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The map label for this gene is def1 [H]

Identifier: 84622632

GI number: 84622632

Start: 1064862

End: 1065377

Strand: Direct

Name: def1 [H]

Synonym: XOO_0975

Alternate gene names: 84622632

Gene position: 1064862-1065377 (Clockwise)

Preceding gene: 84622631

Following gene: 84622633

Centisome position: 21.55

GC content: 59.88

Gene sequence:

>516_bases
ATGATTCGCGACATTATCCGCATGGGCGACAAGCGCTTGCTGCGTGTGGCGCCACAGGTCACCAACCTGGGCAGCGCCGA
GTTGCACGCGCTGGTGTCCGACATGTTCGAAACCATGGGCGCTGCGCATGGCGTCGGTTTGGCAGCGCCGCAGATCGCGG
TGGATCTGCAATTGATGGTGTTCGGTTTCGAAGCCAGCGAGCGCTATCCCGAAGCACCCGCAGTACCGCTCACGGCTCTG
GCCAACGCACAGATCGAACCGCTATCGGACGAGATGGAAAACGGCTGGGAAGGCTGCCTGTCGATTCCCGGTCTGCGTGC
AGTGATTCCACGTTATCGCTACATTCGTTACCGCGGCTTTGCGCCCGACGGCAGCCCGATCGAGCGCGAGGCCGAAGGCT
TCCATGCACGCGTGGTGCAACACGAATACGATCACTTGGTCGGTCGTCTGTATCCCTCGCGCATCGAAAACTTTGACACC
TTCGGTTTCGACGACGTCTTGTCTTACGATCTGTAG

Upstream 100 bases:

>100_bases
ATTGCAGTTCCTGCGCGCTGTTCACTCCTGGCTCAAGATTGCTCGCTTACGTTCTGGCAGCTACTCCAAACTCCACGTCA
CGATCTTCAGGAGCACACCC

Downstream 100 bases:

>100_bases
CATGCCGTTCACGCTTTCCATAGCAGCCTAGCTGCAATGGAAAGCGGCCGTCCGTGCAAGATTGCGCCAGGCGGGCGCGG
CCGGTTCTCGGAATCGGCAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MIRDIIRMGDKRLLRVAPQVTNLGSAELHALVSDMFETMGAAHGVGLAAPQIAVDLQLMVFGFEASERYPEAPAVPLTAL
ANAQIEPLSDEMENGWEGCLSIPGLRAVIPRYRYIRYRGFAPDGSPIEREAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFDDVLSYDL

Sequences:

>Translated_171_residues
MIRDIIRMGDKRLLRVAPQVTNLGSAELHALVSDMFETMGAAHGVGLAAPQIAVDLQLMVFGFEASERYPEAPAVPLTAL
ANAQIEPLSDEMENGWEGCLSIPGLRAVIPRYRYIRYRGFAPDGSPIEREAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFDDVLSYDL
>Mature_171_residues
MIRDIIRMGDKRLLRVAPQVTNLGSAELHALVSDMFETMGAAHGVGLAAPQIAVDLQLMVFGFEASERYPEAPAVPLTAL
ANAQIEPLSDEMENGWEGCLSIPGLRAVIPRYRYIRYRGFAPDGSPIEREAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFDDVLSYDL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=173, Percent_Identity=31.2138728323699, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=41.2162162162162, Blast_Score=99, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24645726, Length=167, Percent_Identity=31.1377245508982, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19078; Mature: 19078

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDIIRMGDKRLLRVAPQVTNLGSAELHALVSDMFETMGAAHGVGLAAPQIAVDLQLMV
CCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFEASERYPEAPAVPLTALANAQIEPLSDEMENGWEGCLSIPGLRAVIPRYRYIRYRGF
EECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECC
APDGSPIEREAEGFHARVVQHEYDHLVGRLYPSRIENFDTFGFDDVLSYDL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIRDIIRMGDKRLLRVAPQVTNLGSAELHALVSDMFETMGAAHGVGLAAPQIAVDLQLMV
CCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFEASERYPEAPAVPLTALANAQIEPLSDEMENGWEGCLSIPGLRAVIPRYRYIRYRGF
EECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECC
APDGSPIEREAEGFHARVVQHEYDHLVGRLYPSRIENFDTFGFDDVLSYDL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]