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The map label for this gene is moaE [H]

Identifier: 85373385

GI number: 85373385

Start: 592186

End: 592656

Strand: Direct

Name: moaE [H]

Synonym: ELI_02790

Alternate gene names: 85373385

Gene position: 592186-592656 (Clockwise)

Preceding gene: 85373384

Following gene: 85373386

Centisome position: 19.4

GC content: 64.54

Gene sequence:

>471_bases
GTGGCTAGGCTCACGCTCAAGACTCCCGCCGATGTGCGGCTGCTCGACAAGGGCCTGTCGGCAGGCGAGGCGTTGGCCGC
GTTCAATGCCAGCAATCCCGAAGCGGGGGCTATCGCCACGTTCGCAGGCAAGGTTCGTCCCGATGGCGGGGTGCAGGCGC
TGGAGCTGTCGCATTACGAACTGCTCACGCTCCCGGGCATGAAGAAACTTGCGGCCGATGCGCTGACGCGGTTCGAATGC
GCCGGGCTGCTCATCTGGCACCGTGTCGGTGTCATGACACCGGGCGAAACGATCGTGTTGGTCGCCGCCGCCGCGCCCCA
TCGTCGTGCCGCTTTCGAAGCGGTCGATTTCTGCATGGATTATCTCAAGAGTGCGTCCTGGTTCTGGAAGCGCGAGAAGC
GCGGCGGCGAATGGCACTGGATCGAGCCGCGCCCCGAGGATTACGCCGACCGCAAACGCTGGGAGAGATAA

Upstream 100 bases:

>100_bases
GACCGCGTCAATGTCGCCTGTGCGGGCACGGTGCTCGCTGATAAAACCGCGCTAGCCGCGCTGGACGGCGACGAGGTCGC
GCTGTTGCCACCTGTCAGCG

Downstream 100 bases:

>100_bases
TCTACGCCATTTGATTCAGATCAAAGCTGCGCGAATCGAACCCTGCCACAAGACCTTCAAGACAAGAAGGAGTGGCCCAA
TGTCCGAACTGCTGACCCGT

Product: molybdopterin converting factor, subunit 2

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MARLTLKTPADVRLLDKGLSAGEALAAFNASNPEAGAIATFAGKVRPDGGVQALELSHYELLTLPGMKKLAADALTRFEC
AGLLIWHRVGVMTPGETIVLVAAAAPHRRAAFEAVDFCMDYLKSASWFWKREKRGGEWHWIEPRPEDYADRKRWER

Sequences:

>Translated_156_residues
MARLTLKTPADVRLLDKGLSAGEALAAFNASNPEAGAIATFAGKVRPDGGVQALELSHYELLTLPGMKKLAADALTRFEC
AGLLIWHRVGVMTPGETIVLVAAAAPHRRAAFEAVDFCMDYLKSASWFWKREKRGGEWHWIEPRPEDYADRKRWER
>Mature_155_residues
ARLTLKTPADVRLLDKGLSAGEALAAFNASNPEAGAIATFAGKVRPDGGVQALELSHYELLTLPGMKKLAADALTRFECA
GLLIWHRVGVMTPGETIVLVAAAAPHRRAAFEAVDFCMDYLKSASWFWKREKRGGEWHWIEPRPEDYADRKRWER

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Escherichia coli, GI1787003, Length=128, Percent_Identity=39.84375, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 17349; Mature: 17218

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLTLKTPADVRLLDKGLSAGEALAAFNASNPEAGAIATFAGKVRPDGGVQALELSHYE
CCEEECCCCCCHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCEE
LLTLPGMKKLAADALTRFECAGLLIWHRVGVMTPGETIVLVAAAAPHRRAAFEAVDFCMD
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
YLKSASWFWKREKRGGEWHWIEPRPEDYADRKRWER
HHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHCCC
>Mature Secondary Structure 
ARLTLKTPADVRLLDKGLSAGEALAAFNASNPEAGAIATFAGKVRPDGGVQALELSHYE
CEEECCCCCCHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCEE
LLTLPGMKKLAADALTRFECAGLLIWHRVGVMTPGETIVLVAAAAPHRRAAFEAVDFCMD
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
YLKSASWFWKREKRGGEWHWIEPRPEDYADRKRWER
HHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]