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The map label for this gene is cyoE [H]

Identifier: 84625568

GI number: 84625568

Start: 4411994

End: 4412890

Strand: Reverse

Name: cyoE [H]

Synonym: XOO_3911

Alternate gene names: 84625568

Gene position: 4412890-4411994 (Counterclockwise)

Preceding gene: 84625569

Following gene: 84625565

Centisome position: 89.33

GC content: 62.88

Gene sequence:

>897_bases
ATGGCTGTCAGGGCACGCGATTATTGGGATCTGACCAAACCCAAGGTGGTGGCACTGATCGTGTTCACCGCGTTGGTGGG
CATGTTCCTGGCCATTCCCGACATGCCGACCTGGCTGCAGGTACGCACCGGCGCGTTGGGCTTTCTGGGCATCTGGCTGG
CGGCTTCAGCGGCGGCAGCGATCAACCAACTGCTCGACGCCAAGATCGACGCACAGATGGCGCGCACGTCATGGCGTCCG
CTGGTGGTGGGCAAGGTGCAGCCATGGCAGGTGCTGGTGTTCGCCGGCGCGCTGATCGTGATCTCCATGACCATCCTGGT
GGTGTGGGTGAATGTCATCACCGCGGTGCTGACCTTCGCCTCGCTGATCGGTTATGCGGTGATCTACACCGTGTACCTGA
AACGCGCGACCTCGCAAAACATCGTGATCGGGGGCTTGGCCGGCGCCACGCCGCCGATGCTGGGCTGGGCCGCGGTGACC
GGGCTGCCGACCAGCGCCGACTGGATCAACGCCTCGCTGCTGGTGCTGATCATCTTCATCTGGACGCCGCCGCATTTTTG
GGCCCTGGCGATCTTTCGGCGCGCCGATTACGCCAAGGCGGCGATCCCGATGTTGCCGGTGACCCACGGCGTGCCGCATA
CGCGCAAGCAGATCCTGGTGTATACCGTGTTGTTGGCCATCGTGACCTTGCTGCCGGTGACGGTGGGCATGAGCGGGGTG
TTCTACCTGGGCGGTGCGGTCGTGCTCAACGCGGTGTTCCTCTGGTATGCGTGGCGCATGCTGAACCCGCCGGACGAACT
GTTTTCGATGAAGATGTTCGGCTATTCCATCGTGTATCTGATGGCGCTGTTCGCCTTTCTGATGGTCGACCATCTGCTGC
TGCCGTGGGTGCGCTAG

Upstream 100 bases:

>100_bases
CGCTGCCGCTGGAGGTCTCGGTCATGCATAACGGCGGCGCGGTGGCGTTGCTATTCGTGCTGGTCTCGCTGCAGGCGCGG
CTGCGCGCGCCGGAGTGAGC

Downstream 100 bases:

>100_bases
TCGCTGCGGTGGCGCGCGTGGCAACGTTGCAGGTCTGTCGTAAGAACGTCTCGGCAACAAGCGCATTCTGTGCAATGCGC
GCTGTCGCACGTGCGCAGTG

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAVRARDYWDLTKPKVVALIVFTALVGMFLAIPDMPTWLQVRTGALGFLGIWLAASAAAAINQLLDAKIDAQMARTSWRP
LVVGKVQPWQVLVFAGALIVISMTILVVWVNVITAVLTFASLIGYAVIYTVYLKRATSQNIVIGGLAGATPPMLGWAAVT
GLPTSADWINASLLVLIIFIWTPPHFWALAIFRRADYAKAAIPMLPVTHGVPHTRKQILVYTVLLAIVTLLPVTVGMSGV
FYLGGAVVLNAVFLWYAWRMLNPPDELFSMKMFGYSIVYLMALFAFLMVDHLLLPWVR

Sequences:

>Translated_298_residues
MAVRARDYWDLTKPKVVALIVFTALVGMFLAIPDMPTWLQVRTGALGFLGIWLAASAAAAINQLLDAKIDAQMARTSWRP
LVVGKVQPWQVLVFAGALIVISMTILVVWVNVITAVLTFASLIGYAVIYTVYLKRATSQNIVIGGLAGATPPMLGWAAVT
GLPTSADWINASLLVLIIFIWTPPHFWALAIFRRADYAKAAIPMLPVTHGVPHTRKQILVYTVLLAIVTLLPVTVGMSGV
FYLGGAVVLNAVFLWYAWRMLNPPDELFSMKMFGYSIVYLMALFAFLMVDHLLLPWVR
>Mature_297_residues
AVRARDYWDLTKPKVVALIVFTALVGMFLAIPDMPTWLQVRTGALGFLGIWLAASAAAAINQLLDAKIDAQMARTSWRPL
VVGKVQPWQVLVFAGALIVISMTILVVWVNVITAVLTFASLIGYAVIYTVYLKRATSQNIVIGGLAGATPPMLGWAAVTG
LPTSADWINASLLVLIIFIWTPPHFWALAIFRRADYAKAAIPMLPVTHGVPHTRKQILVYTVLLAIVTLLPVTVGMSGVF
YLGGAVVLNAVFLWYAWRMLNPPDELFSMKMFGYSIVYLMALFAFLMVDHLLLPWVR

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group [H]

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI17921982, Length=201, Percent_Identity=38.3084577114428, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1786631, Length=295, Percent_Identity=31.5254237288136, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71998235, Length=272, Percent_Identity=30.8823529411765, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325085, Length=197, Percent_Identity=33.502538071066, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921064, Length=285, Percent_Identity=31.2280701754386, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006369
- InterPro:   IPR000537 [H]

Pfam domain/function: PF01040 UbiA [H]

EC number: 2.5.1.- [C]

Molecular weight: Translated: 32815; Mature: 32684

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRARDYWDLTKPKVVALIVFTALVGMFLAIPDMPTWLQVRTGALGFLGIWLAASAAAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
INQLLDAKIDAQMARTSWRPLVVGKVQPWQVLVFAGALIVISMTILVVWVNVITAVLTFA
HHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIGYAVIYTVYLKRATSQNIVIGGLAGATPPMLGWAAVTGLPTSADWINASLLVLIIFI
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
WTPPHFWALAIFRRADYAKAAIPMLPVTHGVPHTRKQILVYTVLLAIVTLLPVTVGMSGV
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FYLGGAVVLNAVFLWYAWRMLNPPDELFSMKMFGYSIVYLMALFAFLMVDHLLLPWVR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVRARDYWDLTKPKVVALIVFTALVGMFLAIPDMPTWLQVRTGALGFLGIWLAASAAAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
INQLLDAKIDAQMARTSWRPLVVGKVQPWQVLVFAGALIVISMTILVVWVNVITAVLTFA
HHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIGYAVIYTVYLKRATSQNIVIGGLAGATPPMLGWAAVTGLPTSADWINASLLVLIIFI
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
WTPPHFWALAIFRRADYAKAAIPMLPVTHGVPHTRKQILVYTVLLAIVTLLPVTVGMSGV
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FYLGGAVVLNAVFLWYAWRMLNPPDELFSMKMFGYSIVYLMALFAFLMVDHLLLPWVR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]