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The map label for this gene is moaA

Identifier: 85373383

GI number: 85373383

Start: 590951

End: 591949

Strand: Direct

Name: moaA

Synonym: ELI_02780

Alternate gene names: 85373383

Gene position: 590951-591949 (Clockwise)

Preceding gene: 85373382

Following gene: 85373384

Centisome position: 19.36

GC content: 64.66

Gene sequence:

>999_bases
ATGGCAGCAAGCGAACTGATCGACACATTCGGACGGCGCATTTCCTATCTGCGCCTGTCGGTGACCGATCGCTGCGACCT
GCGCTGCACCTATTGCATGCCCGAGCGGATGCAGTTCCTGCCGCGCGCCGAAGTGCTGACGCTGGAAGAGCTGCGCGACC
TCGCCGTCGGCTTCATGCGGCGCGGCATCACCAAGATCCGCCTGACCGGCGGCGAACCGCTGGTACGGCGCGATGTCGTG
GACCTGATCCGTGCGTTGGGACGACATGTCGGAGACGGGCTGGAAGAACTGACCCTCACCACCAACGGCACGCAACTGGC
AGGCCATGCCCAGGCGATCGCCGATGCCGGTGTGAAGCGGATCAATATCTCGCTCGACACGCTGGATCGCGCGACCTTCC
AGCGCCTCTCGCGCCGCGATGCCTTGGCGAGCGTGCTCGGTGGGATCGCCGCGGCCAAGGCGGCGGGTCTCAAGATCAAA
ATCAATACCGTCGCGATGAAAGGCGTGAACGGGCACGAAATCCCTGCGCTGATCGAATGGGCGCATGGCGAGGGACACGA
CGTGACCCTGATCGAGACGATGCCGCTCGGCGAGGTGGACGAGGATCGGACCGACCAGTTCCTGCCACTCGTAGCTGTAC
GCGAGTCGCTGGAAGCGCGCTGGACCCTCACGGACAGCGACCATCGCACCGGCGGCCCCTCGCGCTATGTCGAGGTCGCT
GAAACGGGTGGCAGGCTCGGCTTCATCACCCCGCTCACCAACAATTTCTGCGCTGGCTGCAACCGCGTCCGCGTGACCGC
GACCGGTCAGCTTTATCCCTGCCTTGGAGGGGGAGAACGCGTGGACCTGCGCGCCGCGCTGCGCTCCGACGATCCCGATA
GCAATCTATCGACCGCGCTCGATACCGCGATGAAAATCAAACCCGAGCGGCATGACTTCCGTATCGGCGAAGGCGAAGAG
CCCGCGCTAGCACGCCATATGTCCATGACCGGGGGCTGA

Upstream 100 bases:

>100_bases
GGCCTATTGCGACAAAGCCCGGGGTGTAACCGGACACTATCTTCCGCTGAATAGCCTAGGGGCGGGTGACCCGCTCGCGA
GCAGCGGTTAGAGTGAGGCC

Downstream 100 bases:

>100_bases
GATGGCCATCACCATCCTCTTCCTCGGCCCCTTGCGCGATCTGGCGGGCGAGCAGGAGCGCGAAGTCGCCGCACCGCTTG
ACTGGCAGGGGCTCTTGGAA

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAASELIDTFGRRISYLRLSVTDRCDLRCTYCMPERMQFLPRAEVLTLEELRDLAVGFMRRGITKIRLTGGEPLVRRDVV
DLIRALGRHVGDGLEELTLTTNGTQLAGHAQAIADAGVKRINISLDTLDRATFQRLSRRDALASVLGGIAAAKAAGLKIK
INTVAMKGVNGHEIPALIEWAHGEGHDVTLIETMPLGEVDEDRTDQFLPLVAVRESLEARWTLTDSDHRTGGPSRYVEVA
ETGGRLGFITPLTNNFCAGCNRVRVTATGQLYPCLGGGERVDLRAALRSDDPDSNLSTALDTAMKIKPERHDFRIGEGEE
PALARHMSMTGG

Sequences:

>Translated_332_residues
MAASELIDTFGRRISYLRLSVTDRCDLRCTYCMPERMQFLPRAEVLTLEELRDLAVGFMRRGITKIRLTGGEPLVRRDVV
DLIRALGRHVGDGLEELTLTTNGTQLAGHAQAIADAGVKRINISLDTLDRATFQRLSRRDALASVLGGIAAAKAAGLKIK
INTVAMKGVNGHEIPALIEWAHGEGHDVTLIETMPLGEVDEDRTDQFLPLVAVRESLEARWTLTDSDHRTGGPSRYVEVA
ETGGRLGFITPLTNNFCAGCNRVRVTATGQLYPCLGGGERVDLRAALRSDDPDSNLSTALDTAMKIKPERHDFRIGEGEE
PALARHMSMTGG
>Mature_331_residues
AASELIDTFGRRISYLRLSVTDRCDLRCTYCMPERMQFLPRAEVLTLEELRDLAVGFMRRGITKIRLTGGEPLVRRDVVD
LIRALGRHVGDGLEELTLTTNGTQLAGHAQAIADAGVKRINISLDTLDRATFQRLSRRDALASVLGGIAAAKAAGLKIKI
NTVAMKGVNGHEIPALIEWAHGEGHDVTLIETMPLGEVDEDRTDQFLPLVAVRESLEARWTLTDSDHRTGGPSRYVEVAE
TGGRLGFITPLTNNFCAGCNRVRVTATGQLYPCLGGGERVDLRAALRSDDPDSNLSTALDTAMKIKPERHDFRIGEGEEP
ALARHMSMTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=335, Percent_Identity=38.2089552238806, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI22027536, Length=335, Percent_Identity=38.2089552238806, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1786999, Length=333, Percent_Identity=34.8348348348348, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566331, Length=336, Percent_Identity=36.0119047619048, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17561210, Length=315, Percent_Identity=36.1904761904762, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=315, Percent_Identity=35.5555555555556, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28574490, Length=313, Percent_Identity=37.3801916932907, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574488, Length=313, Percent_Identity=37.3801916932907, Blast_Score=198, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_ERYLH (Q2NCE3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457445.1
- HSSP:   P69848
- ProteinModelPortal:   Q2NCE3
- SMR:   Q2NCE3
- STRING:   Q2NCE3
- GeneID:   3869082
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02780
- NMPDR:   fig|314225.3.peg.2527
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   SRHMSTT
- PhylomeDB:   Q2NCE3
- ProtClustDB:   CLSK746369
- BioCyc:   ELIT314225:ELI_02780-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36256; Mature: 36125

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01305 MOAA_NIFB_PQQE; PS00216 SUGAR_TRANSPORT_1

Important sites: BINDING 18-18 BINDING 31-31 BINDING 67-67 BINDING 71-71 BINDING 100-100 BINDING 124-124 BINDING 160-160 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASELIDTFGRRISYLRLSVTDRCDLRCTYCMPERMQFLPRAEVLTLEELRDLAVGFMR
CCHHHHHHHHHHHEEEEEEEECCCCCCEEEECCHHHHHHCCCCCEEEHHHHHHHHHHHHH
RGITKIRLTGGEPLVRRDVVDLIRALGRHVGDGLEELTLTTNGTQLAGHAQAIADAGVKR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEECHHHHHHHHCCCEE
INISLDTLDRATFQRLSRRDALASVLGGIAAAKAAGLKIKINTVAMKGVNGHEIPALIEW
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCEEEEE
AHGEGHDVTLIETMPLGEVDEDRTDQFLPLVAVRESLEARWTLTDSDHRTGGPSRYVEVA
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHEEEEE
ETGGRLGFITPLTNNFCAGCNRVRVTATGQLYPCLGGGERVDLRAALRSDDPDSNLSTAL
CCCCCEEEECCCCCCHHCCCCEEEEEECCEEEEECCCCCEEEEEEECCCCCCCCHHHHHH
DTAMKIKPERHDFRIGEGEEPALARHMSMTGG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AASELIDTFGRRISYLRLSVTDRCDLRCTYCMPERMQFLPRAEVLTLEELRDLAVGFMR
CHHHHHHHHHHHEEEEEEEECCCCCCEEEECCHHHHHHCCCCCEEEHHHHHHHHHHHHH
RGITKIRLTGGEPLVRRDVVDLIRALGRHVGDGLEELTLTTNGTQLAGHAQAIADAGVKR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEECHHHHHHHHCCCEE
INISLDTLDRATFQRLSRRDALASVLGGIAAAKAAGLKIKINTVAMKGVNGHEIPALIEW
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCEEEEE
AHGEGHDVTLIETMPLGEVDEDRTDQFLPLVAVRESLEARWTLTDSDHRTGGPSRYVEVA
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHEEEEE
ETGGRLGFITPLTNNFCAGCNRVRVTATGQLYPCLGGGERVDLRAALRSDDPDSNLSTAL
CCCCCEEEECCCCCCHHCCCCEEEEEECCEEEEECCCCCEEEEEEECCCCCCCCHHHHHH
DTAMKIKPERHDFRIGEGEEPALARHMSMTGG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA