Click here to switch to the map view.

The map label for this gene is tig [H]

Identifier: 84622588

GI number: 84622588

Start: 1015393

End: 1016685

Strand: Direct

Name: tig [H]

Synonym: XOO_0931

Alternate gene names: 84622588

Gene position: 1015393-1016685 (Clockwise)

Preceding gene: 84622587

Following gene: 84622589

Centisome position: 20.55

GC content: 64.58

Gene sequence:

>1293_bases
ATGCAAGCATCGATCGAATCGACCGGCAATCTGGAACGCCGCCTGACCTTCACCCTGCCGCAGGAGCGCCTGGAGACGCA
CGTCGGTGGCCGTCTGCGCGAGCTTGCGCGAACCACGCGCATCAAGGGGTTCCGCCCCGGCAAGGTGCCGACCAAGGTCA
TCGAGCAGCGTTTTGGCCAGCAGGTACGTGCCGAAGCGATGGAAGGCCTGCTGCGTGAGACCTTCGATTCGGCGGTGCGC
GAGCATTCGCTGCGCCTGGCCGGCAACCCGCGCATCGACCAGGGCGAGACCGATTTCGACTTCGTCGCCACCTTCGAAGT
GGTGCCGGATTTCGGCGACATCGACGTGACCACGCTGTCGGTGGTGCGTGCGACCGCTGAAGTGACCGATGCCGATATCG
ATCAGATGATCGAAAACCTGCGCCTGCAGCGTCGTATCTGGAATCCGGTCGAGCGCGGTGCGCAGGCCGGCGACCTCGTG
GCGCTGGAGACCTGGTCGCAGGCCGGCGACGAGCGTCTGCCCGCCGACGGCGTAGAAACCGGTAGCAGCGTGCTGGGCTC
CGGCGTCATGTTCGACCAGATCGAAAAGGGTCTGGAAGGCCTGACAAAGGGCGAAGAAAAGACCCTGAGCGTCGATTTCC
CGGCCGAGTGGCGAGTGCCGCAGCTGGCGGGCAAGACCGTGCAGGTGCACGTCAAGGCGGTCGAAGTGTCCGAGCCGGTG
TTACCGGCAGTGGACAAGGAGTTCATCAAAAGCTTTGGCGTGAAGAGTGGCGACGCCGAGCAGTTCCGCGCCGACATTCG
CACCAACCTGGAGCGCGAGCTCAAGGGTGCGCTGATGAATCGTCTGCGCCGCGAAGTGGGCGAGCAGCTGATCGCTGCAT
ATGCGCACGTCGAAATGCCGCCGCGCCTGGTCGAGAACGAAGCCCGTTCGATGCTGGCCCAGCAGGTCGAGCAGGTCCGC
CGCAGCGGCCGCGATCCGGGTCAGGTGCCGGCCGATGCGCATCAGGGGTTCATGGACGCCGCCGCCAAGCGCGTGCTGGT
CGGTCTGCTGGTGGGTGAAGTGGCGCGCCGCAATGAATTGCGCCTGGAATCCAGGCGCGTCAGCGACACGCTGCGTCTGA
TCGCTTCGACCTACGAAGAGCCGGAACAGGTCATTGAGATGTACCGCAACGACCCCCAACTGATGAATGGACTGCAGAGC
CGCGTGATGAAAGAGCAGGTGATCGACTGGATCGCCGAGCGCGCCCAACACACCGAGCAGTCGCTGTCGTTCCAGGACGC
CATCCGCGTCTGA

Upstream 100 bases:

>100_bases
TATCGGCGAAACTATAAGGCTCGGCGGCTTTCTCGCCATCCGTCAACCCGCATCTTCCATCTCGTGCCGACGCTGTGCGC
TGTCGGTGGCAGGAGTCAAC

Downstream 100 bases:

>100_bases
TCCGGCAGTAAGAGAAGAACCGCGCCCAGCGTCAGGGGTGCGGATGTCGAACACGGGCAGCGGCACAGGCCGCTGCCACA
ACAGGAAGACTGATGAGCAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MQASIESTGNLERRLTFTLPQERLETHVGGRLRELARTTRIKGFRPGKVPTKVIEQRFGQQVRAEAMEGLLRETFDSAVR
EHSLRLAGNPRIDQGETDFDFVATFEVVPDFGDIDVTTLSVVRATAEVTDADIDQMIENLRLQRRIWNPVERGAQAGDLV
ALETWSQAGDERLPADGVETGSSVLGSGVMFDQIEKGLEGLTKGEEKTLSVDFPAEWRVPQLAGKTVQVHVKAVEVSEPV
LPAVDKEFIKSFGVKSGDAEQFRADIRTNLERELKGALMNRLRREVGEQLIAAYAHVEMPPRLVENEARSMLAQQVEQVR
RSGRDPGQVPADAHQGFMDAAAKRVLVGLLVGEVARRNELRLESRRVSDTLRLIASTYEEPEQVIEMYRNDPQLMNGLQS
RVMKEQVIDWIAERAQHTEQSLSFQDAIRV

Sequences:

>Translated_430_residues
MQASIESTGNLERRLTFTLPQERLETHVGGRLRELARTTRIKGFRPGKVPTKVIEQRFGQQVRAEAMEGLLRETFDSAVR
EHSLRLAGNPRIDQGETDFDFVATFEVVPDFGDIDVTTLSVVRATAEVTDADIDQMIENLRLQRRIWNPVERGAQAGDLV
ALETWSQAGDERLPADGVETGSSVLGSGVMFDQIEKGLEGLTKGEEKTLSVDFPAEWRVPQLAGKTVQVHVKAVEVSEPV
LPAVDKEFIKSFGVKSGDAEQFRADIRTNLERELKGALMNRLRREVGEQLIAAYAHVEMPPRLVENEARSMLAQQVEQVR
RSGRDPGQVPADAHQGFMDAAAKRVLVGLLVGEVARRNELRLESRRVSDTLRLIASTYEEPEQVIEMYRNDPQLMNGLQS
RVMKEQVIDWIAERAQHTEQSLSFQDAIRV
>Mature_430_residues
MQASIESTGNLERRLTFTLPQERLETHVGGRLRELARTTRIKGFRPGKVPTKVIEQRFGQQVRAEAMEGLLRETFDSAVR
EHSLRLAGNPRIDQGETDFDFVATFEVVPDFGDIDVTTLSVVRATAEVTDADIDQMIENLRLQRRIWNPVERGAQAGDLV
ALETWSQAGDERLPADGVETGSSVLGSGVMFDQIEKGLEGLTKGEEKTLSVDFPAEWRVPQLAGKTVQVHVKAVEVSEPV
LPAVDKEFIKSFGVKSGDAEQFRADIRTNLERELKGALMNRLRREVGEQLIAAYAHVEMPPRLVENEARSMLAQQVEQVR
RSGRDPGQVPADAHQGFMDAAAKRVLVGLLVGEVARRNELRLESRRVSDTLRLIASTYEEPEQVIEMYRNDPQLMNGLQS
RVMKEQVIDWIAERAQHTEQSLSFQDAIRV

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=430, Percent_Identity=34.4186046511628, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48229; Mature: 48229

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASIESTGNLERRLTFTLPQERLETHVGGRLRELARTTRIKGFRPGKVPTKVIEQRFGQ
CCCCCCCCCCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVRAEAMEGLLRETFDSAVREHSLRLAGNPRIDQGETDFDFVATFEVVPDFGDIDVTTLS
HHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCHHHEEEEEECCCCCCCCHHHHH
VVRATAEVTDADIDQMIENLRLQRRIWNPVERGAQAGDLVALETWSQAGDERLPADGVET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCC
GSSVLGSGVMFDQIEKGLEGLTKGEEKTLSVDFPAEWRVPQLAGKTVQVHVKAVEVSEPV
CHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCCEEEEEEEEEECCCCC
LPAVDKEFIKSFGVKSGDAEQFRADIRTNLERELKGALMNRLRREVGEQLIAAYAHVEMP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRLVENEARSMLAQQVEQVRRSGRDPGQVPADAHQGFMDAAAKRVLVGLLVGEVARRNEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLESRRVSDTLRLIASTYEEPEQVIEMYRNDPQLMNGLQSRVMKEQVIDWIAERAQHTEQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSFQDAIRV
HCCHHHHHCC
>Mature Secondary Structure
MQASIESTGNLERRLTFTLPQERLETHVGGRLRELARTTRIKGFRPGKVPTKVIEQRFGQ
CCCCCCCCCCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVRAEAMEGLLRETFDSAVREHSLRLAGNPRIDQGETDFDFVATFEVVPDFGDIDVTTLS
HHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCHHHEEEEEECCCCCCCCHHHHH
VVRATAEVTDADIDQMIENLRLQRRIWNPVERGAQAGDLVALETWSQAGDERLPADGVET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCC
GSSVLGSGVMFDQIEKGLEGLTKGEEKTLSVDFPAEWRVPQLAGKTVQVHVKAVEVSEPV
CHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCCEEEEEEEEEECCCCC
LPAVDKEFIKSFGVKSGDAEQFRADIRTNLERELKGALMNRLRREVGEQLIAAYAHVEMP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRLVENEARSMLAQQVEQVRRSGRDPGQVPADAHQGFMDAAAKRVLVGLLVGEVARRNEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLESRRVSDTLRLIASTYEEPEQVIEMYRNDPQLMNGLQSRVMKEQVIDWIAERAQHTEQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSFQDAIRV
HCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]