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The map label for this gene is gloA [H]

Identifier: 85373074

GI number: 85373074

Start: 287009

End: 287446

Strand: Direct

Name: gloA [H]

Synonym: ELI_01235

Alternate gene names: 85373074

Gene position: 287009-287446 (Clockwise)

Preceding gene: 85373072

Following gene: 85373076

Centisome position: 9.4

GC content: 61.19

Gene sequence:

>438_bases
ATGGTCAAATATCTGCATTCGATGATCCGGGTCGCCGACCCGGACGCGGCAGTCGATTTTTTCAAATTGATTGGCTTGGA
AGAGGTGCGCCGCTTCGATGTCGAAGCTGGGCGGTTCACGCTGATCTTCCTCGCCGCGCCCGGGCAGGAAGGCGTGGCCG
AGGTCGAACTGACCTACAACTGGCCGCCCGAAGACGGCAGCGAACCGGAGGACTATGACGGTGGCCGCAATTTCGGCCAT
CTCGCCTACCGGGTCGACAATATCTACGAGACCTGCCAGCGGCTGATGGATGCGGGTGTGACGATCAACCGTCCGCCGCG
CGACGGGCACATGGCCTTCGTCAAGTCACCCGACGGAATATCGGTCGAATTGCTGCAGGAAGGCAATCTGCCGCCGCAGG
AACCCTGGGCCAGCATGGAGAATACCGGGAGCTGGTGA

Upstream 100 bases:

>100_bases
GTTTCGGGTCTGGTCTCCTCGCTCATTCATTCCTCCATCCGTCTCACCCTGCGACGAGTTGTGCATTTGTGTCGCTGAAC
CGTTTCTGAAGGAAAGCTGC

Downstream 100 bases:

>100_bases
GCCGCTCGCGCTCCGTCAGGGGGTGACTGTCTGGGGCGGTCGCTCTTCGGGATGCGTCGTCGTCAGGCGCAGGATGGTGT
AGCCGAGGATCGCCGAGATC

Product: lactoylglutathione lyase, putative

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MVKYLHSMIRVADPDAAVDFFKLIGLEEVRRFDVEAGRFTLIFLAAPGQEGVAEVELTYNWPPEDGSEPEDYDGGRNFGH
LAYRVDNIYETCQRLMDAGVTINRPPRDGHMAFVKSPDGISVELLQEGNLPPQEPWASMENTGSW

Sequences:

>Translated_145_residues
MVKYLHSMIRVADPDAAVDFFKLIGLEEVRRFDVEAGRFTLIFLAAPGQEGVAEVELTYNWPPEDGSEPEDYDGGRNFGH
LAYRVDNIYETCQRLMDAGVTINRPPRDGHMAFVKSPDGISVELLQEGNLPPQEPWASMENTGSW
>Mature_145_residues
MVKYLHSMIRVADPDAAVDFFKLIGLEEVRRFDVEAGRFTLIFLAAPGQEGVAEVELTYNWPPEDGSEPEDYDGGRNFGH
LAYRVDNIYETCQRLMDAGVTINRPPRDGHMAFVKSPDGISVELLQEGNLPPQEPWASMENTGSW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=148, Percent_Identity=31.0810810810811, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=32.5757575757576, Blast_Score=77, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323639, Length=157, Percent_Identity=29.9363057324841, Blast_Score=68, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16288; Mature: 16288

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYLHSMIRVADPDAAVDFFKLIGLEEVRRFDVEAGRFTLIFLAAPGQEGVAEVELTYN
CHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPEDGSEPEDYDGGRNFGHLAYRVDNIYETCQRLMDAGVTINRPPRDGHMAFVKSPDGI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SVELLQEGNLPPQEPWASMENTGSW
EEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKYLHSMIRVADPDAAVDFFKLIGLEEVRRFDVEAGRFTLIFLAAPGQEGVAEVELTYN
CHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPEDGSEPEDYDGGRNFGHLAYRVDNIYETCQRLMDAGVTINRPPRDGHMAFVKSPDGI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SVELLQEGNLPPQEPWASMENTGSW
EEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]