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The map label for this gene is radA [H]

Identifier: 85374042

GI number: 85374042

Start: 1257849

End: 1259216

Strand: Direct

Name: radA [H]

Synonym: ELI_06075

Alternate gene names: 85374042

Gene position: 1257849-1259216 (Clockwise)

Preceding gene: 85374040

Following gene: 85374043

Centisome position: 41.21

GC content: 64.99

Gene sequence:

>1368_bases
ATGGCGAAGACCAAACGCAAATATGTCTGCCAGGCCTGCGGCACCGTGGCGCATCGTTGGCAGGGGCAATGCGCGGAATG
TGCGGAGTGGAATTCGCTCGTCGAAGACGCGCCCGCGACTGTCTTTTCGCAAAAGCACGATCTTTCGAGCGGTGGGCGGG
CCATCACATTCGTCGACCTCAACCAGCCCGGCCACGAGCTGACGCGGCGTACCACCGGGCTGGCGGAGTTCGATCGCGCG
CTGGGCGGGGGGCTGGTCCCCGGCTCTGCCATCCTCATGGGCGGCGACCCGGGCATTGGCAAATCGACGCTGCTGTTGCA
GGCCGCCGCCAGCATTGCCAAGTCTGGACAGCAAGTGGTCTATGTCAGCGGCGAGGAAGCCGCCGGACAGGTCCGCATGC
GCGCCTCGCGGCTGGGCGTTGCCGATGCACCGCTCAAGCTCGCTTCTTCAACTTCGGTGCGCGATATCCTGACGACGCTC
GGCAGCATGGACGCTCCGGCGCTGCTGGTCATCGATTCGATCCAGACCATGCATTCGGACACGATCGAAGGAGCGCCGGG
CACGGTCAGCCAGGTTCGCGGATGCGCTTTCGAACTGATCCGCTATGCCAAGGAAAGCGGCACCGCGCTGGTCCTCGTCG
GCCATGTCACGAAGGACGGCAATATCGCTGGCCCGCGCGTGCTGGAGCACATGGTCGATGTCGTCATGAGCTTCGAAGGC
GAGCGCAGTCACCAGTACCGCATCCTACGCGCGCTCAAGAACCGTTTCGGCGCGGTCGACGAGATCGGTGTTTTCGCGAT
GGCGGGGCAGGGGCTCGAAGAGGTTGCCAACCCCTCGATGCTGTTCCTGTCCGGTCGCGACCAACCCTTGGCCGGCAGTG
CCGTCTTTCCTGCTCTCGAAGGGACACGCCCGGTGCTGGTGGAAGTGCAGGCGCTGATCGTCCGGCTCCAGTCCGGTGCG
ACCCCGCGTCGGGCGGTCGTGGGTTGGGACAATGGCCGTCTAGCCATGCTGCTGGCGGTGCTGGAATCGCGGTGCGGGCT
GAACTTCAGTTCGGCCGAGGTCTATCTGAACGTGGCGGGGGGCTATCGCTTGTCGGATCCGGCGGCCGACCTCGCCGTTG
CCGCGGCGCTTGTTTCGGCGCTCGCCGACAAGCCGCTGCCCGAGAATTCGGTCTGGTTCGGTGAAGTTTCACTGGCGGGC
GAGATACGCCCGGTGGCTCATGCCGGTCTGCGCTTGCGAGAAGCGGCCAAACTCGGCTTTTCGACCGGGTGCGGGCCGCA
AAATGGTGGGGAGAAGGTTGGCAAGATGCGATATGCGCCCGTCGATCGCCTGGGCAAGCTCGTTGACCGGGTCATGGGTA
CCTCCTAA

Upstream 100 bases:

>100_bases
CAAGGCAATGCGAAGTTCCTTCTGCCGCATCCGGGGATTGTGCCGATTGCTTCACCCATTGGCAATTGCGTTCTCTTAAT
GTTCCGACTACTTGTCCGCC

Downstream 100 bases:

>100_bases
TTCACCGGTCGTCATGACCATGTTCGATATCCTCGTCCTTCTGGTAGTCGGAGTTGCGGCGGTCGGCGGCTTCCTGCGCG
GGTTTGTGCAGGAAGTGCTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAKTKRKYVCQACGTVAHRWQGQCAECAEWNSLVEDAPATVFSQKHDLSSGGRAITFVDLNQPGHELTRRTTGLAEFDRA
LGGGLVPGSAILMGGDPGIGKSTLLLQAAASIAKSGQQVVYVSGEEAAGQVRMRASRLGVADAPLKLASSTSVRDILTTL
GSMDAPALLVIDSIQTMHSDTIEGAPGTVSQVRGCAFELIRYAKESGTALVLVGHVTKDGNIAGPRVLEHMVDVVMSFEG
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVANPSMLFLSGRDQPLAGSAVFPALEGTRPVLVEVQALIVRLQSGA
TPRRAVVGWDNGRLAMLLAVLESRCGLNFSSAEVYLNVAGGYRLSDPAADLAVAAALVSALADKPLPENSVWFGEVSLAG
EIRPVAHAGLRLREAAKLGFSTGCGPQNGGEKVGKMRYAPVDRLGKLVDRVMGTS

Sequences:

>Translated_455_residues
MAKTKRKYVCQACGTVAHRWQGQCAECAEWNSLVEDAPATVFSQKHDLSSGGRAITFVDLNQPGHELTRRTTGLAEFDRA
LGGGLVPGSAILMGGDPGIGKSTLLLQAAASIAKSGQQVVYVSGEEAAGQVRMRASRLGVADAPLKLASSTSVRDILTTL
GSMDAPALLVIDSIQTMHSDTIEGAPGTVSQVRGCAFELIRYAKESGTALVLVGHVTKDGNIAGPRVLEHMVDVVMSFEG
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVANPSMLFLSGRDQPLAGSAVFPALEGTRPVLVEVQALIVRLQSGA
TPRRAVVGWDNGRLAMLLAVLESRCGLNFSSAEVYLNVAGGYRLSDPAADLAVAAALVSALADKPLPENSVWFGEVSLAG
EIRPVAHAGLRLREAAKLGFSTGCGPQNGGEKVGKMRYAPVDRLGKLVDRVMGTS
>Mature_454_residues
AKTKRKYVCQACGTVAHRWQGQCAECAEWNSLVEDAPATVFSQKHDLSSGGRAITFVDLNQPGHELTRRTTGLAEFDRAL
GGGLVPGSAILMGGDPGIGKSTLLLQAAASIAKSGQQVVYVSGEEAAGQVRMRASRLGVADAPLKLASSTSVRDILTTLG
SMDAPALLVIDSIQTMHSDTIEGAPGTVSQVRGCAFELIRYAKESGTALVLVGHVTKDGNIAGPRVLEHMVDVVMSFEGE
RSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVANPSMLFLSGRDQPLAGSAVFPALEGTRPVLVEVQALIVRLQSGAT
PRRAVVGWDNGRLAMLLAVLESRCGLNFSSAEVYLNVAGGYRLSDPAADLAVAAALVSALADKPLPENSVWFGEVSLAGE
IRPVAHAGLRLREAAKLGFSTGCGPQNGGEKVGKMRYAPVDRLGKLVDRVMGTS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=433, Percent_Identity=49.4226327944573, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 47871; Mature: 47740

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTKRKYVCQACGTVAHRWQGQCAECAEWNSLVEDAPATVFSQKHDLSSGGRAITFVDL
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEC
NQPGHELTRRTTGLAEFDRALGGGLVPGSAILMGGDPGIGKSTLLLQAAASIAKSGQQVV
CCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEE
YVSGEEAAGQVRMRASRLGVADAPLKLASSTSVRDILTTLGSMDAPALLVIDSIQTMHSD
EECCCCCCCHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHCC
TIEGAPGTVSQVRGCAFELIRYAKESGTALVLVGHVTKDGNIAGPRVLEHMVDVVMSFEG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVANPSMLFLSGRDQPLAGSAVFPALE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCC
GTRPVLVEVQALIVRLQSGATPRRAVVGWDNGRLAMLLAVLESRCGLNFSSAEVYLNVAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
GYRLSDPAADLAVAAALVSALADKPLPENSVWFGEVSLAGEIRPVAHAGLRLREAAKLGF
CCEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
STGCGPQNGGEKVGKMRYAPVDRLGKLVDRVMGTS
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTKRKYVCQACGTVAHRWQGQCAECAEWNSLVEDAPATVFSQKHDLSSGGRAITFVDL
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEC
NQPGHELTRRTTGLAEFDRALGGGLVPGSAILMGGDPGIGKSTLLLQAAASIAKSGQQVV
CCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEE
YVSGEEAAGQVRMRASRLGVADAPLKLASSTSVRDILTTLGSMDAPALLVIDSIQTMHSD
EECCCCCCCHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHCC
TIEGAPGTVSQVRGCAFELIRYAKESGTALVLVGHVTKDGNIAGPRVLEHMVDVVMSFEG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVANPSMLFLSGRDQPLAGSAVFPALE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCC
GTRPVLVEVQALIVRLQSGATPRRAVVGWDNGRLAMLLAVLESRCGLNFSSAEVYLNVAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
GYRLSDPAADLAVAAALVSALADKPLPENSVWFGEVSLAGEIRPVAHAGLRLREAAKLGF
CCEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
STGCGPQNGGEKVGKMRYAPVDRLGKLVDRVMGTS
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA