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The map label for this gene is pyrC

Identifier: 85373397

GI number: 85373397

Start: 600639

End: 601673

Strand: Direct

Name: pyrC

Synonym: ELI_02850

Alternate gene names: 85373397

Gene position: 600639-601673 (Clockwise)

Preceding gene: 85373395

Following gene: 85373399

Centisome position: 19.68

GC content: 62.9

Gene sequence:

>1035_bases
ATGACCGAGACGCTGACGATCCGCCGCCCCGACGACTGGCACCTCCATTTCCGCGACGGCGCGATGATGCGCGGCGTCGT
GCCCTATACCGCGCGCCAGTTCGCCCGCGCGATCGTAATGCCCAACCTGACCCCTCCGGTAACGACGACAGCGCTGGGGG
CTGCCTATCGCGAGCGGATCCTCGCTGCCGTGCCGGAGGGCGTTGATTTTAGGCCGCTGATGACCGCCTATCTCACCGAC
GAAACCGACGCCGACGATCTCATCGACGGTTACACGCACGGCGTGTTTACGGCTGCCAAGCTTTACCCCGCCAATGCCAC
GACCAATTCTGCGTCGGGCGTGACCGATGTCGAAGCGCTCTATCCGGTGTTCGAGCGGATGGCAGCAGCTGGCATGGTCC
TGTGCATTCACGGCGAGGTTACCGACGCCGACGTCGACGTGTTCGACCGCGAGAAAGAGTTCATCGAGCGGACCATGGCA
CCGCTTCACGCCGCGATCCCGGCGCTCAAGATCGCGTTCGAGCATATCACGACCAGCGATGCGGTCGATTTCGTAGTCGG
CGCAAACGACAATATCGGCGCGACCATCACGCCGCAGCACCTGCACATCAACCGTAATGCCATGCTGGTCGGCGGCATCC
AGCCGCATAATTACTGCCTGCCGGTCGCCAAGCGCGAGACGCATCGGCTGGCGCTACGCCAGGCGGCGACCTCCGGTCCG
CCCAAATTCTTCCTCGGCACCGACAGCGCGCCGCATGAAAAGCACACCAAGGAAAGCGCCTGCGGCTGCGCGGGCATCTT
CGGCGCGCCCTATGCGCTGGAGAGCTATCTCGCGGTGTTCGAAGAGGAAGATGCGCTTGATAAGTTCGAAGCCTTCGCCT
CGCTCAACGGTCCTGCGTTTTATGGCCTGCCGGTCAATGACGAGACAATCACGCTGGAGCGGGTGCCGCATAATGTGCCC
GATTCTCTGGAGACGGAAGGCGGCAAGGTCGTGCCATTCCACGCCGGGCAGGAGCTTAACTGGCGGCTTAAATGA

Upstream 100 bases:

>100_bases
CGTACCAGGGCAGGTCAGGAAGCCGATATGCATGCGAGCGCCCTTAGGCGTGCGATTGCCTGCTGGAAAGATGCGGCGTG
CCGCACTAGAGCAGCGCGCG

Downstream 100 bases:

>100_bases
GCTCTCGTGAAACCAGGAATCTCGGAAATAGGCGACCAGCTAGCGGCCTGAGGCCCCCGCCTTCGCGGGGGCTCTGCTCT
TCGCCCACATGTCCCACACG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTETLTIRRPDDWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTD
ETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMA
PLHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGP
PKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLPVNDETITLERVPHNVP
DSLETEGGKVVPFHAGQELNWRLK

Sequences:

>Translated_344_residues
MTETLTIRRPDDWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTD
ETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMA
PLHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGP
PKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLPVNDETITLERVPHNVP
DSLETEGGKVVPFHAGQELNWRLK
>Mature_343_residues
TETLTIRRPDDWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTDE
TDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAP
LHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGPP
KFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLPVNDETITLERVPHNVPD
SLETEGGKVVPFHAGQELNWRLK

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=343, Percent_Identity=60.0583090379009, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323452, Length=370, Percent_Identity=32.4324324324324, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_ERYLH (Q2NCC9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457459.1
- ProteinModelPortal:   Q2NCC9
- SMR:   Q2NCC9
- STRING:   Q2NCC9
- GeneID:   3869096
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02850
- NMPDR:   fig|314225.3.peg.2511
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q2NCC9
- ProtClustDB:   PRK05451
- BioCyc:   ELIT314225:ELI_02850-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37576; Mature: 37445

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLTIRRPDDWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERI
CCCEEEEECCCCCEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LAAVPEGVDFRPLMTAYLTDETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEAL
HHHCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHH
YPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAPLHAAIPALKIAFEHITTSD
HHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGP
CEEEEEECCCCCCCEECCCEEEECCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCC
PKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAF
CEEEEECCCCCCHHHCHHHHCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
YGLPVNDETITLERVPHNVPDSLETEGGKVVPFHAGQELNWRLK
EEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEC
>Mature Secondary Structure 
TETLTIRRPDDWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERI
CCEEEEECCCCCEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LAAVPEGVDFRPLMTAYLTDETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEAL
HHHCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHH
YPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAPLHAAIPALKIAFEHITTSD
HHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGP
CEEEEEECCCCCCCEECCCEEEECCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCC
PKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAF
CEEEEECCCCCCHHHCHHHHCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
YGLPVNDETITLERVPHNVPDSLETEGGKVVPFHAGQELNWRLK
EEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA