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The map label for this gene is bioD [H]

Identifier: 84622354

GI number: 84622354

Start: 750106

End: 750780

Strand: Direct

Name: bioD [H]

Synonym: XOO_0697

Alternate gene names: 84622354

Gene position: 750106-750780 (Clockwise)

Preceding gene: 84622351

Following gene: 84622358

Centisome position: 15.18

GC content: 68.15

Gene sequence:

>675_bases
ATGCAGCCTCCGGCGTTTTATGTCACCGGTACCGATACCGGCATCGGCAAAACCATGGGCAGCACTGCGCTGCTGCACGC
GCTGCGTGCACGCGGCCACACCGCAGTGGGCATGAAGCCGGTCGCCAGCGGCTGCGAGCGCACGCCGCAGGGCTGGCGCA
ACGAAGACGCGCTGGCGCTGCAGGCGGCCAGCAATCCGCAGCCGGCGTACGCTACGCTCAACCCCTACGCCTTGCCTGCG
CCGCTGGCACCGGAACTGGCCGCAGCTGACGTCGGCGTGACGCTGTCGCTGGAGCCGATTACGCAGGCCTTCGCGCAATT
GCGCGCGCAGGCCGAGGTCGTGGTGGTGGAGGGCGTCGGTGGTTGGGCCGCGCCGCTGAGCGCAACGTTGGATCAAGCCG
ATCTGGTGCGTGCATTGCAGCTGCCGGTGGTGCTGGTGGTCGGCGTGCGGCTGGGTTGCATCAACCATGCGCGCCTTACC
GCTGCCGCCATTGCCGCCGATGGGCTGCAATGCATCGGCTGGATCGCCAATGAAGTCGACCCGCAGATGGAGCGTGTCGA
AGAGAACATCGGCATGCTGCGCCAGCGTTTGGCAATGCCGTGCTGGGGACGGATCCCGTGGCGGCCGGACGCCGAGGCTG
CTGCTCAGGCTCAGGGACTGCAGCTGCCGCGCTAA

Upstream 100 bases:

>100_bases
GCAGACAAGCCAGAAGCAACCGGCTTCAACAACGTGGCCAATGCGCGCGTTATGCTGCGCGCCCTCACGGCAATGGCGGC
GCGGCCGCAGGACCAGGCAT

Downstream 100 bases:

>100_bases
ATGCCGCTGAGCACAGCGTGCAGGCAATAAAAAAGCCCGACAGAGGGAGGGATCTGTCGGGCCGGATTGCACGGGGGGAG
GGACCCATGCAAAGTCGCTG

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQPPAFYVTGTDTGIGKTMGSTALLHALRARGHTAVGMKPVASGCERTPQGWRNEDALALQAASNPQPAYATLNPYALPA
PLAPELAAADVGVTLSLEPITQAFAQLRAQAEVVVVEGVGGWAAPLSATLDQADLVRALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERVEENIGMLRQRLAMPCWGRIPWRPDAEAAAQAQGLQLPR

Sequences:

>Translated_224_residues
MQPPAFYVTGTDTGIGKTMGSTALLHALRARGHTAVGMKPVASGCERTPQGWRNEDALALQAASNPQPAYATLNPYALPA
PLAPELAAADVGVTLSLEPITQAFAQLRAQAEVVVVEGVGGWAAPLSATLDQADLVRALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERVEENIGMLRQRLAMPCWGRIPWRPDAEAAAQAQGLQLPR
>Mature_224_residues
MQPPAFYVTGTDTGIGKTMGSTALLHALRARGHTAVGMKPVASGCERTPQGWRNEDALALQAASNPQPAYATLNPYALPA
PLAPELAAADVGVTLSLEPITQAFAQLRAQAEVVVVEGVGGWAAPLSATLDQADLVRALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERVEENIGMLRQRLAMPCWGRIPWRPDAEAAAQAQGLQLPR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=218, Percent_Identity=47.7064220183486, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87081944, Length=217, Percent_Identity=42.8571428571429, Blast_Score=171, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324385, Length=180, Percent_Identity=35, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23577; Mature: 23577

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPPAFYVTGTDTGIGKTMGSTALLHALRARGHTAVGMKPVASGCERTPQGWRNEDALAL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEE
QAASNPQPAYATLNPYALPAPLAPELAAADVGVTLSLEPITQAFAQLRAQAEVVVVEGVG
EECCCCCCCEEECCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCC
GWAAPLSATLDQADLVRALQLPVVLVVGVRLGCINHARLTAAAIAADGLQCIGWIANEVD
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PQMERVEENIGMLRQRLAMPCWGRIPWRPDAEAAAQAQGLQLPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MQPPAFYVTGTDTGIGKTMGSTALLHALRARGHTAVGMKPVASGCERTPQGWRNEDALAL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEE
QAASNPQPAYATLNPYALPAPLAPELAAADVGVTLSLEPITQAFAQLRAQAEVVVVEGVG
EECCCCCCCEEECCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCC
GWAAPLSATLDQADLVRALQLPVVLVVGVRLGCINHARLTAAAIAADGLQCIGWIANEVD
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PQMERVEENIGMLRQRLAMPCWGRIPWRPDAEAAAQAQGLQLPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]