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The map label for this gene is 84489294

Identifier: 84489294

GI number: 84489294

Start: 566762

End: 567250

Strand: Direct

Name: 84489294

Synonym: Msp_0483

Alternate gene names: NA

Gene position: 566762-567250 (Clockwise)

Preceding gene: 84489293

Following gene: 84489305

Centisome position: 32.07

GC content: 22.9

Gene sequence:

>489_bases
TTGGGTAATTGTGTAATTTTCTTAGATGAAAAAAGAATTCCTTTGAATCAACAAATACGAAGTCAAATGTTATCTAAATA
CCCATATTATGGTGCTGCAGGAATCATTGATTATGTGAATAATTATATTTTTGATGATGAATTAATATTATTAGGTGAAG
ATGGTTCTATTATTCCCACATTAGCTTCAGGAAAATGTTGGGTTAGTAATCATGCACATGTACTTAAAAATAAAAAAAAT
ATCAATTTATATTTCTTATATAATATTTTATCTAAAATTCATTTTGAAAAATACAATACTGGTACTATCCAACCAAAATT
AAACAAAAAAACGGCAAAAAACATCAAAATTAAAATAACTTCCAAAAAAGAACAAGAAAAAATTGTTGATTTTATGTTAT
CAATAGGAACAAAAATAAAAAAAATTCAAAAACAGATAAAATTTCTAAAAACGTTCAAAAAAGGATTATTACAGAAAATG
TTTTGTTAA

Upstream 100 bases:

>100_bases
TATAAAGAAATATTATTTAGAAAATCTATTTCCAAAAGAAAATGAAACTATACCTAATCTACGGTTTTTAGAATTTAACA
ATGATTGGATAAAAACTACA

Downstream 100 bases:

>100_bases
ACAAACATCTTTTGAAGTAAATCCTTTTTATATCTTTTTTCATAATTTATTTTCTTACTTAAATATTCTAATTTAATATC
AATATTAGATAATATATTAA

Product: hypothetical protein

Products: NA

Alternate protein names: S protein [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGNCVIFLDEKRIPLNQQIRSQMLSKYPYYGAAGIIDYVNNYIFDDELILLGEDGSIIPTLASGKCWVSNHAHVLKNKKN
INLYFLYNILSKIHFEKYNTGTIQPKLNKKTAKNIKIKITSKKEQEKIVDFMLSIGTKIKKIQKQIKFLKTFKKGLLQKM
FC

Sequences:

>Translated_162_residues
MGNCVIFLDEKRIPLNQQIRSQMLSKYPYYGAAGIIDYVNNYIFDDELILLGEDGSIIPTLASGKCWVSNHAHVLKNKKN
INLYFLYNILSKIHFEKYNTGTIQPKLNKKTAKNIKIKITSKKEQEKIVDFMLSIGTKIKKIQKQIKFLKTFKKGLLQKM
FC
>Mature_161_residues
GNCVIFLDEKRIPLNQQIRSQMLSKYPYYGAAGIIDYVNNYIFDDELILLGEDGSIIPTLASGKCWVSNHAHVLKNKKNI
NLYFLYNILSKIHFEKYNTGTIQPKLNKKTAKNIKIKITSKKEQEKIVDFMLSIGTKIKKIQKQIKFLKTFKKGLLQKMF
C

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 18782; Mature: 18650

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNCVIFLDEKRIPLNQQIRSQMLSKYPYYGAAGIIDYVNNYIFDDELILLGEDGSIIPT
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCEEEECCCCCEEEE
LASGKCWVSNHAHVLKNKKNINLYFLYNILSKIHFEKYNTGTIQPKLNKKTAKNIKIKIT
ECCCEEEECCCHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
SKKEQEKIVDFMLSIGTKIKKIQKQIKFLKTFKKGLLQKMFC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNCVIFLDEKRIPLNQQIRSQMLSKYPYYGAAGIIDYVNNYIFDDELILLGEDGSIIPT
CCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCEEEECCCCCEEEE
LASGKCWVSNHAHVLKNKKNINLYFLYNILSKIHFEKYNTGTIQPKLNKKTAKNIKIKIT
ECCCEEEECCCHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
SKKEQEKIVDFMLSIGTKIKKIQKQIKFLKTFKKGLLQKMFC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]