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The map label for this gene is glyA

Identifier: 84625296

GI number: 84625296

Start: 4114283

End: 4115536

Strand: Reverse

Name: glyA

Synonym: XOO_3639

Alternate gene names: 84625296

Gene position: 4115536-4114283 (Counterclockwise)

Preceding gene: 84625297

Following gene: 84625295

Centisome position: 83.31

GC content: 63.4

Gene sequence:

>1254_bases
ATGTTCTCGCGCGATGTCCGACTGGAAACCTACGACCCCGAACTGGCCAAGGCCATCGCTGCTGAAGCCGGCCGCCAGGA
GGATCACGTCGAGCTGATCGCCAGCGAGAACTACTGCAGCCAGCTGGTGATGGAAGCCCAGGGCAGCCAGCTGACCAACA
AGTACGCCGAAGGCTACCCTGGCAAGCGCTACTACGGCGGCTGCGCGTTCGTCGACATTGCCGAACAGCTAGCGATCGAC
CGCATCAAGCAGGTCTTCGATGCCGATTATGCAAATGTGCAGCCGCACAGCGGCTCGCAGGCCAACCAGGCGGTGTATCT
GGCGCTGCTGCAGCCGGGCGACACCATTTTGGGCATGAGCCTGGCGCATGGCGGCCACCTGACCCACGGCGCCAAGGCCA
ACGTCTCCGGCAAGCTGTTCAACGCGGTGCAGTACGGCGTCAACGAGCAGGGCCTGATCGATTACGACGAAGTGCAGCGT
CTTGCGACCGAGCACACGCCGAAGATGGTGGTGGCCGGTTTCTCGGCGTATTCGCAGAAGATCGACTGGGCCCGCTTCCG
TGCCATTGCCGACAGCGTTGGCGCCTATCTGTTCGTGGACATGGCCCACATCGCTGGCCTCGTGGCCGCCGGGGTGTATC
CCAGCCCGATGGAACATGCGCACGTGGTGACGTCGACGACGCACAAGACGCTGCGCGGTCCGCGTGGCGGCATCATCGTC
GCCAAGGGCGCCAGCGAAGAATTGCAGAAGAAGCTGCAGTCGATCGTGTTCCCCGGCATTCAGGGTGGCCCGCTGATGCA
CGTGATCGCCGCCAAGGCGGTTGCGTTCAAGGAAGCACTGGAGCCGGCGTTCAAGACCTACCAGCAACAGGTGGTCAAGA
ATGCTCAGGCGATGGCCAACACGCTGATCGCACGCGGGTACAAGATCGTCTCCGGCGGCACCGAAAACCATCTGATGCTG
GTCGACATGATCGGCCGCGACGTCTCCGGCAAGGATGCCGAAGCCGCGCTGGGCAAGGCACACATCACCGTCAACAAGAA
CGCGGTGCCCAACGACCCGCGCTCGCCGTTCGTTACCTCGGGCCTGCGCTTGGGTACCCCGGCAATCACCACGCGCGGTT
ACAAGGAACCGGACAGCATCGATCTGGCCAACTGGATCGCCGATGTGCTCGACGCCCCGACCGACGAAGCGGTGCTGGCG
AAAGTGCGCGATGCAGTGACCGCGCAGTGCAAGCGTTATCCGGTGTACGGCTGA

Upstream 100 bases:

>100_bases
CTGCTGTCGGACCCGGCCCGCGTGCAGGGCATATCACCTCAAAGCGCGCAACACGCTTTAAACTACGCGCCTGCCATCTG
TCCATTCCTCTGGAGACCTG

Downstream 100 bases:

>100_bases
GCGTCGCAACGTCATGGCGTTGATCGCACGCAGGCTGGGCGCAGCGTCGAAGTACGACCACCTTGCCTGTGTGCGTGCCG
CCGGTTTGCGCTGCGAAGTG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSQLVMEAQGSQLTNKYAEGYPGKRYYGGCAFVDIAEQLAID
RIKQVFDADYANVQPHSGSQANQAVYLALLQPGDTILGMSLAHGGHLTHGAKANVSGKLFNAVQYGVNEQGLIDYDEVQR
LATEHTPKMVVAGFSAYSQKIDWARFRAIADSVGAYLFVDMAHIAGLVAAGVYPSPMEHAHVVTSTTHKTLRGPRGGIIV
AKGASEELQKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLML
VDMIGRDVSGKDAEAALGKAHITVNKNAVPNDPRSPFVTSGLRLGTPAITTRGYKEPDSIDLANWIADVLDAPTDEAVLA
KVRDAVTAQCKRYPVYG

