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The map label for this gene is lcfB [H]

Identifier: 85373022

GI number: 85373022

Start: 230138

End: 231682

Strand: Direct

Name: lcfB [H]

Synonym: ELI_00975

Alternate gene names: 85373022

Gene position: 230138-231682 (Clockwise)

Preceding gene: 85373021

Following gene: 85373023

Centisome position: 7.54

GC content: 59.74

Gene sequence:

>1545_bases
ATGGAATTGCGCACCCAACTCCGCCGGGCGGCCGGCTATTTCGCCTGCAACGAAGCGTTGGTCCACGGCGAGCGCCGATT
CAGCTTTGCCGAAGCGTGGTCGCGGGGGGTGCGGCTTGCGAACGCGCTGACGAACGCGGGGCTGCGGCCGGGCGACAAGA
TCGCCACGCTGGAGAAGAACTCGATTGAAGCGGCTGACATTTTCCTCGCGGCAGCAATCGGCAATTTCGTTCGTGTACCG
CTCTATGCCCGCAATCGCCGAGAATCCCATGCCCATATGATGCGCAACACCGACTGCCGGTTAGCGCTCGTTGACAACGC
GCTCTTGCCCGAGGTCGCGGGACTCGAGACCGAATTGCCCGACTTGAAGCGACTGATCGTGCGCGACGGGACTTACGAGG
AATGGCTCGCCCGGGCATCTGATCAGGATCCGGACCCTACCATCTCGCCAGATGATTATTGCGTTATCCGTCACACAGGG
GGCACGACCGGTGCGCCGAAGGGCGTCGCCTATACCCATCGCACGTGGATGACTTGCTGCGCTGGCTTTCTGGAGGTGGG
ACCAAGACCTGATCCAGGCGCGACGGTATTGCATGTGGGGCCACTTTCGCACGCCTCCGGGTTCATGTTCTCTCCCTATT
GGGCGGTGGGTAGCCGCAACATTATGGTGGAGGCGTTTGACCCGGCAAGCTTCCTCGAGATACTGGAACGCGAAGAGGTC
GGCTATGCGTTCGTCGCTCCGACCATGCTCAACGCAGTTGTTCACCACGGCAATCCGCGTGGGCACGTTTTTCCGCGCTT
GAGATGTTTGCTCAGTGCTTCTGCGCCAGTTTCCGAAGCGACGTTGCGTAAGGCCCGCTCGATTTTCGGAGACGATGTGT
TGCACACCGCTTACGGACAGACCGAAATTCTGCCGGTGACTGCCCTAGGCCCAAAGGAATGGTTCGGCGAAACCGAAGGA
TCGACGCCGCTCCAATCGGTGGGTCGGGCGATGAGCTTTGTTGATCTCGAAATCCGTGATGCCGACGGTCGCGCTCTTGG
GCCTGACGAACCCGGAGAAATTGTCGCCCGGTTCGAAAACGGCCAGATGCAAGGCTTCTGGAACGATCCTGAGGAGACCG
CGCTACGCCTCGTCGACGGCTGGGTAAAGACTGGCGATATTGGTCGCATCGACGCAAACGGGTTCCTCTATCTGCTCGAT
CGCGCAAACGACCTGATCGTGTCGGGAGGATACAACATCTATCCGGCCGAAATCGAGAATGTGATCGCCGATCATCCTCA
AGTGATTGCCGCCGCCGTATTCGGTATCCCTCATGAAAAGTGGGGCGAAACGCCGCTTGCGCTGGTCGTGGTCGCGCCCG
GCACTGAATTGCCAGAGCAGGAAATAATCGATTTGGTTAGCGAACGGTTGGGTTCGTTCAAGAAGCCCGGCAAGGTCGTC
TTCACCACCGAACCTCTGCCGCTCAGCAACGTCGGCAAAGTGCTACGATCGAAACTCCGAGAGCCTTACTGGGCGGGGCG
GGCCAGCAGAGTTTCAGGCGCATGA

Upstream 100 bases:

>100_bases
ATGTTTGAAGTCTAAGCGGAGAAAATCCGATGGAGAGGATACATATGCGTGGAAACTGCGCAAACGGATGCAGCGCCGCC
CCGTGCAAGGGGCAGCGCTA

Downstream 100 bases:

>100_bases
GGGCAACTTCTCCTGACTCAAAAGTGGCAGACCTCGTGTCGCTCGACGCCATCACCGTTGAGAGGCGTGGCGCGGCTTTT
TGGGTGCACCTAAATCGTCC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MELRTQLRRAAGYFACNEALVHGERRFSFAEAWSRGVRLANALTNAGLRPGDKIATLEKNSIEAADIFLAAAIGNFVRVP
LYARNRRESHAHMMRNTDCRLALVDNALLPEVAGLETELPDLKRLIVRDGTYEEWLARASDQDPDPTISPDDYCVIRHTG
GTTGAPKGVAYTHRTWMTCCAGFLEVGPRPDPGATVLHVGPLSHASGFMFSPYWAVGSRNIMVEAFDPASFLEILEREEV
GYAFVAPTMLNAVVHHGNPRGHVFPRLRCLLSASAPVSEATLRKARSIFGDDVLHTAYGQTEILPVTALGPKEWFGETEG
STPLQSVGRAMSFVDLEIRDADGRALGPDEPGEIVARFENGQMQGFWNDPEETALRLVDGWVKTGDIGRIDANGFLYLLD
RANDLIVSGGYNIYPAEIENVIADHPQVIAAAVFGIPHEKWGETPLALVVVAPGTELPEQEIIDLVSERLGSFKKPGKVV
FTTEPLPLSNVGKVLRSKLREPYWAGRASRVSGA

Sequences:

>Translated_514_residues
MELRTQLRRAAGYFACNEALVHGERRFSFAEAWSRGVRLANALTNAGLRPGDKIATLEKNSIEAADIFLAAAIGNFVRVP
LYARNRRESHAHMMRNTDCRLALVDNALLPEVAGLETELPDLKRLIVRDGTYEEWLARASDQDPDPTISPDDYCVIRHTG
GTTGAPKGVAYTHRTWMTCCAGFLEVGPRPDPGATVLHVGPLSHASGFMFSPYWAVGSRNIMVEAFDPASFLEILEREEV
GYAFVAPTMLNAVVHHGNPRGHVFPRLRCLLSASAPVSEATLRKARSIFGDDVLHTAYGQTEILPVTALGPKEWFGETEG
STPLQSVGRAMSFVDLEIRDADGRALGPDEPGEIVARFENGQMQGFWNDPEETALRLVDGWVKTGDIGRIDANGFLYLLD
RANDLIVSGGYNIYPAEIENVIADHPQVIAAAVFGIPHEKWGETPLALVVVAPGTELPEQEIIDLVSERLGSFKKPGKVV
FTTEPLPLSNVGKVLRSKLREPYWAGRASRVSGA
>Mature_514_residues
MELRTQLRRAAGYFACNEALVHGERRFSFAEAWSRGVRLANALTNAGLRPGDKIATLEKNSIEAADIFLAAAIGNFVRVP
LYARNRRESHAHMMRNTDCRLALVDNALLPEVAGLETELPDLKRLIVRDGTYEEWLARASDQDPDPTISPDDYCVIRHTG
GTTGAPKGVAYTHRTWMTCCAGFLEVGPRPDPGATVLHVGPLSHASGFMFSPYWAVGSRNIMVEAFDPASFLEILEREEV
GYAFVAPTMLNAVVHHGNPRGHVFPRLRCLLSASAPVSEATLRKARSIFGDDVLHTAYGQTEILPVTALGPKEWFGETEG
STPLQSVGRAMSFVDLEIRDADGRALGPDEPGEIVARFENGQMQGFWNDPEETALRLVDGWVKTGDIGRIDANGFLYLLD
RANDLIVSGGYNIYPAEIENVIADHPQVIAAAVFGIPHEKWGETPLALVVVAPGTELPEQEIIDLVSERLGSFKKPGKVV
FTTEPLPLSNVGKVLRSKLREPYWAGRASRVSGA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=505, Percent_Identity=27.5247524752475, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=26.2857142857143, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI122937307, Length=504, Percent_Identity=25.9920634920635, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI38505220, Length=507, Percent_Identity=28.0078895463511, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI58082049, Length=515, Percent_Identity=25.4368932038835, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157311624, Length=515, Percent_Identity=25.0485436893204, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157311622, Length=515, Percent_Identity=25.0485436893204, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=25.9398496240602, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=25.9398496240602, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI115511026, Length=509, Percent_Identity=25.147347740668, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28416953, Length=523, Percent_Identity=23.9005736137667, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI13375727, Length=402, Percent_Identity=26.6169154228856, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8923896, Length=564, Percent_Identity=21.2765957446809, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI253970489, Length=395, Percent_Identity=23.2911392405063, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=364, Percent_Identity=29.9450549450549, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI221142682, Length=504, Percent_Identity=27.1825396825397, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693145, Length=498, Percent_Identity=25.7028112449799, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=25.9047619047619, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=26.3565891472868, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788595, Length=367, Percent_Identity=28.0653950953678, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786529, Length=531, Percent_Identity=25.0470809792844, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=25.1004016064257, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=26.0162601626016, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=520, Percent_Identity=25, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=520, Percent_Identity=25, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=520, Percent_Identity=25, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=442, Percent_Identity=25.7918552036199, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=26.8924302788845, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=506, Percent_Identity=25.6916996047431, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=388, Percent_Identity=26.8041237113402, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=385, Percent_Identity=29.0909090909091, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=519, Percent_Identity=24.0847784200385, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=406, Percent_Identity=26.6009852216749, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983001, Length=534, Percent_Identity=25.0936329588015, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=534, Percent_Identity=25.0936329588015, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17541856, Length=497, Percent_Identity=24.1448692152917, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=403, Percent_Identity=27.7915632754342, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=411, Percent_Identity=24.8175182481752, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=537, Percent_Identity=20.6703910614525, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859661, Length=365, Percent_Identity=31.5068493150685, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=27.3244781783681, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24581924, Length=541, Percent_Identity=25.8780036968577, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=26, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21356441, Length=492, Percent_Identity=25.8130081300813, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648253, Length=477, Percent_Identity=26.4150943396226, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648255, Length=477, Percent_Identity=26.2054507337526, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656500, Length=496, Percent_Identity=26.6129032258064, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161076582, Length=507, Percent_Identity=26.0355029585799, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=26.2626262626263, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21358303, Length=332, Percent_Identity=29.2168674698795, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281365686, Length=485, Percent_Identity=24.7422680412371, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356947, Length=497, Percent_Identity=24.9496981891348, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648260, Length=490, Percent_Identity=26.9387755102041, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI20130357, Length=358, Percent_Identity=27.0949720670391, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648257, Length=375, Percent_Identity=30.6666666666667, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI62472339, Length=554, Percent_Identity=24.7292418772563, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24667955, Length=539, Percent_Identity=24.6753246753247, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667959, Length=390, Percent_Identity=25.6410256410256, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21355225, Length=544, Percent_Identity=22.9779411764706, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221329755, Length=105, Percent_Identity=35.2380952380952, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56231; Mature: 56231

