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The map label for this gene is ilvB [H]

Identifier: 84489414

GI number: 84489414

Start: 700305

End: 701894

Strand: Direct

Name: ilvB [H]

Synonym: Msp_0605

Alternate gene names: 84489414

Gene position: 700305-701894 (Clockwise)

Preceding gene: 84489413

Following gene: 84489415

Centisome position: 39.62

GC content: 29.87

Gene sequence:

>1590_bases
ATGAAGAAAGATAAAAATATTGCACAGTTTTTAATTGATAAATTAGAATCTAATGGTGTTGAATATGTATTTGGATATCC
TGGAGAACAAGTACTTCCAATATATGAAGCACTACGTAAATCCAATATAAAACATATATTGATGAGACATGAACAGGCAG
CAGCACATGCAGCTGATGCTTATGCAAGAATTACTGGAAAATATGGTGTATGCTTAGCAACAGCAGGACCTGGAGCAATG
AATCTTGTTATGGGTGTATCTGCTGCTTATAAGGATAATGTGCCTTTGATTGTTATAACAGGGGATGTTTCATCAGATGT
TAAGGGTGAAGACACATTTCAAGATATGGATATTAATGCAGTTTTTAAACCAATAACTCTTAAATCATATAATTCAAATA
CACCTGAAAAATTAGAGAATAATATTGAAGAAGTATTTTCATATAATAAACAGGGTATCACTGGTCCATTCCATATTAAT
ATCTCAAAAGATATACAAATTAGGCCAATGAATGTAGACCATAAGGTAATAGAATCAAGAAAAATTAAGTTACCTCTTGA
ATATGATATTAAAGATACAATAAAATCAATAGAAGATGCTAAAAAACCGTTAATAATTGTAGGTTCTGGAATTATTTATG
CAAAGGCATTTGAACAACTAAATACATTCATAGATAAAACAGAGATACCTCTAGTAACAACATACTCTGCAAGGGGAATA
ATACCAGAAACAAATGATAAAAATTTAGGACTAGTTGGAACACGTGGAACAAAAGAAGCAAACTATGCCTCCGAAGAAGC
AGATTTAATACTAGCACTAGGAACAAGACTATCAGATAGAACAAGAAGTCATATAAATACAAGTAACATAATACAAGTAA
ATACAAAAAATCAACAAAAAAATGCAGAGATATTCTATCAAAATCATATAAAAGAATTCTTAGAAGAATTAAATAAAAAT
AAATTACCAAAAACAAGTAAAAATTGGATTGAAGAAATATTATCACAAAAAGACACAACACCAAAAAAATATGCACAAAC
AGAAAAACTACATCCAGAAAAAGCAATACAAACAATACTAAAACAATTAAATGAAAATGTGACAATAACTCTAGATGCTG
GAACAACACCAACATACTTTACAATAGATTCAAAACTCAATAAACATTCACAAATGCTATTTCCAGGGGGTCTTGGACCA
ATGGGATATTCCCTACCTGCAAGTATAGGAGCATCATTTGCAAGACCAGATGATATAATCTTTGCAACAACAAGTGATGG
TTCAGTACAAATGACAATAGAAGAATTAGCTGTAATAAGCACATATCAACTACCAATAATAATATTCATTATAAACAACA
ATGTACTTGGAATAATAAAACAATGGCAAGAAATGGCCAATACTCCTAAATATCAAGTGGATTTAAAAAATCCTGACTTT
GTACAAATAGCAAATGCTTATAGTATTGAAGCAGACAATATAACATCACTTAAAACTCTTGAAACAAAGTTAGAAGAAGC
TATAAAAGATAGAAAACCACATTTATTCAATATAAATGTTGAAGATATACCAATTCCACTACCAAAATAG

Upstream 100 bases:

>100_bases
AAAAAGGTGAAAGAATAGCTCAAGCAGTTGTATTTCCAGTAGAAGGATCTGGTAAATATAATGGATCTTATCAGGAAAAA
TAAGTTAGTGGAGTGTTTTT

Downstream 100 bases:

>100_bases
GTGTTTAAACCCCATAACTACCTTTAGGGGTAGTTATGAAATTATCTCTAGAATAAATTTATCTCAACTCCTCTTTATTT
GATTTAATTTTAAACAAAAC

Product: thiamine pyrophosphate-requiring enzyme

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKKDKNIAQFLIDKLESNGVEYVFGYPGEQVLPIYEALRKSNIKHILMRHEQAAAHAADAYARITGKYGVCLATAGPGAM
NLVMGVSAAYKDNVPLIVITGDVSSDVKGEDTFQDMDINAVFKPITLKSYNSNTPEKLENNIEEVFSYNKQGITGPFHIN
ISKDIQIRPMNVDHKVIESRKIKLPLEYDIKDTIKSIEDAKKPLIIVGSGIIYAKAFEQLNTFIDKTEIPLVTTYSARGI
IPETNDKNLGLVGTRGTKEANYASEEADLILALGTRLSDRTRSHINTSNIIQVNTKNQQKNAEIFYQNHIKEFLEELNKN
KLPKTSKNWIEEILSQKDTTPKKYAQTEKLHPEKAIQTILKQLNENVTITLDAGTTPTYFTIDSKLNKHSQMLFPGGLGP
MGYSLPASIGASFARPDDIIFATTSDGSVQMTIEELAVISTYQLPIIIFIINNNVLGIIKQWQEMANTPKYQVDLKNPDF
VQIANAYSIEADNITSLKTLETKLEEAIKDRKPHLFNINVEDIPIPLPK

Sequences:

>Translated_529_residues
MKKDKNIAQFLIDKLESNGVEYVFGYPGEQVLPIYEALRKSNIKHILMRHEQAAAHAADAYARITGKYGVCLATAGPGAM
NLVMGVSAAYKDNVPLIVITGDVSSDVKGEDTFQDMDINAVFKPITLKSYNSNTPEKLENNIEEVFSYNKQGITGPFHIN
ISKDIQIRPMNVDHKVIESRKIKLPLEYDIKDTIKSIEDAKKPLIIVGSGIIYAKAFEQLNTFIDKTEIPLVTTYSARGI
IPETNDKNLGLVGTRGTKEANYASEEADLILALGTRLSDRTRSHINTSNIIQVNTKNQQKNAEIFYQNHIKEFLEELNKN
KLPKTSKNWIEEILSQKDTTPKKYAQTEKLHPEKAIQTILKQLNENVTITLDAGTTPTYFTIDSKLNKHSQMLFPGGLGP
MGYSLPASIGASFARPDDIIFATTSDGSVQMTIEELAVISTYQLPIIIFIINNNVLGIIKQWQEMANTPKYQVDLKNPDF
VQIANAYSIEADNITSLKTLETKLEEAIKDRKPHLFNINVEDIPIPLPK
>Mature_529_residues
MKKDKNIAQFLIDKLESNGVEYVFGYPGEQVLPIYEALRKSNIKHILMRHEQAAAHAADAYARITGKYGVCLATAGPGAM
NLVMGVSAAYKDNVPLIVITGDVSSDVKGEDTFQDMDINAVFKPITLKSYNSNTPEKLENNIEEVFSYNKQGITGPFHIN
ISKDIQIRPMNVDHKVIESRKIKLPLEYDIKDTIKSIEDAKKPLIIVGSGIIYAKAFEQLNTFIDKTEIPLVTTYSARGI
IPETNDKNLGLVGTRGTKEANYASEEADLILALGTRLSDRTRSHINTSNIIQVNTKNQQKNAEIFYQNHIKEFLEELNKN
KLPKTSKNWIEEILSQKDTTPKKYAQTEKLHPEKAIQTILKQLNENVTITLDAGTTPTYFTIDSKLNKHSQMLFPGGLGP
MGYSLPASIGASFARPDDIIFATTSDGSVQMTIEELAVISTYQLPIIIFIINNNVLGIIKQWQEMANTPKYQVDLKNPDF
VQIANAYSIEADNITSLKTLETKLEEAIKDRKPHLFNINVEDIPIPLPK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=26.4285714285714, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI21361361, Length=494, Percent_Identity=22.2672064777328, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=32.4074074074074, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1790104, Length=533, Percent_Identity=31.5196998123827, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1786717, Length=520, Percent_Identity=25.7692307692308, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=22.1189591078067, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=24.2258652094718, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=561, Percent_Identity=22.4598930481283, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=476, Percent_Identity=25.4201680672269, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=476, Percent_Identity=25.4201680672269, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=29.2114695340502, Blast_Score=247, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6320588, Length=528, Percent_Identity=23.1060606060606, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=512, Percent_Identity=22.8515625, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=514, Percent_Identity=24.5136186770428, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.1075697211155, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320816, Length=478, Percent_Identity=23.4309623430962, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922626, Length=479, Percent_Identity=27.1398747390397, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59127; Mature: 59127

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDKNIAQFLIDKLESNGVEYVFGYPGEQVLPIYEALRKSNIKHILMRHEQAAAHAADA
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YARITGKYGVCLATAGPGAMNLVMGVSAAYKDNVPLIVITGDVSSDVKGEDTFQDMDINA
HHEEECCCCEEEEECCCCHHHHHHHCHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCCCE
VFKPITLKSYNSNTPEKLENNIEEVFSYNKQGITGPFHINISKDIQIRPMNVDHKVIESR
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCHHHHCCC
KIKLPLEYDIKDTIKSIEDAKKPLIIVGSGIIYAKAFEQLNTFIDKTEIPLVTTYSARGI
EEEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
IPETNDKNLGLVGTRGTKEANYASEEADLILALGTRLSDRTRSHINTSNIIQVNTKNQQK
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCCC
NAEIFYQNHIKEFLEELNKNKLPKTSKNWIEEILSQKDTTPKKYAQTEKLHPEKAIQTIL
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
KQLNENVTITLDAGTTPTYFTIDSKLNKHSQMLFPGGLGPMGYSLPASIGASFARPDDII
HHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEE
FATTSDGSVQMTIEELAVISTYQLPIIIFIINNNVLGIIKQWQEMANTPKYQVDLKNPDF
EEECCCCCEEEEHHHHHHHEEECCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCE
VQIANAYSIEADNITSLKTLETKLEEAIKDRKPHLFNINVEDIPIPLPK
EEEECEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKDKNIAQFLIDKLESNGVEYVFGYPGEQVLPIYEALRKSNIKHILMRHEQAAAHAADA
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YARITGKYGVCLATAGPGAMNLVMGVSAAYKDNVPLIVITGDVSSDVKGEDTFQDMDINA
HHEEECCCCEEEEECCCCHHHHHHHCHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCCCE
VFKPITLKSYNSNTPEKLENNIEEVFSYNKQGITGPFHINISKDIQIRPMNVDHKVIESR
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCHHHHCCC
KIKLPLEYDIKDTIKSIEDAKKPLIIVGSGIIYAKAFEQLNTFIDKTEIPLVTTYSARGI
EEEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
IPETNDKNLGLVGTRGTKEANYASEEADLILALGTRLSDRTRSHINTSNIIQVNTKNQQK
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCCC
NAEIFYQNHIKEFLEELNKNKLPKTSKNWIEEILSQKDTTPKKYAQTEKLHPEKAIQTIL
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
KQLNENVTITLDAGTTPTYFTIDSKLNKHSQMLFPGGLGPMGYSLPASIGASFARPDDII
HHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEE
FATTSDGSVQMTIEELAVISTYQLPIIIFIINNNVLGIIKQWQEMANTPKYQVDLKNPDF
EEECCCCCEEEEHHHHHHHEEECCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCE
VQIANAYSIEADNITSLKTLETKLEEAIKDRKPHLFNINVEDIPIPLPK
EEEECEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]