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The map label for this gene is insK [C]

Identifier: 84625478

GI number: 84625478

Start: 4326453

End: 4327268

Strand: Reverse

Name: insK [C]

Synonym: XOO_3821

Alternate gene names: 84625478

Gene position: 4327268-4326453 (Counterclockwise)

Preceding gene: 84625479

Following gene: 84625477

Centisome position: 87.59

GC content: 63.6

Gene sequence:

>816_bases
GTGCGCGAGTGGATCGACGGTGGCGCCAGCGAGCGCTGCGCCCTGGCAGCGATCGGCATGAGCGCCAGTGCGCTGCGCTA
TCGCCCGCGCGAGGACCGCAACGTTGAGTTGCGCGAGCGCATCCTTGCGTTGGCGCATCGCCATCGCCGCTATGGCGTGG
GGATGATCTCTCTCAAGCTGCGGCAGGAAGGGCGCCTCGTGAACTATAAGCGGGTGGAGCGGCTGTATTGCGAGCAGCAG
CTGCAGGTCCGCCGCCGCACGCGTAAAAAGGTGCCGGTAGGCGAGCGTGCGCCGTTGCTGCGGCCCACCAAGGCCAACCA
GGTGTGGTCGATGGACGTCGTGTTCGACCGCACCGCCGAAGGCAGGGCAATCAAATGCCTGGTGATCGTGGACGACGCAA
CCCACGAAGCGGTCGCCATCGACGTGGAGCGTGCGATCTCGGGACACGGCGTTGTGCGCGTGCTGGATCGGTTGGCACAC
AGTCGTGGCCTGCCGAAGATGATCCGCACGGACAATGGCAAGGAGTTCTGTGGCAAGGCCATGGTCGCCTGGGCGCATGC
CAATCGTGTGCAGCTACGCCAGATCCAGCCTGGCAAGCCGAACCAGAATGCCTATGTCGAATCCTTCAACGGCCGGCTAC
GCGACGAATGCCTCAACGAACATGGGTTCCCAACGCTGCTGCATGCGCGCACCGAGATCGAACGCTGGCGCCGCGAATAC
AACGAACACCGCCCCAAAAAAACAATCGGCGGAATGACGCCGGCGGCCTATGCCCAGCAGTTGGCCAATAGCGATATCAT
CAACCCCGGACTCTAA

Upstream 100 bases:

>100_bases
CGCGACTGAAGAAGTTGCTGGCCGAGCAGGTGTTCCAGAACGACCTGATCAAGGATGCGCTGCAAAAACAATGGTGAGCG
CACCGGCGCGTCGTGCGCTG

Downstream 100 bases:

>100_bases
ACCCGACTGCTACTCAGGATGGGGGGACGTCGCGGCCACATCGCGGGCAAAAGCGTATCGCTCGGCCAGGCAGATGTGAT
GCCTTCGGTGATATGCACCT

Product: ISXoo3 transposase orfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPVGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATHEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHGFPTLLHARTEIERWRREY
NEHRPKKTIGGMTPAAYAQQLANSDIINPGL

Sequences:

>Translated_271_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPVGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATHEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHGFPTLLHARTEIERWRREY
NEHRPKKTIGGMTPAAYAQQLANSDIINPGL
>Mature_271_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPVGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATHEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHGFPTLLHARTEIERWRREY
NEHRPKKTIGGMTPAAYAQQLANSDIINPGL

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=244, Percent_Identity=23.7704918032787, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31041; Mature: 31041

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPVGERAPLLRPTKANQVWSMDVVFDRTAE
CCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GRAIKCLVIVDDATHEAVAIDVERAISGHGVVRVLDRLAHSRGLPKMIRTDNGKEFCGKA
CCEEEEEEEEECCCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHGFPTLLHARTEIERWRREY
HHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NEHRPKKTIGGMTPAAYAQQLANSDIINPGL
HHCCCHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPVGERAPLLRPTKANQVWSMDVVFDRTAE
CCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GRAIKCLVIVDDATHEAVAIDVERAISGHGVVRVLDRLAHSRGLPKMIRTDNGKEFCGKA
CCEEEEEEEEECCCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHGFPTLLHARTEIERWRREY
HHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NEHRPKKTIGGMTPAAYAQQLANSDIINPGL
HHCCCHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]