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRTQLRRAAGYFACNEALVHGERRFSFAEAWSRGVRLANALTNAGLRPGDKIATLEKN
CCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
SIEAADIFLAAAIGNFVRVPLYARNRRESHAHMMRNTDCRLALVDNALLPEVAGLETELP
CCHHHHHHHHHHHHHHEECCHHHCCCCHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCH
DLKRLIVRDGTYEEWLARASDQDPDPTISPDDYCVIRHTGGTTGAPKGVAYTHRTWMTCC
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHH
AGFLEVGPRPDPGATVLHVGPLSHASGFMFSPYWAVGSRNIMVEAFDPASFLEILEREEV
HHHHHCCCCCCCCCEEEEECCCCCCCCCEECCEEECCCCEEEEEECCCHHHHHHHHHHCC
GYAFVAPTMLNAVVHHGNPRGHVFPRLRCLLSASAPVSEATLRKARSIFGDDVLHTAYGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCC
TEILPVTALGPKEWFGETEGSTPLQSVGRAMSFVDLEIRDADGRALGPDEPGEIVARFEN
CEEEEEEECCCHHHCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEECC
GQMQGFWNDPEETALRLVDGWVKTGDIGRIDANGFLYLLDRANDLIVSGGYNIYPAEIEN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCEEEEECCCEECHHHHHH
VIADHPQVIAAAVFGIPHEKWGETPLALVVVAPGTELPEQEIIDLVSERLGSFKKPGKVV
HHHCCHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEE
FTTEPLPLSNVGKVLRSKLREPYWAGRASRVSGA
EEECCCCHHHHHHHHHHHHCCCHHCCCCCCCCCC
>Mature Secondary Structure
MELRTQLRRAAGYFACNEALVHGERRFSFAEAWSRGVRLANALTNAGLRPGDKIATLEKN
CCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
SIEAADIFLAAAIGNFVRVPLYARNRRESHAHMMRNTDCRLALVDNALLPEVAGLETELP
CCHHHHHHHHHHHHHHEECCHHHCCCCHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCH
DLKRLIVRDGTYEEWLARASDQDPDPTISPDDYCVIRHTGGTTGAPKGVAYTHRTWMTCC
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHH
AGFLEVGPRPDPGATVLHVGPLSHASGFMFSPYWAVGSRNIMVEAFDPASFLEILEREEV
HHHHHCCCCCCCCCEEEEECCCCCCCCCEECCEEECCCCEEEEEECCCHHHHHHHHHHCC
GYAFVAPTMLNAVVHHGNPRGHVFPRLRCLLSASAPVSEATLRKARSIFGDDVLHTAYGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCC
TEILPVTALGPKEWFGETEGSTPLQSVGRAMSFVDLEIRDADGRALGPDEPGEIVARFEN
CEEEEEEECCCHHHCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEECC
GQMQGFWNDPEETALRLVDGWVKTGDIGRIDANGFLYLLDRANDLIVSGGYNIYPAEIEN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCEEEEECCCEECHHHHHH
VIADHPQVIAAAVFGIPHEKWGETPLALVVVAPGTELPEQEIIDLVSERLGSFKKPGKVV
HHHCCHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEE
FTTEPLPLSNVGKVLRSKLREPYWAGRASRVSGA
EEECCCCHHHHHHHHHHHHCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]