Sequences:

>Translated_417_residues
MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSQLVMEAQGSQLTNKYAEGYPGKRYYGGCAFVDIAEQLAID
RIKQVFDADYANVQPHSGSQANQAVYLALLQPGDTILGMSLAHGGHLTHGAKANVSGKLFNAVQYGVNEQGLIDYDEVQR
LATEHTPKMVVAGFSAYSQKIDWARFRAIADSVGAYLFVDMAHIAGLVAAGVYPSPMEHAHVVTSTTHKTLRGPRGGIIV
AKGASEELQKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLML
VDMIGRDVSGKDAEAALGKAHITVNKNAVPNDPRSPFVTSGLRLGTPAITTRGYKEPDSIDLANWIADVLDAPTDEAVLA
KVRDAVTAQCKRYPVYG
>Mature_417_residues
MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSQLVMEAQGSQLTNKYAEGYPGKRYYGGCAFVDIAEQLAID
RIKQVFDADYANVQPHSGSQANQAVYLALLQPGDTILGMSLAHGGHLTHGAKANVSGKLFNAVQYGVNEQGLIDYDEVQR
LATEHTPKMVVAGFSAYSQKIDWARFRAIADSVGAYLFVDMAHIAGLVAAGVYPSPMEHAHVVTSTTHKTLRGPRGGIIV
AKGASEELQKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLML
VDMIGRDVSGKDAEAALGKAHITVNKNAVPNDPRSPFVTSGLRLGTPAITTRGYKEPDSIDLANWIADVLDAPTDEAVLA
KVRDAVTAQCKRYPVYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=401, Percent_Identity=48.1296758104738, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862352, Length=401, Percent_Identity=48.1296758104738, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=401, Percent_Identity=48.1296758104738, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=401, Percent_Identity=48.1296758104738, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=400, Percent_Identity=47, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=48.3790523690773, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=46.7866323907455, Blast_Score=303, Evalue=1e-82,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=68.5096153846154, Blast_Score=594, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=47, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=47, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=397, Percent_Identity=47.3551637279597, Blast_Score=352, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=45.3400503778337, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640005, Length=410, Percent_Identity=47.8048780487805, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=410, Percent_Identity=47.8048780487805, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_XANOM (Q2NZ83)

Other databases:

- EMBL:   AP008229
- RefSeq:   YP_452668.1
- ProteinModelPortal:   Q2NZ83
- SMR:   Q2NZ83
- STRING:   Q2NZ83
- GeneID:   3857745
- GenomeReviews:   AP008229_GR
- KEGG:   xom:XOO_3639
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GGLIMTK
- PhylomeDB:   Q2NZ83
- ProtClustDB:   PRK00011
- BioCyc:   XORY342109:XOO3639-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44913; Mature: 44913

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSQLVMEAQGSQLTNKYAEGYP
CCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHCCCC
GKRYYGGCAFVDIAEQLAIDRIKQVFDADYANVQPHSGSQANQAVYLALLQPGDTILGMS
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGAKANVSGKLFNAVQYGVNEQGLIDYDEVQRLATEHTPKMVVAGFSAYSQK
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
IDWARFRAIADSVGAYLFVDMAHIAGLVAAGVYPSPMEHAHVVTSTTHKTLRGPRGGIIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHCCCCCCEEE
AKGASEELQKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKTYQQQVVKNAQAMAN
ECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNAVPNDPRSPFVTS
HHHHCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHCEEEEEECCCCCCCCCCCCHHHC
GLRLGTPAITTRGYKEPDSIDLANWIADVLDAPTDEAVLAKVRDAVTAQCKRYPVYG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSQLVMEAQGSQLTNKYAEGYP
CCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHCCCC
GKRYYGGCAFVDIAEQLAIDRIKQVFDADYANVQPHSGSQANQAVYLALLQPGDTILGMS
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGAKANVSGKLFNAVQYGVNEQGLIDYDEVQRLATEHTPKMVVAGFSAYSQK
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
IDWARFRAIADSVGAYLFVDMAHIAGLVAAGVYPSPMEHAHVVTSTTHKTLRGPRGGIIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHCCCCCCEEE
AKGASEELQKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKTYQQQVVKNAQAMAN
ECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNAVPNDPRSPFVTS
HHHHCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHCEEEEEECCCCCCCCCCCCHHHC
GLRLGTPAITTRGYKEPDSIDLANWIADVLDAPTDEAVLAKVRDAVTAQCKRYPVYG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA