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The map label for this gene is slt [H]

Identifier: 84622403

GI number: 84622403

Start: 804912

End: 807029

Strand: Direct

Name: slt [H]

Synonym: XOO_0746

Alternate gene names: 84622403

Gene position: 804912-807029 (Clockwise)

Preceding gene: 84622400

Following gene: 84622406

Centisome position: 16.29

GC content: 66.43

Gene sequence:

>2118_bases
GTGCCATCGCGGTGGCTACAAGGTGGCACTCGCATGCGCGTGCGTGACGCGCCGCGCCGCGCATGCGAGCGGATGCGGCG
TCTGCGCCAGGTCCGCGATGCGTATTCACTCAGGCGCTCAGGCGTGCTTCGGTATCATCGCGCCATGACTTACGCCCCCC
GCTTCGCCACCGTTTTGATCGGCCTGCTGTGCCTTGGCAGCACCGCGCTGCATGCGCAAACGCTCGACCCGCAATTGACT
GCCATGCGCGATGCCATCGCGGCCGCAGAGCGCGGCCAGACCGATGCCGGTCAGCTCGCCGCATTGAGCCGCCACCCGCT
GTATGGCTGGCTGGAATTTGCCACGCTCAAGCGCAGCATCGACACCGTCTCCAATACGCAGGCGCAGGGGTTTCTACAGC
GCTATGCCGGCCAACCCGTCGCCGAGAGCTTCCGCAGCATCTGGTTGCCGGCAGTGGCGCGTCGACAGGACTGGGACACG
CTGCTGGCCAACTGGAAGTCCACCGACAACGTCGGCCTGCGCTGCGCACAGCTGACCGCGCTCCTGGCCACCAGCAAGGC
GGATGCCCAGTGGAGCCGCGATGCGCTGACGCTGTGGCGTAATGGCAAGGCGCTGCCCGACGCCTGCGATGCGGTGGTGG
CAGGGCTTGAATCGCGCGGCGAACTCACTCCCGCGCTGCGCTGGGAACGCGTGGAAGCCGCTGCCGATGCGCAGCTGCCT
GCGGTCATGCGCAAGGCTGCCCGCGGCCTGCCCGCCGCCGATCTGGCACTGGCCACCGACTACGCTGCATTCCTCGACAA
GGTGCATCCGCGTGCGCTGAGCTGGCCGAAGAGCGTGCGCAGCCGCCGCATCGCGGTCGATGGTCTGGCCAAGCTGGCCA
AGAGCGATCCGGATGCTGCCGAACAGCAGTTGCCGCAGCTCGCCAGCGCGCTTGGTTTCAGTGAGGCGCAGCGCGGACAG
GTGCTGTATCAGATCGCGTTGTGGACCGTCGCCTCGTATCTGCCGGATTCGGCACGTCGGCTCAACGCTGTGCCAGACGC
GTCCTACGATGAGCGCCTGCACGAATGGCGCGCACGCGAGGCAATGTCGCGCGGCGACTGGCCGGCAGCGTTGGCTGCGA
TTCGCAAGATGCCGGCAAGCCAGCGCAACGATTCGCGTTGGCAGTATTTCGAAGCACGCCTCGCCGAAAAGACCGGCGTT
GCCGCGGCCGCACAGCCGCTGTACCGCGCCGCCGCGCAATCGCCGACCTTCCACGGCTTCTTGGCCGCCGACCGCCTGCA
ACAGCCGTACCGGCTGTGCCCGTGGGAGCCCAACGACAGCCCACAGGCGAAAGCGGCCGTTGCGCGCGACCCAGCCTTGA
TTCGCGCGATCGCCTTGTTCCAGATCGACCGCCCCGGCTGGGCCGCGGCCGAATGGAACGATGCAGCCACCCGCTTCGAC
GACACCCAGCGCCGTCTGGCAGTAGAAGTGGCCAGCGACAACGGTTGGTTCGATCGCGCGGTATTCGCGCTCGGCAAGCA
GCCGGACGAACAACGCCTGTATTCGTTGAGATTCCCACTGCACCACGAGGACACCATTCGCCGCGAAGCCGCCAAGAACG
CAATCGACCCGGCCTGGGTGGCCGGAGAAATTCGTGCCGAAAGCATCTTCAATCCGCGCGCGCGCTCACCGGCCAATGCA
ATGGGCCTGATGCAGGTGTTGCCGGGTACCGGCGCAGCCGTCGCCAAGGGCATCGCCCTGCCCGGCTATGCGGGCGCCAC
CAGCCTGTACGAGCCAAACACCAATATCGCCATCGGCACGGCGTATCTGCGCCAGTTGCTCAACACCTATGTGCTGCCCT
ATCTCACCATCGCCGCCTACAACGCGGGTCCCGGCCCCACCGGACGCTGGCAATCGCAACGGCCCAATTTCGATCCGGAC
TTCTGGATCGAGACCATCAGCTACAAGGAAACCCGCGACTACGTTGCACGCGTGTTGGCCTTCAGCGTGATCTACGACTG
GCGCCTCAACGGCGACATGCTGCCGCTGAGCGACCGACTGATGGGCAAGCTGGTGGACAAGCGCCTCAAGCCAGTGTGCA
CACCGGGCCAGGCTGCGAATAACCCGGCGCAGGATTGA

Upstream 100 bases:

>100_bases
CACAGTGGAAGTAAGTGTCGGCACGCCCCCGTGCCCTGCGGTCCGCGCCAATCCTTGAACGAAACATTAACGCATCGCAA
CTGACGGTGCAGCAACGCGC

Downstream 100 bases:

>100_bases
GCCAAGCCACGCCACGTGTGCAGCCGCTGGAATTCGCGGCGCGTTAGGCACTATGGCTGCCGCACAGTACTGACAAAGAA
GTACTTGCAGCGTGTCTTGT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MPSRWLQGGTRMRVRDAPRRACERMRRLRQVRDAYSLRRSGVLRYHRAMTYAPRFATVLIGLLCLGSTALHAQTLDPQLT
AMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFATLKRSIDTVSNTQAQGFLQRYAGQPVAESFRSIWLPAVARRQDWDT
LLANWKSTDNVGLRCAQLTALLATSKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQLP
AVMRKAARGLPAADLALATDYAAFLDKVHPRALSWPKSVRSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSEAQRGQ
VLYQIALWTVASYLPDSARRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPASQRNDSRWQYFEARLAEKTGV
AAAAQPLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSPQAKAAVARDPALIRAIALFQIDRPGWAAAEWNDAATRFD
DTQRRLAVEVASDNGWFDRAVFALGKQPDEQRLYSLRFPLHHEDTIRREAAKNAIDPAWVAGEIRAESIFNPRARSPANA
MGLMQVLPGTGAAVAKGIALPGYAGATSLYEPNTNIAIGTAYLRQLLNTYVLPYLTIAAYNAGPGPTGRWQSQRPNFDPD
FWIETISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNPAQD

Sequences:

>Translated_705_residues
MPSRWLQGGTRMRVRDAPRRACERMRRLRQVRDAYSLRRSGVLRYHRAMTYAPRFATVLIGLLCLGSTALHAQTLDPQLT
AMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFATLKRSIDTVSNTQAQGFLQRYAGQPVAESFRSIWLPAVARRQDWDT
LLANWKSTDNVGLRCAQLTALLATSKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQLP
AVMRKAARGLPAADLALATDYAAFLDKVHPRALSWPKSVRSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSEAQRGQ
VLYQIALWTVASYLPDSARRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPASQRNDSRWQYFEARLAEKTGV
AAAAQPLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSPQAKAAVARDPALIRAIALFQIDRPGWAAAEWNDAATRFD
DTQRRLAVEVASDNGWFDRAVFALGKQPDEQRLYSLRFPLHHEDTIRREAAKNAIDPAWVAGEIRAESIFNPRARSPANA
MGLMQVLPGTGAAVAKGIALPGYAGATSLYEPNTNIAIGTAYLRQLLNTYVLPYLTIAAYNAGPGPTGRWQSQRPNFDPD
FWIETISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNPAQD
>Mature_704_residues
PSRWLQGGTRMRVRDAPRRACERMRRLRQVRDAYSLRRSGVLRYHRAMTYAPRFATVLIGLLCLGSTALHAQTLDPQLTA
MRDAIAAAERGQTDAGQLAALSRHPLYGWLEFATLKRSIDTVSNTQAQGFLQRYAGQPVAESFRSIWLPAVARRQDWDTL
LANWKSTDNVGLRCAQLTALLATSKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQLPA
VMRKAARGLPAADLALATDYAAFLDKVHPRALSWPKSVRSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSEAQRGQV
LYQIALWTVASYLPDSARRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPASQRNDSRWQYFEARLAEKTGVA
AAAQPLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSPQAKAAVARDPALIRAIALFQIDRPGWAAAEWNDAATRFDD
TQRRLAVEVASDNGWFDRAVFALGKQPDEQRLYSLRFPLHHEDTIRREAAKNAIDPAWVAGEIRAESIFNPRARSPANAM
GLMQVLPGTGAAVAKGIALPGYAGATSLYEPNTNIAIGTAYLRQLLNTYVLPYLTIAAYNAGPGPTGRWQSQRPNFDPDF
WIETISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNPAQD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=625, Percent_Identity=27.84, Blast_Score=195, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78176; Mature: 78044

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRWLQGGTRMRVRDAPRRACERMRRLRQVRDAYSLRRSGVLRYHRAMTYAPRFATVLI
CCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
GLLCLGSTALHAQTLDPQLTAMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFATLKRSI
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
DTVSNTQAQGFLQRYAGQPVAESFRSIWLPAVARRQDWDTLLANWKSTDNVGLRCAQLTA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHH
LLATSKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQLP
HHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHH
AVMRKAARGLPAADLALATDYAAFLDKVHPRALSWPKSVRSRRIAVDGLAKLAKSDPDAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCHH
EQQLPQLASALGFSEAQRGQVLYQIALWTVASYLPDSARRLNAVPDASYDERLHEWRARE
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
AMSRGDWPAALAAIRKMPASQRNDSRWQYFEARLAEKTGVAAAAQPLYRAAAQSPTFHGF
HHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHH
LAADRLQQPYRLCPWEPNDSPQAKAAVARDPALIRAIALFQIDRPGWAAAEWNDAATRFD
HHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
DTQRRLAVEVASDNGWFDRAVFALGKQPDEQRLYSLRFPLHHEDTIRREAAKNAIDPAWV
HHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHEEEECCCCCHHHHHHHHHHCCCCHHHH
AGEIRAESIFNPRARSPANAMGLMQVLPGTGAAVAKGIALPGYAGATSLYEPNTNIAIGT
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEHH
AYLRQLLNTYVLPYLTIAAYNAGPGPTGRWQSQRPNFDPDFWIETISYKETRDYVARVLA
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHH
FSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNPAQD
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSRWLQGGTRMRVRDAPRRACERMRRLRQVRDAYSLRRSGVLRYHRAMTYAPRFATVLI
CCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
GLLCLGSTALHAQTLDPQLTAMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFATLKRSI
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
DTVSNTQAQGFLQRYAGQPVAESFRSIWLPAVARRQDWDTLLANWKSTDNVGLRCAQLTA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHH
LLATSKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQLP
HHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHH
AVMRKAARGLPAADLALATDYAAFLDKVHPRALSWPKSVRSRRIAVDGLAKLAKSDPDAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCHH
EQQLPQLASALGFSEAQRGQVLYQIALWTVASYLPDSARRLNAVPDASYDERLHEWRARE
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
AMSRGDWPAALAAIRKMPASQRNDSRWQYFEARLAEKTGVAAAAQPLYRAAAQSPTFHGF
HHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHH
LAADRLQQPYRLCPWEPNDSPQAKAAVARDPALIRAIALFQIDRPGWAAAEWNDAATRFD
HHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
DTQRRLAVEVASDNGWFDRAVFALGKQPDEQRLYSLRFPLHHEDTIRREAAKNAIDPAWV
HHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHEEEECCCCCHHHHHHHHHHCCCCHHHH
AGEIRAESIFNPRARSPANAMGLMQVLPGTGAAVAKGIALPGYAGATSLYEPNTNIAIGT
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEHH
AYLRQLLNTYVLPYLTIAAYNAGPGPTGRWQSQRPNFDPDFWIETISYKETRDYVARVLA
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHH
FSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNPAQD
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]