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The map label for this gene is iscS2 [H]

Identifier: 85373265

GI number: 85373265

Start: 478606

End: 479529

Strand: Direct

Name: iscS2 [H]

Synonym: ELI_02190

Alternate gene names: 85373265

Gene position: 478606-479529 (Clockwise)

Preceding gene: 85373262

Following gene: 85373266

Centisome position: 15.68

GC content: 62.66

Gene sequence:

>924_bases
ATGGAGGAAGGCTTCCGCATCTGGGCCAACCCTTCGAGCCCGCATGCCGAAGGGCGCAAGGCCAAGGCTGCGCTGGAAGA
TGCGCGCGAGCGGGTGAAGCGCGCGCTCGGCTGGGACGGCGAAGTGATCTTCACCAGCGGGGCGAGCGAGGCGCTGGCGC
TCGGCCTGCAGCGCTGCAAGGCGAAGCGCCGGATCGTCAGCGCAGTCGAGCATGATGCGGTATTCCGCGCGGCGCCGGAT
GCGCAGGTCTATTCGGATGATATCACAATCGGCGCCGATGACATTCTGGCCATCCAACACGTCAATTCGGAAACGGGCGT
AGTCAACCCGGTCGGGCAGATGATCGAGACGATCAGGCCGACAGGAGCGCTTGTACTGTCCGATTGCTCCCAATCGGCGG
GTAAGCGCGATTTGCCGAATGCCGACATGCTCGTGATATCGGCGCACAAATTCGGCGGGCCTATCGGTATCGGCGCACTG
CTGGTGCGGGATTTTGCGCTGCTGGAGCCGATGGGAGGGCACGAGCGAGGCTATCGGCAGGGTACGGAGAACGTGGCGGG
CGCTATAGGGATGGCGGCTGCGCTCGAGGCCGGGCCATGGAACACCAGCTCGAAAGAACGGAAAAATTTCGCCCTCAGCC
TCGGAAACCCAGTGCTTTCGTTCGGCGAGCAGGCAGACTACATCTTCGCGCTCGCCCACCCGACAATGAGCGCTCAGGCC
TTGCTCATTCGGCTCGACGCAATGGGCTTTGCTGTATCGGCGGGCAGCGCGTGTTCCTCTGGAACGCTCAAGAAGAGTCG
AGTGCTTGATGCCTTTGGTGTCGATGATGACACCGCCAGCCGCACCATCCGTGTCAGCATTGGTTGGAATACGACGAGCG
AGGAACTCGAACGCTTTGCCGAGGCCTGGCGCAGCCTGGCTTAG

Upstream 100 bases:

>100_bases
AATCGCAATCATCGTAACCATGGGGTCATAGCCCATTCCCGACCGCATCTATCTCGACCACGCAGCCACCACCCCGCTGC
GCCCAGAAGCGAAGGCGGCG

Downstream 100 bases:

>100_bases
CCCGTTCGCCCTGAGCTTGTCGGAAGCGAAGCTGACGCGCAGCGTCGACGGCTGTACTTTCTTGCTGTTCCGCGTCCGCC
AAAAGGAAGAACAGGGCTTC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEEGFRIWANPSSPHAEGRKAKAALEDARERVKRALGWDGEVIFTSGASEALALGLQRCKAKRRIVSAVEHDAVFRAAPD
AQVYSDDITIGADDILAIQHVNSETGVVNPVGQMIETIRPTGALVLSDCSQSAGKRDLPNADMLVISAHKFGGPIGIGAL
LVRDFALLEPMGGHERGYRQGTENVAGAIGMAAALEAGPWNTSSKERKNFALSLGNPVLSFGEQADYIFALAHPTMSAQA
LLIRLDAMGFAVSAGSACSSGTLKKSRVLDAFGVDDDTASRTIRVSIGWNTTSEELERFAEAWRSLA

Sequences:

>Translated_307_residues
MEEGFRIWANPSSPHAEGRKAKAALEDARERVKRALGWDGEVIFTSGASEALALGLQRCKAKRRIVSAVEHDAVFRAAPD
AQVYSDDITIGADDILAIQHVNSETGVVNPVGQMIETIRPTGALVLSDCSQSAGKRDLPNADMLVISAHKFGGPIGIGAL
LVRDFALLEPMGGHERGYRQGTENVAGAIGMAAALEAGPWNTSSKERKNFALSLGNPVLSFGEQADYIFALAHPTMSAQA
LLIRLDAMGFAVSAGSACSSGTLKKSRVLDAFGVDDDTASRTIRVSIGWNTTSEELERFAEAWRSLA
>Mature_307_residues
MEEGFRIWANPSSPHAEGRKAKAALEDARERVKRALGWDGEVIFTSGASEALALGLQRCKAKRRIVSAVEHDAVFRAAPD
AQVYSDDITIGADDILAIQHVNSETGVVNPVGQMIETIRPTGALVLSDCSQSAGKRDLPNADMLVISAHKFGGPIGIGAL
LVRDFALLEPMGGHERGYRQGTENVAGAIGMAAALEAGPWNTSSKERKNFALSLGNPVLSFGEQADYIFALAHPTMSAQA
LLIRLDAMGFAVSAGSACSSGTLKKSRVLDAFGVDDDTASRTIRVSIGWNTTSEELERFAEAWRSLA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=342, Percent_Identity=29.5321637426901, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI48994898, Length=346, Percent_Identity=31.5028901734104, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=26.1494252873563, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=341, Percent_Identity=29.6187683284457, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319831, Length=343, Percent_Identity=30.3206997084548, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI20129463, Length=344, Percent_Identity=32.2674418604651, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 32666; Mature: 32666

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEGFRIWANPSSPHAEGRKAKAALEDARERVKRALGWDGEVIFTSGASEALALGLQRCK
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
AKRRIVSAVEHDAVFRAAPDAQVYSDDITIGADDILAIQHVNSETGVVNPVGQMIETIRP
HHHHHHHHHHHHHHEECCCCCEEECCCEEECCCCEEEEEECCCCCCCCCHHHHHHHHHCC
TGALVLSDCSQSAGKRDLPNADMLVISAHKFGGPIGIGALLVRDFALLEPMGGHERGYRQ
CCCEEEEHHHHCCCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHC
GTENVAGAIGMAAALEAGPWNTSSKERKNFALSLGNPVLSFGEQADYIFALAHPTMSAQA
CHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEECCCHHHHCCCCCCEEEEEECCCCCHHE
LLIRLDAMGFAVSAGSACSSGTLKKSRVLDAFGVDDDTASRTIRVSIGWNTTSEELERFA
EEEEEEHHCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHH
EAWRSLA
HHHHHCC
>Mature Secondary Structure
MEEGFRIWANPSSPHAEGRKAKAALEDARERVKRALGWDGEVIFTSGASEALALGLQRCK
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
AKRRIVSAVEHDAVFRAAPDAQVYSDDITIGADDILAIQHVNSETGVVNPVGQMIETIRP
HHHHHHHHHHHHHHEECCCCCEEECCCEEECCCCEEEEEECCCCCCCCCHHHHHHHHHCC
TGALVLSDCSQSAGKRDLPNADMLVISAHKFGGPIGIGALLVRDFALLEPMGGHERGYRQ
CCCEEEEHHHHCCCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHC
GTENVAGAIGMAAALEAGPWNTSSKERKNFALSLGNPVLSFGEQADYIFALAHPTMSAQA
CHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEECCCHHHHCCCCCCEEEEEECCCCCHHE
LLIRLDAMGFAVSAGSACSSGTLKKSRVLDAFGVDDDTASRTIRVSIGWNTTSEELERFA
EEEEEEHHCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHH
EAWRSLA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]