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The map label for this gene is gatA

Identifier: 85373102

GI number: 85373102

Start: 309184

End: 310671

Strand: Direct

Name: gatA

Synonym: ELI_01375

Alternate gene names: 85373102

Gene position: 309184-310671 (Clockwise)

Preceding gene: 85373101

Following gene: 85373103

Centisome position: 10.13

GC content: 66.26

Gene sequence:

>1488_bases
ATGAGTAGTGAACTAACCGAACTCGGCGTCGCAGACATCCGCGACGGGGTTGCCGATGGCAGCTTCACCGCCCGCGAAGT
GGCGGAGGCCTTCAACGCGCAGGTGGCCGCAGCCAAGGACCTCGGCGCTTTTATCGTCGAGACGCCGGAAAAGGCGCTCG
AAGCCGCCGCTGCGGTCGATGCGGCGCGCGCGAATGGCGAAACGCTCGGCAGCATGGCCGGGGTGCCGATCGGCATGAAG
GACCTGTTCTGCACCGACGGCGTGCAGACCACCGCAGCCAGCCACATCCTCGAAGGCTTCGTGCCGCAGTACGAGTCGAC
CGTCAGCGCCAACCTGTGGCGCGCCGGTGCGGGCATGCTGGGCAAGCTCAACATGGACCAGTTCGCGATGGGCTCGTCCA
ACGAGACATCCTATTTCGGCCCGGTGAAGAGCCCGTGGCGGAAAGCCGGGAGCAACACTGCGCTGACGCCGGGCGGCTCT
TCTGGTGGCTCCAGTTCCGCTGTTGCTGCGCGGCTGTGCCCGGCCGCGACCGGTACCGACACCGGCGGCTCGATCCGCCA
GCCCGCCGCGATCACCGGCACGACCGGGATCAAGCCGACCTACGGCCGTTGCAGCCGCTGGGGCGTCGTGGCCTTTGCCA
GCAGCCTCGACCAGGCCGGCCCGATAGCGCGCAGCGTCCGGGATTGCGCGATCATGCTCGAGGCCATGGCGGGTTTCGAT
CCCAAGGATGCGACCAGCCTGGATATGCCTGTGCCCCAGTGGGAAGCGGGCCTTTCTGCGGACCTCAGCGGCAAGAAGAT
CGGCATTCCGCGCGAATACCGCATGGACGGCACCCCGCAGGATATTCTCGACAGCTGGGAACGCGGCAAGAAATGGCTTA
AGGATGCAGGCGCAGAGATCGTCGATATCTCGCTGCCGCACACCAAATATGCGCTGCCCGCCTATTACATCATCGCGCCG
GCCGAAGCTTCCAGCAACCTCGCGCGCTATGACGGCGTGCGTTACGGCCTGCGCGACCTGCCCGATGGCGCGGGACTGCA
AGACATGTATGCCGCCACTCGCGAGGAAGGCTTCGGCGACGAGGTCAAGCGCCGCATCCTGATCGGCACCTATGTGCTGA
GCGCGGGCTTCTACGACGCCTATTACAACCAGGCGCAGAAGATCCGCACGCTGGTCGCGCGCGACTTCGAGCAGGCTTGG
GCCGAATGCGACCTGATCCTCGCCCCGACCACGCCCAATGCGGCTTTCGGCCTCGGCGAAATGCAGGCCGATCCGCTGGC
GATGTATCTCAACGATGTCTTCGCCGTCCCCGCCTCGCTCGCAGGCCTGCCCGCGATAAGCGTGCCGGGCGGTCTGAACT
CGGACGGCCTTCCGCTCGGCCTGCAACTGGTCGGCAAGCCCTTCGACGAGCAGGGCGTGCTAAACGCCGGTCTCGCCATC
GAGCAGCGCAATGGGTTCACCGCCAGGCCGGAGACATGGTGGGCGTGA

Upstream 100 bases:

>100_bases
GTTTTCATTCAATCATCCCCGACAGCGGCGCAGCCGCGACCGATCGGGGATCCAGAAACAAAGGGAAGCTGGATTCCCGC
CTGCGCGGGAATGACGAAGT

Downstream 100 bases:

>100_bases
CTTCGTCGAGCGTTGGGCCGAGTTTCGGGATTGTTTATACGGTCAAGAACGAGGCCCGGCTGCTACCCGATGCGCTGAAG
TGGCATCGGACGCTTGGTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSSELTELGVADIRDGVADGSFTAREVAEAFNAQVAAAKDLGAFIVETPEKALEAAAAVDAARANGETLGSMAGVPIGMK
DLFCTDGVQTTAASHILEGFVPQYESTVSANLWRAGAGMLGKLNMDQFAMGSSNETSYFGPVKSPWRKAGSNTALTPGGS
SGGSSSAVAARLCPAATGTDTGGSIRQPAAITGTTGIKPTYGRCSRWGVVAFASSLDQAGPIARSVRDCAIMLEAMAGFD
PKDATSLDMPVPQWEAGLSADLSGKKIGIPREYRMDGTPQDILDSWERGKKWLKDAGAEIVDISLPHTKYALPAYYIIAP
AEASSNLARYDGVRYGLRDLPDGAGLQDMYAATREEGFGDEVKRRILIGTYVLSAGFYDAYYNQAQKIRTLVARDFEQAW
AECDLILAPTTPNAAFGLGEMQADPLAMYLNDVFAVPASLAGLPAISVPGGLNSDGLPLGLQLVGKPFDEQGVLNAGLAI
EQRNGFTARPETWWA

Sequences:

>Translated_495_residues
MSSELTELGVADIRDGVADGSFTAREVAEAFNAQVAAAKDLGAFIVETPEKALEAAAAVDAARANGETLGSMAGVPIGMK
DLFCTDGVQTTAASHILEGFVPQYESTVSANLWRAGAGMLGKLNMDQFAMGSSNETSYFGPVKSPWRKAGSNTALTPGGS
SGGSSSAVAARLCPAATGTDTGGSIRQPAAITGTTGIKPTYGRCSRWGVVAFASSLDQAGPIARSVRDCAIMLEAMAGFD
PKDATSLDMPVPQWEAGLSADLSGKKIGIPREYRMDGTPQDILDSWERGKKWLKDAGAEIVDISLPHTKYALPAYYIIAP
AEASSNLARYDGVRYGLRDLPDGAGLQDMYAATREEGFGDEVKRRILIGTYVLSAGFYDAYYNQAQKIRTLVARDFEQAW
AECDLILAPTTPNAAFGLGEMQADPLAMYLNDVFAVPASLAGLPAISVPGGLNSDGLPLGLQLVGKPFDEQGVLNAGLAI
EQRNGFTARPETWWA
>Mature_494_residues
SSELTELGVADIRDGVADGSFTAREVAEAFNAQVAAAKDLGAFIVETPEKALEAAAAVDAARANGETLGSMAGVPIGMKD
LFCTDGVQTTAASHILEGFVPQYESTVSANLWRAGAGMLGKLNMDQFAMGSSNETSYFGPVKSPWRKAGSNTALTPGGSS
GGSSSAVAARLCPAATGTDTGGSIRQPAAITGTTGIKPTYGRCSRWGVVAFASSLDQAGPIARSVRDCAIMLEAMAGFDP
KDATSLDMPVPQWEAGLSADLSGKKIGIPREYRMDGTPQDILDSWERGKKWLKDAGAEIVDISLPHTKYALPAYYIIAPA
EASSNLARYDGVRYGLRDLPDGAGLQDMYAATREEGFGDEVKRRILIGTYVLSAGFYDAYYNQAQKIRTLVARDFEQAWA
ECDLILAPTTPNAAFGLGEMQADPLAMYLNDVFAVPASLAGLPAISVPGGLNSDGLPLGLQLVGKPFDEQGVLNAGLAIE
QRNGFTARPETWWA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=38.7878787878788, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI195972892, Length=454, Percent_Identity=25.1101321585903, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=446, Percent_Identity=39.0134529147982, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=519, Percent_Identity=22.7360308285164, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=24.223602484472, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=36.3636363636364, Blast_Score=243, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=463, Percent_Identity=27.4298056155508, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648113, Length=483, Percent_Identity=40.1656314699793, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=22.6452905811623, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=22.6452905811623, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=22.6452905811623, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=22.6452905811623, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=23.1827111984283, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550774, Length=525, Percent_Identity=22.4761904761905, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648435, Length=525, Percent_Identity=22.4761904761905, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=525, Percent_Identity=22.4761904761905, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=23.5177865612648, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52077; Mature: 51946

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELTELGVADIRDGVADGSFTAREVAEAFNAQVAAAKDLGAFIVETPEKALEAAAAVD
CCCCHHHCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCHHEEECHHHHHHHHHHHH
AARANGETLGSMAGVPIGMKDLFCTDGVQTTAASHILEGFVPQYESTVSANLWRAGAGML
HHHCCCHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
GKLNMDQFAMGSSNETSYFGPVKSPWRKAGSNTALTPGGSSGGSSSAVAARLCPAATGTD
ECCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCC
TGGSIRQPAAITGTTGIKPTYGRCSRWGVVAFASSLDQAGPIARSVRDCAIMLEAMAGFD
CCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
PKDATSLDMPVPQWEAGLSADLSGKKIGIPREYRMDGTPQDILDSWERGKKWLKDAGAEI
CCCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
VDISLPHTKYALPAYYIIAPAEASSNLARYDGVRYGLRDLPDGAGLQDMYAATREEGFGD
EEEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCH
EVKRRILIGTYVLSAGFYDAYYNQAQKIRTLVARDFEQAWAECDLILAPTTPNAAFGLGE
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
MQADPLAMYLNDVFAVPASLAGLPAISVPGGLNSDGLPLGLQLVGKPFDEQGVLNAGLAI
CCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEHHHHCCCCCCCCCCCCCEEE
EQRNGFTARPETWWA
ECCCCCCCCCCCCCC
>Mature Secondary Structure 
SSELTELGVADIRDGVADGSFTAREVAEAFNAQVAAAKDLGAFIVETPEKALEAAAAVD
CCCHHHCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCHHEEECHHHHHHHHHHHH
AARANGETLGSMAGVPIGMKDLFCTDGVQTTAASHILEGFVPQYESTVSANLWRAGAGML
HHHCCCHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
GKLNMDQFAMGSSNETSYFGPVKSPWRKAGSNTALTPGGSSGGSSSAVAARLCPAATGTD
ECCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCHHHHHHHCCCCCCCC
TGGSIRQPAAITGTTGIKPTYGRCSRWGVVAFASSLDQAGPIARSVRDCAIMLEAMAGFD
CCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
PKDATSLDMPVPQWEAGLSADLSGKKIGIPREYRMDGTPQDILDSWERGKKWLKDAGAEI
CCCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
VDISLPHTKYALPAYYIIAPAEASSNLARYDGVRYGLRDLPDGAGLQDMYAATREEGFGD
EEEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCH
EVKRRILIGTYVLSAGFYDAYYNQAQKIRTLVARDFEQAWAECDLILAPTTPNAAFGLGE
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
MQADPLAMYLNDVFAVPASLAGLPAISVPGGLNSDGLPLGLQLVGKPFDEQGVLNAGLAI
CCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEHHHHCCCCCCCCCCCCCEEE
EQRNGFTARPETWWA
ECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA