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The map label for this gene is traD [H]

Identifier: 85372982

GI number: 85372982

Start: 186960

End: 189299

Strand: Direct

Name: traD [H]

Synonym: ELI_00775

Alternate gene names: 85372982

Gene position: 186960-189299 (Clockwise)

Preceding gene: 85372981

Following gene: 85372983

Centisome position: 6.13

GC content: 62.56

Gene sequence:

>2340_bases
ATGAAGCATAATCTCGTCAACTTCACCCGCGGCAGCCAGCTCCTTGGCCACTTCGGTTTCATGTTTGCGGCCGGTCTCAA
GGGGCCGCTGATCGTCACAATTCTGGTCGCCGTGGGCCTCCTCTACTGGGAAGTGAGCGGGGCGCTCGACGACCACCAGA
TCTACCTGCTGTGGATGCATGTCTACTCTTCGGCATATGCCTTCATGGAGTTCGATCCCGACAAGCTCGTGAACCTCAAG
CTGGCCGATGGCAGCATGGTCGCTTTCCCCATTTCGGTAGTCACGGACTACCCGCCAATGCGCGAGGCAGTTACGCTTTT
CCGGTCGACAGCTGTCGGCGCGCTCTGGCTTGCCGCACTGATCCTTGCCCCCCTTTTCCTGCTCTTTTGGTGGGTCGCCG
AGCGCTTCGGTGAGCGCTCGAAGCAGAAGAAGCATGTACGCGGCGCGGAGCTGGCAACCCTCTCCGAACTGAAGCGCGAG
ATCGAACGGCACAACCGCGATGCGCGTTCGCGCGAATATGGCGAAACTATGGGCTGGAAATGGCGACTTGCGGGCTCCAC
TTACCTTCGCGATGCAGGCTTCTATGCTCCCGCCCATCTCGCCCGTGTCAGCTGGCCCTGGCGGCTCGAGCAGAGCCACG
CGATGTTGGTCGGCACGACCGGGACGGGCAAGACGGTGGTGCTTACCGAGCTGGCGAACGAGATCCGGCAACGGGGCGAG
CGCGCGGTCATCTTCGACCTGACCGGGGCCTTCATCGAGACCTTCTACGAGCCCGAACGCGACATCATCCTGAACCCGCT
CGACGCGCGCTGTCCGGCATGGAGCGTGTTCAACGATTGCACGACCCGCGCCGAATTCCATGCGGCGGCGGAGTCCCTGG
TTCCTCATGATGGTGGCGGCGCAGAACAGTTCTGGATGCTCGCTGCGCGCACGCTGTTCGTCGAGACCTGCCTCAAGCTC
GCCGAGGCTGGAAGGGGCACCAATGCCGCGCTCGCGCACGAGTTGATGAATGCCGATCTTTCCGACCTGCACCAGCTGCT
CGAAGACACAATGGCGGGACCGATCAGCACGCCAAGCGCCGCACGCATGGCGGAATCGGTGCGCGCGGTGTTCAACGTCA
ACGCCAAGGCGCTGCAGATGCTTCCCGAAGAGGGCGTGCCCTTTTCGGTCCGCGAATGGGTAAACGGGGCGGCAAAACCC
GGCTCGATCCTGTTCCTCTCGGCGCGCTACATCGACATGAGCGTGCTATCGCAGCTGCTCACCTTGTGGCTCGATACATC
GATCAACACGCTGATGGCGGGCGAGCGTTCGCGGGACCTCAGACTGTGGTTCCTGATCGACGAATTGGGTGCGCTGCACC
GCCTGCCTTCGCTCGAGAAGGGCCTCCAGACCGCGCGCAATTTCGGGGGTGCGATCGTGACAGGCATCCATGCCTTTGCC
AAGCTCAAGGACGTTTACGGTGAGAACATGGCGATGACCCTGTCGTCGCTCGCCCGCACCAAGCTGATCCTCGGTACCGC
CGACCGCGAAACCGCCACCTGGTGTTCCGATGTCATCGGTCACCGCGAGGTTCGCGAGATGGAGGAAGGCTACAGCTACG
GCTACAACAACGCCCGCGATGCGGTCAGTCTTACGCCGCGGCGTCAGGTGGTCCCGCTGCTGCTTCCCGACGAGCTGATG
AGGCTCAAGAACCTCCACGGCTTCATCAAGTTTCCTGAAGGATTTCCCGCGGCGCCGGTCGTCTTGCAGCCTCGAAATTG
GCCCCGGGTAGCAGAAGGTTTCATCGCCCGAGAGATCCCCAAGATGCCAAAAAGGGCAGCGTCCGCAAGCGGCGATGCGG
GAACCATGACACAGCCAACCAGAGCTTCCGAAACGAGCGACAATGACGGTGATCCGCGCCGGATGATGGACAAGCACGAT
CAGTCCAATCGTCCCGCAAAGAGGCCCCGCAAGGACCAGCAAAAGGAACCAGGCCGAGATCGCCGATCCACAAGCGCCAA
GCAGGCGCTGCCGGAACAAACCAAAGCGCGAAGCCCTCGCGATGGAGGACAAAGGGGCGCGCTTCCTGCCCCACCTCGGC
CCGCCCAGTCCGAGCTTCCCTTGGCCTCCAAGACCGACGAGGATCGTGACAGGGAGCGGGAAACTGCACCGTCGAAGCGG
TCCGACGTTCCCGATCCTGCGACCCATCAGCGAGCGCAGGACGCCCGCGGCAAAGCCGACCAGAAGATGCAGGAGGAGCA
GCAGAAATCGCTCCTGGGCGGCGCTGCGAAGCAAGGGAAGGGTGCTGACCGCGATCACGACCACGACCATGATCTCGGCG
ACTACGATCCCGAAATGTGA

Upstream 100 bases:

>100_bases
CACCATCCGCATGCCGATCTGCGCGAGAAAACGGTCGCTGCCTACCAGCGTCGGTTGGAGCGCCTCGGGATCGTTGCACC
CCGGCCGCAGGGAGGCGACG

Downstream 100 bases:

>100_bases
CGCCGACCAGATGAGGGGGAGGGCTTACGAAAGGTGATCCTGCCCCATGCTTTCCGTCGCCAATGTCCGCTCGCCAACGG
CGGCGGCAAGCTACTTCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTILVAVGLLYWEVSGALDDHQIYLLWMHVYSSAYAFMEFDPDKLVNLK
LADGSMVAFPISVVTDYPPMREAVTLFRSTAVGALWLAALILAPLFLLFWWVAERFGERSKQKKHVRGAELATLSELKRE
IERHNRDARSREYGETMGWKWRLAGSTYLRDAGFYAPAHLARVSWPWRLEQSHAMLVGTTGTGKTVVLTELANEIRQRGE
RAVIFDLTGAFIETFYEPERDIILNPLDARCPAWSVFNDCTTRAEFHAAAESLVPHDGGGAEQFWMLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHQLLEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEEGVPFSVREWVNGAAKP
GSILFLSARYIDMSVLSQLLTLWLDTSINTLMAGERSRDLRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDVIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
RLKNLHGFIKFPEGFPAAPVVLQPRNWPRVAEGFIAREIPKMPKRAASASGDAGTMTQPTRASETSDNDGDPRRMMDKHD
QSNRPAKRPRKDQQKEPGRDRRSTSAKQALPEQTKARSPRDGGQRGALPAPPRPAQSELPLASKTDEDRDRERETAPSKR
SDVPDPATHQRAQDARGKADQKMQEEQQKSLLGGAAKQGKGADRDHDHDHDLGDYDPEM

Sequences:

>Translated_779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTILVAVGLLYWEVSGALDDHQIYLLWMHVYSSAYAFMEFDPDKLVNLK
LADGSMVAFPISVVTDYPPMREAVTLFRSTAVGALWLAALILAPLFLLFWWVAERFGERSKQKKHVRGAELATLSELKRE
IERHNRDARSREYGETMGWKWRLAGSTYLRDAGFYAPAHLARVSWPWRLEQSHAMLVGTTGTGKTVVLTELANEIRQRGE
RAVIFDLTGAFIETFYEPERDIILNPLDARCPAWSVFNDCTTRAEFHAAAESLVPHDGGGAEQFWMLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHQLLEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEEGVPFSVREWVNGAAKP
GSILFLSARYIDMSVLSQLLTLWLDTSINTLMAGERSRDLRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDVIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
RLKNLHGFIKFPEGFPAAPVVLQPRNWPRVAEGFIAREIPKMPKRAASASGDAGTMTQPTRASETSDNDGDPRRMMDKHD
QSNRPAKRPRKDQQKEPGRDRRSTSAKQALPEQTKARSPRDGGQRGALPAPPRPAQSELPLASKTDEDRDRERETAPSKR
SDVPDPATHQRAQDARGKADQKMQEEQQKSLLGGAAKQGKGADRDHDHDHDLGDYDPEM
>Mature_779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTILVAVGLLYWEVSGALDDHQIYLLWMHVYSSAYAFMEFDPDKLVNLK
LADGSMVAFPISVVTDYPPMREAVTLFRSTAVGALWLAALILAPLFLLFWWVAERFGERSKQKKHVRGAELATLSELKRE
IERHNRDARSREYGETMGWKWRLAGSTYLRDAGFYAPAHLARVSWPWRLEQSHAMLVGTTGTGKTVVLTELANEIRQRGE
RAVIFDLTGAFIETFYEPERDIILNPLDARCPAWSVFNDCTTRAEFHAAAESLVPHDGGGAEQFWMLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHQLLEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEEGVPFSVREWVNGAAKP
GSILFLSARYIDMSVLSQLLTLWLDTSINTLMAGERSRDLRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDVIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
RLKNLHGFIKFPEGFPAAPVVLQPRNWPRVAEGFIAREIPKMPKRAASASGDAGTMTQPTRASETSDNDGDPRRMMDKHD
QSNRPAKRPRKDQQKEPGRDRRSTSAKQALPEQTKARSPRDGGQRGALPAPPRPAQSELPLASKTDEDRDRERETAPSKR
SDVPDPATHQRAQDARGKADQKMQEEQQKSLLGGAAKQGKGADRDHDHDHDLGDYDPEM

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 86767; Mature: 86767

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTILVAVGLLYWEVSGALDDHQIYLLWMH
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
VYSSAYAFMEFDPDKLVNLKLADGSMVAFPISVVTDYPPMREAVTLFRSTAVGALWLAAL
HHHHHHEEEEECCCCEEEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILAPLFLLFWWVAERFGERSKQKKHVRGAELATLSELKREIERHNRDARSREYGETMGWK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
WRLAGSTYLRDAGFYAPAHLARVSWPWRLEQSHAMLVGTTGTGKTVVLTELANEIRQRGE
EEECCCHHHHCCCCCCCHHHHHCCCCEEECCCCEEEEEECCCCCEEEHHHHHHHHHHCCC
RAVIFDLTGAFIETFYEPERDIILNPLDARCPAWSVFNDCTTRAEFHAAAESLVPHDGGG
EEEEEECHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEQFWMLAARTLFVETCLKLAEAGRGTNAALAHELMNADLSDLHQLLEDTMAGPISTPSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ARMAESVRAVFNVNAKALQMLPEEGVPFSVREWVNGAAKPGSILFLSARYIDMSVLSQLL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHH
TLWLDTSINTLMAGERSRDLRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
HHHHHCCHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDVIGHREVREMEEGYSYGYNNARD
HHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVSLTPRRQVVPLLLPDELMRLKNLHGFIKFPEGFPAAPVVLQPRNWPRVAEGFIAREIP
CEECCCCCCEECEECCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHH
KMPKRAASASGDAGTMTQPTRASETSDNDGDPRRMMDKHDQSNRPAKRPRKDQQKEPGRD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
RRSTSAKQALPEQTKARSPRDGGQRGALPAPPRPAQSELPLASKTDEDRDRERETAPSKR
HHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
SDVPDPATHQRAQDARGKADQKMQEEQQKSLLGGAAKQGKGADRDHDHDHDLGDYDPEM
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTILVAVGLLYWEVSGALDDHQIYLLWMH
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
VYSSAYAFMEFDPDKLVNLKLADGSMVAFPISVVTDYPPMREAVTLFRSTAVGALWLAAL
HHHHHHEEEEECCCCEEEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILAPLFLLFWWVAERFGERSKQKKHVRGAELATLSELKREIERHNRDARSREYGETMGWK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
WRLAGSTYLRDAGFYAPAHLARVSWPWRLEQSHAMLVGTTGTGKTVVLTELANEIRQRGE
EEECCCHHHHCCCCCCCHHHHHCCCCEEECCCCEEEEEECCCCCEEEHHHHHHHHHHCCC
RAVIFDLTGAFIETFYEPERDIILNPLDARCPAWSVFNDCTTRAEFHAAAESLVPHDGGG
EEEEEECHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEQFWMLAARTLFVETCLKLAEAGRGTNAALAHELMNADLSDLHQLLEDTMAGPISTPSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ARMAESVRAVFNVNAKALQMLPEEGVPFSVREWVNGAAKPGSILFLSARYIDMSVLSQLL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHH
TLWLDTSINTLMAGERSRDLRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
HHHHHCCHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDVIGHREVREMEEGYSYGYNNARD
HHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVSLTPRRQVVPLLLPDELMRLKNLHGFIKFPEGFPAAPVVLQPRNWPRVAEGFIAREIP
CEECCCCCCEECEECCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHH
KMPKRAASASGDAGTMTQPTRASETSDNDGDPRRMMDKHDQSNRPAKRPRKDQQKEPGRD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
RRSTSAKQALPEQTKARSPRDGGQRGALPAPPRPAQSELPLASKTDEDRDRERETAPSKR
HHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
SDVPDPATHQRAQDARGKADQKMQEEQQKSLLGGAAKQGKGADRDHDHDHDLGDYDPEM
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]