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The map label for this gene is rnhA [H]

Identifier: 84622597

GI number: 84622597

Start: 1028062

End: 1028514

Strand: Direct

Name: rnhA [H]

Synonym: XOO_0940

Alternate gene names: 84622597

Gene position: 1028062-1028514 (Clockwise)

Preceding gene: 84622596

Following gene: 84622598

Centisome position: 20.81

GC content: 64.46

Gene sequence:

>453_bases
ATGAAATCAATTGAAGTGCATACCGATGGCTCCTGCCTCGGCAATCCCGGGCCGGGTGGCTGGGCGGCCCTGTTGCGTTA
CAACGGGCGCGAAAAGGAACTGGCCGGCGGCGAAGCCGTGTCCACCAATAACCGCATGGAGTTGATGGCGGCGATCATGG
CGCTGGAAACGCTGACCGAGCCGTGCGAGATCGTGTTGCATACCGACTCGCAATACGTGCGCCAGGGCATCACCGAGTGG
ATGCCGGGCTGGGTGCGGCGCAACTGGAAAACCGCCGGCGGCGATCCGGTCAAGAATCGCGAGTTGTGGGAGCGGCTGCA
CGCGGCCACGCAACGCCACCGCATCGATTGGCGCTGGGTGAAAGGCCACAATGGCGACCCGGACAACGAGCGCGTCGACG
TGCTCGCCCGCAATCAGGCCACCGCCCAGCGCGATGGCCGTGCAACATCGTAA

Upstream 100 bases:

>100_bases
CGCTGCCGCAGCGATCGGCCCGACACCGATTGCGCTGCCGGCCCGCTGGCCGCAACCAGTGGCCACGATCTGAGCTGTTT
CCGTTCAAACCTGGAACCTG

Downstream 100 bases:

>100_bases
GAGGACACATGCGTCAGATCATTCTCGATACCGAAACCACCGGCCTGGAATGGCGCAAGGGCAACCGCGTCGTCGAAATC
GGTGCGGTGGAGCTGCTGGA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKSIEVHTDGSCLGNPGPGGWAALLRYNGREKELAGGEAVSTNNRMELMAAIMALETLTEPCEIVLHTDSQYVRQGITEW
MPGWVRRNWKTAGGDPVKNRELWERLHAATQRHRIDWRWVKGHNGDPDNERVDVLARNQATAQRDGRATS

Sequences:

>Translated_150_residues
MKSIEVHTDGSCLGNPGPGGWAALLRYNGREKELAGGEAVSTNNRMELMAAIMALETLTEPCEIVLHTDSQYVRQGITEW
MPGWVRRNWKTAGGDPVKNRELWERLHAATQRHRIDWRWVKGHNGDPDNERVDVLARNQATAQRDGRATS
>Mature_150_residues
MKSIEVHTDGSCLGNPGPGGWAALLRYNGREKELAGGEAVSTNNRMELMAAIMALETLTEPCEIVLHTDSQYVRQGITEW
MPGWVRRNWKTAGGDPVKNRELWERLHAATQRHRIDWRWVKGHNGDPDNERVDVLARNQATAQRDGRATS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=36.734693877551, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=140, Percent_Identity=63.5714285714286, Blast_Score=192, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=32.8571428571429, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137476, Length=141, Percent_Identity=36.8794326241135, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552509, Length=141, Percent_Identity=36.8794326241135, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16900; Mature: 16900

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIEVHTDGSCLGNPGPGGWAALLRYNGREKELAGGEAVSTNNRMELMAAIMALETLTE
CCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCC
PCEIVLHTDSQYVRQGITEWMPGWVRRNWKTAGGDPVKNRELWERLHAATQRHRIDWRWV
CEEEEEECCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGHNGDPDNERVDVLARNQATAQRDGRATS
CCCCCCCCCCEEEEEECCCCHHHCCCCCCC
>Mature Secondary Structure
MKSIEVHTDGSCLGNPGPGGWAALLRYNGREKELAGGEAVSTNNRMELMAAIMALETLTE
CCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCC
PCEIVLHTDSQYVRQGITEWMPGWVRRNWKTAGGDPVKNRELWERLHAATQRHRIDWRWV
CEEEEEECCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGHNGDPDNERVDVLARNQATAQRDGRATS
CCCCCCCCCCEEEEEECCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]