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The map label for this gene is waaA [H]

Identifier: 84622507

GI number: 84622507

Start: 926899

End: 928215

Strand: Direct

Name: waaA [H]

Synonym: XOO_0850

Alternate gene names: 84622507

Gene position: 926899-928215 (Clockwise)

Preceding gene: 84622505

Following gene: 84622511

Centisome position: 18.76

GC content: 65.0

Gene sequence:

>1317_bases
ATGCGGAAAGACCCGATCGAATGGCTGTTGCGCGGGCTGTACTCGGCGCTGCTCTACCTGTTGTTGCCGGTCACGGTGTA
CCACCTGGTGTGGCGCGGCTTTCGTGTGCGCGAATACTTCAATCGCTGGAACGAGCGCTACGCGTCCTACACGCACGCGT
GCGGGCGCCCGCGCGTGTGGGTGCATGCGGTGTCGGTGGGCGAGGTTAACGCGGCCGCGCCGCTGGTCAATGCGCTAAGT
GCGCAGCGCCCGGATATTCGCTGGGTCATCACCACCATCACCCCGACCGGCTCCGAGCGGGTACGCGCGATGTGGGGCGA
TGCGGTGGACCACGTGTATCTGCCGTACGATGTGCCGGGCAGCGTGGGACGATTTCTTGAACACTTCCGCCCGCGTCTGG
CATTGATCCTGGAAACCGAGCTATGGCCGAACATGCTGTTCGGTTGTCGCGACCGCCAGATTCCGGTGTACATCCTCAAT
GCGCGGCTGTCGGCGCGTTCGCTGCGTGGCTATCGGCTGCTGGCGCCATTGATCGGTCGCGCGCTGCAAACTGTCACGTG
CGTGGCTGCGCAATCGCAGGACGATGCCGAGCGCTTCATCACGCTGGGCGCCCGTCCGGATCAGGTGATCGCGCTGGGCA
ATCTCAAATTCGATATCGCCGCGCCGGCGCAATTGCCGGCGCTGGTCGCGCAGTTCCGCACGCACGTGCCGGCTACGCGC
CCAGTGTGGATCGCCGCCAGCACGCACGAAGGCGAAGAGGCTGCCGTTGCCGACATCCACGCTCGCTTGCTGCTGCAATT
TCCAGACTTGCTGTTGTTGTGGGCACCGCGCCATCCCGAGCGCTTTCCCAAGGTCGAGACATTGGCACGCGAGCGCGGCT
GGCGCGTTGCCATCCGCAAGGCACAGCAGTGGCCACAGGCAAGCGACAAAGTCTTCGTCATCGATACCCTGGGCGAGCTG
ATGAGCTTTTATGCGTGCGCGCAGGTGGCCTTCGTCGGCGGCAGCCTGCAGCCGATCGGTGGGCACAATCTGCTCGAACC
CGCTGCGGTCGGCACACCTGCAGTCACCGGCCCGCATCTGCATAATTTCTCGGAGATTTCGCGGCGCATGTGCGAAGCCG
ATGCCGTTGCCATCTGCGAGGATGCCGAGTGCGTATATCAGGCGTTGGCGCGCCTGCTCGCTGATCCCGATCAACGCGAG
GCGATGGCCACCGCTGGCCTGGCATTGGTCGCCAACGGCAAGGGCGCAGTGGCCCGCACGCTGGTGCAGGTCGGCGCGGA
TTTGCCATCGATGGCAAGCGAGGGCGCTGCGGCGTGA

Upstream 100 bases:

>100_bases
GGCGACGTTGGCGGACTCGGGCATGCGCCAATTCTAAGGGATTGCGCCTTGCGGGCAGCGTGCACATGCGCATGCGCTGA
CGCTCCCGTATCCTGCACCG

Downstream 100 bases:

>100_bases
GGTTGTGGTGGTCGGCGCGTTGCTGACCGCATGGGGGCGTGCTGCGATCTCGACGATGCCTGCCGTCAGAGGCAGTGGTC
GTTGCGCTCAGCAGTGAGAA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRKDPIEWLLRGLYSALLYLLLPVTVYHLVWRGFRVREYFNRWNERYASYTHACGRPRVWVHAVSVGEVNAAAPLVNALS
AQRPDIRWVITTITPTGSERVRAMWGDAVDHVYLPYDVPGSVGRFLEHFRPRLALILETELWPNMLFGCRDRQIPVYILN
ARLSARSLRGYRLLAPLIGRALQTVTCVAAQSQDDAERFITLGARPDQVIALGNLKFDIAAPAQLPALVAQFRTHVPATR
PVWIAASTHEGEEAAVADIHARLLLQFPDLLLLWAPRHPERFPKVETLARERGWRVAIRKAQQWPQASDKVFVIDTLGEL
MSFYACAQVAFVGGSLQPIGGHNLLEPAAVGTPAVTGPHLHNFSEISRRMCEADAVAICEDAECVYQALARLLADPDQRE
AMATAGLALVANGKGAVARTLVQVGADLPSMASEGAAA

Sequences:

>Translated_438_residues
MRKDPIEWLLRGLYSALLYLLLPVTVYHLVWRGFRVREYFNRWNERYASYTHACGRPRVWVHAVSVGEVNAAAPLVNALS
AQRPDIRWVITTITPTGSERVRAMWGDAVDHVYLPYDVPGSVGRFLEHFRPRLALILETELWPNMLFGCRDRQIPVYILN
ARLSARSLRGYRLLAPLIGRALQTVTCVAAQSQDDAERFITLGARPDQVIALGNLKFDIAAPAQLPALVAQFRTHVPATR
PVWIAASTHEGEEAAVADIHARLLLQFPDLLLLWAPRHPERFPKVETLARERGWRVAIRKAQQWPQASDKVFVIDTLGEL
MSFYACAQVAFVGGSLQPIGGHNLLEPAAVGTPAVTGPHLHNFSEISRRMCEADAVAICEDAECVYQALARLLADPDQRE
AMATAGLALVANGKGAVARTLVQVGADLPSMASEGAAA
>Mature_438_residues
MRKDPIEWLLRGLYSALLYLLLPVTVYHLVWRGFRVREYFNRWNERYASYTHACGRPRVWVHAVSVGEVNAAAPLVNALS
AQRPDIRWVITTITPTGSERVRAMWGDAVDHVYLPYDVPGSVGRFLEHFRPRLALILETELWPNMLFGCRDRQIPVYILN
ARLSARSLRGYRLLAPLIGRALQTVTCVAAQSQDDAERFITLGARPDQVIALGNLKFDIAAPAQLPALVAQFRTHVPATR
PVWIAASTHEGEEAAVADIHARLLLQFPDLLLLWAPRHPERFPKVETLARERGWRVAIRKAQQWPQASDKVFVIDTLGEL
MSFYACAQVAFVGGSLQPIGGHNLLEPAAVGTPAVTGPHLHNFSEISRRMCEADAVAICEDAECVYQALARLLADPDQRE
AMATAGLALVANGKGAVARTLVQVGADLPSMASEGAAA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=421, Percent_Identity=45.6057007125891, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 48421; Mature: 48421

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDPIEWLLRGLYSALLYLLLPVTVYHLVWRGFRVREYFNRWNERYASYTHACGRPRVW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
VHAVSVGEVNAAAPLVNALSAQRPDIRWVITTITPTGSERVRAMWGDAVDHVYLPYDVPG
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCC
SVGRFLEHFRPRLALILETELWPNMLFGCRDRQIPVYILNARLSARSLRGYRLLAPLIGR
HHHHHHHHHCCCEEEEEECHHCHHHHHCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHHH
ALQTVTCVAAQSQDDAERFITLGARPDQVIALGNLKFDIAAPAQLPALVAQFRTHVPATR
HHHHHHHHHHCCCCCHHHHEECCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHCCCCCC
PVWIAASTHEGEEAAVADIHARLLLQFPDLLLLWAPRHPERFPKVETLARERGWRVAIRK
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHH
AQQWPQASDKVFVIDTLGELMSFYACAQVAFVGGSLQPIGGHNLLEPAAVGTPAVTGPHL
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HNFSEISRRMCEADAVAICEDAECVYQALARLLADPDQREAMATAGLALVANGKGAVART
CCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCHHHHHHHCCEEEEECCCCHHHHH
LVQVGADLPSMASEGAAA
HHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MRKDPIEWLLRGLYSALLYLLLPVTVYHLVWRGFRVREYFNRWNERYASYTHACGRPRVW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
VHAVSVGEVNAAAPLVNALSAQRPDIRWVITTITPTGSERVRAMWGDAVDHVYLPYDVPG
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCC
SVGRFLEHFRPRLALILETELWPNMLFGCRDRQIPVYILNARLSARSLRGYRLLAPLIGR
HHHHHHHHHCCCEEEEEECHHCHHHHHCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHHH
ALQTVTCVAAQSQDDAERFITLGARPDQVIALGNLKFDIAAPAQLPALVAQFRTHVPATR
HHHHHHHHHHCCCCCHHHHEECCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHCCCCCC
PVWIAASTHEGEEAAVADIHARLLLQFPDLLLLWAPRHPERFPKVETLARERGWRVAIRK
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHH
AQQWPQASDKVFVIDTLGELMSFYACAQVAFVGGSLQPIGGHNLLEPAAVGTPAVTGPHL
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HNFSEISRRMCEADAVAICEDAECVYQALARLLADPDQREAMATAGLALVANGKGAVART
CCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCHHHHHHHCCEEEEECCCCHHHHH
LVQVGADLPSMASEGAAA
HHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]