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The map label for this gene is grpE

Identifier: 85374031

GI number: 85374031

Start: 1245346

End: 1245939

Strand: Direct

Name: grpE

Synonym: ELI_06020

Alternate gene names: 85374031

Gene position: 1245346-1245939 (Clockwise)

Preceding gene: 85374030

Following gene: 85374033

Centisome position: 40.8

GC content: 62.96

Gene sequence:

>594_bases
ATGAGCGACGATACCAAGAAGCAGGACACTGCGGCTGACGCAGAAGTCGAGAAGGAAATGGAAGGCGTTCCCGAGCATCT
ACGCGATGACCGTGGGAGCGAAGAGGACGCGTCGGACGACCTGTCCGCAGCACTTGAATCGCTAAAGAGCGATCTCGAGG
CGGCCAAGCAGGAAACGCTCTACGCCAAGGCCGAGACGCAAAACGTCCGGCGCCGGATGGAGAAGGACATCCAGGACGCG
CGCACCTATGCCGCGACCGGTTTTGCGCGCGATATCCTGTCCATCGCAGACAACCTGGCGCGCGCGATCGATGCCATTCC
GCAGGAGCTGCGCGAAGACGAGAAGTTCAAGGGACTGGTCGCCGGTATCGAGGCCACGCAGCGCGAACTCGACAAGGTCT
TTGCGCAGCACGGCGTCAGCCGCATCGCCGCCATGGGCCTGCCGCTCGACCCCAACCAGCATCAGGCGATGATGGAAGTC
CCGACCGACGAAGTCGAACCGGGCACCATCGTGCAGGAAATGCAGGCGGGCTACATGATCCGCGACCGCCTGCTGCGCCC
GTCCATGGTGGGTGTCGCAAAGAAGCCGGACTGA

Upstream 100 bases:

>100_bases
GGCCCCACGCGGTTGAATTACGCGCGCGTCGTCCCCATGGTGGATTTCACGGCCCGTTCGCTGGGCAAACTGATCGGTTA
ACCGGAAAAATTTCAAAGCA

Downstream 100 bases:

>100_bases
TCGGGGCGGTTAGAGCTTAGGTAGCTCCGTTGCCTCGATGGCCGCGATGACCTCCGCATTCACCTGCGCCTTGTTACCGG
CGAAGGCGGGCAGCTTCATG

Product: molecular chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSDDTKKQDTAADAEVEKEMEGVPEHLRDDRGSEEDASDDLSAALESLKSDLEAAKQETLYAKAETQNVRRRMEKDIQDA
RTYAATGFARDILSIADNLARAIDAIPQELREDEKFKGLVAGIEATQRELDKVFAQHGVSRIAAMGLPLDPNQHQAMMEV
PTDEVEPGTIVQEMQAGYMIRDRLLRPSMVGVAKKPD

Sequences:

>Translated_197_residues
MSDDTKKQDTAADAEVEKEMEGVPEHLRDDRGSEEDASDDLSAALESLKSDLEAAKQETLYAKAETQNVRRRMEKDIQDA
RTYAATGFARDILSIADNLARAIDAIPQELREDEKFKGLVAGIEATQRELDKVFAQHGVSRIAAMGLPLDPNQHQAMMEV
PTDEVEPGTIVQEMQAGYMIRDRLLRPSMVGVAKKPD
>Mature_196_residues
SDDTKKQDTAADAEVEKEMEGVPEHLRDDRGSEEDASDDLSAALESLKSDLEAAKQETLYAKAETQNVRRRMEKDIQDAR
TYAATGFARDILSIADNLARAIDAIPQELREDEKFKGLVAGIEATQRELDKVFAQHGVSRIAAMGLPLDPNQHQAMMEVP
TDEVEPGTIVQEMQAGYMIRDRLLRPSMVGVAKKPD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=178, Percent_Identity=33.1460674157303, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI40255109, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788967, Length=135, Percent_Identity=39.2592592592593, Blast_Score=97, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324806, Length=179, Percent_Identity=31.8435754189944, Blast_Score=106, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_ERYLH (Q2NAJ5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458093.1
- STRING:   Q2NAJ5
- GeneID:   3870755
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06020
- NMPDR:   fig|314225.3.peg.1856
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   Q2NAJ5
- ProtClustDB:   CLSK835067
- BioCyc:   ELIT314225:ELI_06020-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21842; Mature: 21710

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDTKKQDTAADAEVEKEMEGVPEHLRDDRGSEEDASDDLSAALESLKSDLEAAKQETL
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YAKAETQNVRRRMEKDIQDARTYAATGFARDILSIADNLARAIDAIPQELREDEKFKGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIEATQRELDKVFAQHGVSRIAAMGLPLDPNQHQAMMEVPTDEVEPGTIVQEMQAGYMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
RDRLLRPSMVGVAKKPD
HHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
SDDTKKQDTAADAEVEKEMEGVPEHLRDDRGSEEDASDDLSAALESLKSDLEAAKQETL
CCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YAKAETQNVRRRMEKDIQDARTYAATGFARDILSIADNLARAIDAIPQELREDEKFKGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIEATQRELDKVFAQHGVSRIAAMGLPLDPNQHQAMMEVPTDEVEPGTIVQEMQAGYMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
RDRLLRPSMVGVAKKPD
HHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA