAQTWRNANVMSGCTSFEQLRDHYSDGKKHVGWVEVQWSKPTGQELEKVVEKLKDLK
LTIRNVPMDTPAPTGSCIFTGKPAVEWIYVAKAY

Sequences:

>Translated_514_residues
MSNIRHALTVKREDDFAAWYQAVIAEADLAEESGVRGCMVIKPWGYGIWERIQRLLDDRIKATGHENVYFPIFIPLSNFE
REAEHVEGFAKEMAVVTHHRLIGDGEGGLIPDPEAKLEEPLVVRPTSETIFGDAMARWIQSWRDLPLLTNQWANVVRWEM
RTRMFLRTSEFLWQEGHTAHATAEEAKEHTLTMLEVYRAHAEEDLALPVIAGEKPENERFPGAVETWSIEAMMQDGKALQ
AGTSHYLGTNFSEAANIKFQDREGGEKYCHTTSWGVSTRMIGGVIMTHGDDDGLKVPPRIAPYQVVILPMLRDKPEDDAL
LAYCEDLRGKLITESAMGEPVRVLLDKKPGKAAAKRWNWVRKGAPIVIEVGGRDMENGVVSLLRRDELWNEETGKPAFQA
PTPAQLTDEIASMLDSIQAGMFGTAQTWRNANVMSGCTSFEQLRDHYSDGKKHVGWVEVQWSKPTGQELEKVVEKLKDLK
LTIRNVPMDTPAPTGSCIFTGKPAVEWIYVAKAY
>Mature_513_residues
SNIRHALTVKREDDFAAWYQAVIAEADLAEESGVRGCMVIKPWGYGIWERIQRLLDDRIKATGHENVYFPIFIPLSNFER
EAEHVEGFAKEMAVVTHHRLIGDGEGGLIPDPEAKLEEPLVVRPTSETIFGDAMARWIQSWRDLPLLTNQWANVVRWEMR
TRMFLRTSEFLWQEGHTAHATAEEAKEHTLTMLEVYRAHAEEDLALPVIAGEKPENERFPGAVETWSIEAMMQDGKALQA
GTSHYLGTNFSEAANIKFQDREGGEKYCHTTSWGVSTRMIGGVIMTHGDDDGLKVPPRIAPYQVVILPMLRDKPEDDALL
AYCEDLRGKLITESAMGEPVRVLLDKKPGKAAAKRWNWVRKGAPIVIEVGGRDMENGVVSLLRRDELWNEETGKPAFQAP
TPAQLTDEIASMLDSIQAGMFGTAQTWRNANVMSGCTSFEQLRDHYSDGKKHVGWVEVQWSKPTGQELEKVVEKLKDLKL
TIRNVPMDTPAPTGSCIFTGKPAVEWIYVAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=527, Percent_Identity=37.1916508538899, Blast_Score=323, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71991342, Length=444, Percent_Identity=36.2612612612613, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71991346, Length=371, Percent_Identity=39.0835579514825, Blast_Score=278, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6321808, Length=396, Percent_Identity=40.1515151515151, Blast_Score=292, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24649466, Length=520, Percent_Identity=35.1923076923077, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24649468, Length=446, Percent_Identity=37.6681614349776, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ERYLH (Q2NAH8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458110.1
- ProteinModelPortal:   Q2NAH8
- SMR:   Q2NAH8
- STRING:   Q2NAH8
- GeneID:   3870053
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06105
- NMPDR:   fig|314225.3.peg.1837
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   EGFAKEM
- PhylomeDB:   Q2NAH8
- ProtClustDB:   PRK08661
- BioCyc:   ELIT314225:ELI_06105-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 57840; Mature: 57709

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIRHALTVKREDDFAAWYQAVIAEADLAEESGVRGCMVIKPWGYGIWERIQRLLDDRI
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
KATGHENVYFPIFIPLSNFEREAEHVEGFAKEMAVVTHHRLIGDGEGGLIPDPEAKLEEP
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCCC
LVVRPTSETIFGDAMARWIQSWRDLPLLTNQWANVVRWEMRTRMFLRTSEFLWQEGHTAH
EEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAEEAKEHTLTMLEVYRAHAEEDLALPVIAGEKPENERFPGAVETWSIEAMMQDGKALQ
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHCCCHHH
AGTSHYLGTNFSEAANIKFQDREGGEKYCHTTSWGVSTRMIGGVIMTHGDDDGLKVPPRI
CCCCCCCCCCCCCCCCCEEECCCCCCHHEEECCCCCHHHHHCCEEEECCCCCCCCCCCCC
APYQVVILPMLRDKPEDDALLAYCEDLRGKLITESAMGEPVRVLLDKKPGKAAAKRWNWV
CCEEEEEEEHHCCCCCCHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCHHHHHHHHHH
RKGAPIVIEVGGRDMENGVVSLLRRDELWNEETGKPAFQAPTPAQLTDEIASMLDSIQAG
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
MFGTAQTWRNANVMSGCTSFEQLRDHYSDGKKHVGWVEVQWSKPTGQELEKVVEKLKDLK
CCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHE
LTIRNVPMDTPAPTGSCIFTGKPAVEWIYVAKAY
EHEECCCCCCCCCCCCEEECCCCCEEEEEEEECC
>Mature Secondary Structure 
SNIRHALTVKREDDFAAWYQAVIAEADLAEESGVRGCMVIKPWGYGIWERIQRLLDDRI
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
KATGHENVYFPIFIPLSNFEREAEHVEGFAKEMAVVTHHRLIGDGEGGLIPDPEAKLEEP
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCCC
LVVRPTSETIFGDAMARWIQSWRDLPLLTNQWANVVRWEMRTRMFLRTSEFLWQEGHTAH
EEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAEEAKEHTLTMLEVYRAHAEEDLALPVIAGEKPENERFPGAVETWSIEAMMQDGKALQ
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHCCCHHH
AGTSHYLGTNFSEAANIKFQDREGGEKYCHTTSWGVSTRMIGGVIMTHGDDDGLKVPPRI
CCCCCCCCCCCCCCCCCEEECCCCCCHHEEECCCCCHHHHHCCEEEECCCCCCCCCCCCC
APYQVVILPMLRDKPEDDALLAYCEDLRGKLITESAMGEPVRVLLDKKPGKAAAKRWNWV
CCEEEEEEEHHCCCCCCHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCHHHHHHHHHH
RKGAPIVIEVGGRDMENGVVSLLRRDELWNEETGKPAFQAPTPAQLTDEIASMLDSIQAG
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
MFGTAQTWRNANVMSGCTSFEQLRDHYSDGKKHVGWVEVQWSKPTGQELEKVVEKLKDLK
CCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHE
LTIRNVPMDTPAPTGSCIFTGKPAVEWIYVAKAY
EHEECCCCCCCCCCCCEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA