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The map label for this gene is insK [C]

Identifier: 84622554

GI number: 84622554

Start: 983297

End: 984112

Strand: Direct

Name: insK [C]

Synonym: XOO_0897

Alternate gene names: 84622554

Gene position: 983297-984112 (Clockwise)

Preceding gene: 84622553

Following gene: 84622555

Centisome position: 19.9

GC content: 64.09

Gene sequence:

>816_bases
GTGCGCGAGTGGATCGAAGGTGGCGCCAGCGAGCGCTGCGCCCTGGCAGCGATCGGCATGAGCGCCAGTGCGCTGCGCTA
TCGCCCGCGCGAGGACCGCAACGTTGAGTTGCGCGAGCGCATCCTTGCGTTGGCGCATCGCCATCGCCGCTATGGCGTGG
GGATGATCTCTCTCAAGCTGCGGCAGGAAGGGCGTCTCGTGAACTATAAGCGGGTGGAGCGGCTGTATTGCGAGCAGCAG
CTGCAGGTCCGCCGCCGCACGCGTAAAAAGGTGCCGCTAGGCGAGCGTGCGCCGTTGCTGCGGCCCACCAAGGCCAACCC
GGTGTGGTCGATGGACGTCGTGTTCGACCGCACCGCCGAAGGTCGGGCGATCAAGTCTCTGGTGATCGTGGACGACGCGA
CACACCAGGCAGTCGCAATCGACGTGGAACGCGCCATCTCCGGCCACCGGGTAGCACGCGTGCTGGATCGGTTGGCACAC
AGCGGCGGCCTGCCGCAGGTGATCCGCACGGACAATGGCAAGGAGTTCTGTGGCAAGGCCATGGTCGCCTGGGCGCATGC
CAATCGTGTGCAGCTACGCCAGATCCAGCCTGGCAAGCCGAACCAGAATGCCTACGTCGAATCCTTCAACGGCCGGCTAC
GCGACGAATGCCTCAACGAACACTGGTTCCCAACGCTGCTGCATGCGCGCACCGAGATCGAACGCTGGCGCCGCGAATAC
AACCAACACCGCCCAAAGAAAGCAATCGGCGCAATGACGCCGGCAACGTATGCCCAGCAGTTGGCCAATAGCGATATCAT
CAACCCCGGACTCTAA

Upstream 100 bases:

>100_bases
CGCGACTGAAGAAGTTGCTGGCCGAGCAGGTGTTCCAGAACGACCTGATCAAGGATGCGCTGCAAAAACAATGGTGAGCG
CACCGGCGCGTCGTGCGCTG

Downstream 100 bases:

>100_bases
ACCCGACTGCTACTCAGGATGGGGGGACGTCGGGCGCACACGATCAACACCGCTCACGCGATGAAGGCGGTGGATCCACG
TGAGTTGTCGCGGGTGATCG

Product: ISXoo3 transposase orfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MREWIEGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANPVWSMDVVFDRTAEGRAIKSLVIVDDATHQAVAIDVERAISGHRVARVLDRLAH
SGGLPQVIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NQHRPKKAIGAMTPATYAQQLANSDIINPGL

Sequences:

>Translated_271_residues
MREWIEGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANPVWSMDVVFDRTAEGRAIKSLVIVDDATHQAVAIDVERAISGHRVARVLDRLAH
SGGLPQVIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NQHRPKKAIGAMTPATYAQQLANSDIINPGL
>Mature_271_residues
MREWIEGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANPVWSMDVVFDRTAEGRAIKSLVIVDDATHQAVAIDVERAISGHRVARVLDRLAH
SGGLPQVIRTDNGKEFCGKAMVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NQHRPKKAIGAMTPATYAQQLANSDIINPGL

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=244, Percent_Identity=22.9508196721311, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31103; Mature: 31103

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREWIEGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCHHCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPLGERAPLLRPTKANPVWSMDVVFDRTAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHEEEECCCC
GRAIKSLVIVDDATHQAVAIDVERAISGHRVARVLDRLAHSGGLPQVIRTDNGKEFCGKA
CCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
MVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
HHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NQHRPKKAIGAMTPATYAQQLANSDIINPGL
HHCCCHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MREWIEGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCHHCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPLGERAPLLRPTKANPVWSMDVVFDRTAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHEEEECCCC
GRAIKSLVIVDDATHQAVAIDVERAISGHRVARVLDRLAHSGGLPQVIRTDNGKEFCGKA
CCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
MVAWAHANRVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
HHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NQHRPKKAIGAMTPATYAQQLANSDIINPGL
HHCCCHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]