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The map label for this gene is hsdM [H]

Identifier: 84489290

GI number: 84489290

Start: 562819

End: 564345

Strand: Direct

Name: hsdM [H]

Synonym: Msp_0479

Alternate gene names: 84489290

Gene position: 562819-564345 (Clockwise)

Preceding gene: 84489289

Following gene: 84489291

Centisome position: 31.84

GC content: 33.6

Gene sequence:

>1527_bases
ATGTCAAATTATGAAACAAAATTATGGGCAATAGCAGATAAACTAAGAGGAAATATGGATGCAAACGAATTCAAAAACTA
CATGTTAGGGTTCATATTTTACCGATACCTCTCAGAAAAACTGGAAATGACACTCAACGAATTACTTGAAGAAGATGGAA
TAAACTTCCAAGAAGCATACCAGGATGAAGAACTCATAGAAGACCTAAAAGAAGAAGGAATCGAAAAACTAGGATACTTT
ATACAACCAAAATACCTCTTCAGCAGTGTCATCAATGAAATTGATAAAGGAAGAGAAATACTTGAATGTTTAAGCAATGC
ATTCATAGAAATCAACGATTCATCGTTCAACACAGAAAGTCAGGACGACTTCCAAAACCTATTTGAAGATGTAGATTTAA
ATTCATCCAAATTGGGAAATACCAATGCAGAAAAAAACAAATTAATCTCAGGTATACTACAAGACATTAGTGATATAGAC
TTTGAGTTAGAAAAAGATAATTCTGATATACTTGGTGATGCATATGAATATCTTATCAGTCAATTTGCATCAAGTGCAGG
TAAAAAAGCAGGAGAATTCTACACACCACAAGAAGTATCAACCATACTTGCAAGAATAGTAACACTAAACAAAACCAGAC
TCAAAAGTGTCTATGACCCAACATGTGGAAGTGGATCATTACTTCTAAGAGTAAGTAAAGAAGCAGATGTATCAGAATTC
TATGGACAAGAACTAAACCAGACAACATACAACCTAGCAAGAATGAACATGATACTACACGGAGTCAAATACAACCACTT
CAACATAAAACAGGGAGATTCACTTGAAAATGACAGACACGAAGAGCTTAAATTTGATGCTGTAGTAGCAAATCCACCAT
TTAGTGCAAAATGGAGCAGTGACAAATCATTCATAAATGATGAAAGATTCTCCGGATACGGAAAACTAGCACCAAAAAGT
AAAGCAGACTATGCATTTGTACAGCACATGATATACCACCTCAACGAACAGGGAACACTAGCAGTAGTACTGCCACACGG
AGTATTATTTAGAGGAGCAGCAGAAGGCACCATAAGAAAATACCTAATCAAAGAACTAAACTACCTAGATGCTGTAATAG
GATTACCAAAAAATATCTTCTATGGAACTAGCATACCAACATGCATACTCGTATTCAAAAAATGTCGTGAAGAAGATGAC
AATATACTCTTCATAGATGCATCAGAATACTATGAAAAAGCTAAAAATCAAAATAAACTACGACCAGAAGACATAGAAAA
AATAGTTACAACATACAAAAATAGGGAAGAAATAGAAAAATACTCACATAAAGCAACAATTGAAGAAATTGAAGAAAATG
ATTACAACCTAAACATACCAAGATACGTTGACACGTTCGAGGAAGAAGAACCAATAGATTTAGATGAAGTAGTACGTGAA
ATACGTAAAATAGATGAAGAAATGAAAGAAGTAGATGCAAAAATCATAAAATATTGCAAAGAATTAGGAATAGAACCACC
AATATAA

Upstream 100 bases:

>100_bases
ATGGAATTATTAAATAAAAGAACCTTATTAAATAGGAGAAAAATAGAAATAGAAGAAGAATATCTTCATGGAATAATACA
AAAAGAATTAGGTGGTCAAT

Downstream 100 bases:

>100_bases
AGGGGATTATATGTGTAAAATTACAAAAGAAAATGACTCTTTGCAAAGACAAACAGAAAATAAAGATGAAGATAGTCAGG
AATTGCAATGTAATAAAAAC

Product: putative type I restriction-modification system, methyltransferase subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSNYETKLWAIADKLRGNMDANEFKNYMLGFIFYRYLSEKLEMTLNELLEEDGINFQEAYQDEELIEDLKEEGIEKLGYF
IQPKYLFSSVINEIDKGREILECLSNAFIEINDSSFNTESQDDFQNLFEDVDLNSSKLGNTNAEKNKLISGILQDISDID
FELEKDNSDILGDAYEYLISQFASSAGKKAGEFYTPQEVSTILARIVTLNKTRLKSVYDPTCGSGSLLLRVSKEADVSEF
YGQELNQTTYNLARMNMILHGVKYNHFNIKQGDSLENDRHEELKFDAVVANPPFSAKWSSDKSFINDERFSGYGKLAPKS
KADYAFVQHMIYHLNEQGTLAVVLPHGVLFRGAAEGTIRKYLIKELNYLDAVIGLPKNIFYGTSIPTCILVFKKCREEDD
NILFIDASEYYEKAKNQNKLRPEDIEKIVTTYKNREEIEKYSHKATIEEIEENDYNLNIPRYVDTFEEEEPIDLDEVVRE
IRKIDEEMKEVDAKIIKYCKELGIEPPI

Sequences:

>Translated_508_residues
MSNYETKLWAIADKLRGNMDANEFKNYMLGFIFYRYLSEKLEMTLNELLEEDGINFQEAYQDEELIEDLKEEGIEKLGYF
IQPKYLFSSVINEIDKGREILECLSNAFIEINDSSFNTESQDDFQNLFEDVDLNSSKLGNTNAEKNKLISGILQDISDID
FELEKDNSDILGDAYEYLISQFASSAGKKAGEFYTPQEVSTILARIVTLNKTRLKSVYDPTCGSGSLLLRVSKEADVSEF
YGQELNQTTYNLARMNMILHGVKYNHFNIKQGDSLENDRHEELKFDAVVANPPFSAKWSSDKSFINDERFSGYGKLAPKS
KADYAFVQHMIYHLNEQGTLAVVLPHGVLFRGAAEGTIRKYLIKELNYLDAVIGLPKNIFYGTSIPTCILVFKKCREEDD
NILFIDASEYYEKAKNQNKLRPEDIEKIVTTYKNREEIEKYSHKATIEEIEENDYNLNIPRYVDTFEEEEPIDLDEVVRE
IRKIDEEMKEVDAKIIKYCKELGIEPPI
>Mature_507_residues
SNYETKLWAIADKLRGNMDANEFKNYMLGFIFYRYLSEKLEMTLNELLEEDGINFQEAYQDEELIEDLKEEGIEKLGYFI
QPKYLFSSVINEIDKGREILECLSNAFIEINDSSFNTESQDDFQNLFEDVDLNSSKLGNTNAEKNKLISGILQDISDIDF
ELEKDNSDILGDAYEYLISQFASSAGKKAGEFYTPQEVSTILARIVTLNKTRLKSVYDPTCGSGSLLLRVSKEADVSEFY
GQELNQTTYNLARMNMILHGVKYNHFNIKQGDSLENDRHEELKFDAVVANPPFSAKWSSDKSFINDERFSGYGKLAPKSK
ADYAFVQHMIYHLNEQGTLAVVLPHGVLFRGAAEGTIRKYLIKELNYLDAVIGLPKNIFYGTSIPTCILVFKKCREEDDN
ILFIDASEYYEKAKNQNKLRPEDIEKIVTTYKNREEIEKYSHKATIEEIEENDYNLNIPRYVDTFEEEEPIDLDEVVREI
RKIDEEMKEVDAKIIKYCKELGIEPPI

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=281, Percent_Identity=27.4021352313167, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58622; Mature: 58491

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYETKLWAIADKLRGNMDANEFKNYMLGFIFYRYLSEKLEMTLNELLEEDGINFQEAY
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QDEELIEDLKEEGIEKLGYFIQPKYLFSSVINEIDKGREILECLSNAFIEINDSSFNTES
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCC
QDDFQNLFEDVDLNSSKLGNTNAEKNKLISGILQDISDIDFELEKDNSDILGDAYEYLIS
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
QFASSAGKKAGEFYTPQEVSTILARIVTLNKTRLKSVYDPTCGSGSLLLRVSKEADVSEF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
YGQELNQTTYNLARMNMILHGVKYNHFNIKQGDSLENDRHEELKFDAVVANPPFSAKWSS
HHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCC
DKSFINDERFSGYGKLAPKSKADYAFVQHMIYHLNEQGTLAVVLPHGVLFRGAAEGTIRK
CCHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHH
YLIKELNYLDAVIGLPKNIFYGTSIPTCILVFKKCREEDDNILFIDASEYYEKAKNQNKL
HHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCC
RPEDIEKIVTTYKNREEIEKYSHKATIEEIEENDYNLNIPRYVDTFEEEEPIDLDEVVRE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHH
IRKIDEEMKEVDAKIIKYCKELGIEPPI
HHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNYETKLWAIADKLRGNMDANEFKNYMLGFIFYRYLSEKLEMTLNELLEEDGINFQEAY
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QDEELIEDLKEEGIEKLGYFIQPKYLFSSVINEIDKGREILECLSNAFIEINDSSFNTES
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCC
QDDFQNLFEDVDLNSSKLGNTNAEKNKLISGILQDISDIDFELEKDNSDILGDAYEYLIS
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
QFASSAGKKAGEFYTPQEVSTILARIVTLNKTRLKSVYDPTCGSGSLLLRVSKEADVSEF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
YGQELNQTTYNLARMNMILHGVKYNHFNIKQGDSLENDRHEELKFDAVVANPPFSAKWSS
HHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCC
DKSFINDERFSGYGKLAPKSKADYAFVQHMIYHLNEQGTLAVVLPHGVLFRGAAEGTIRK
CCHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHH
YLIKELNYLDAVIGLPKNIFYGTSIPTCILVFKKCREEDDNILFIDASEYYEKAKNQNKL
HHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCC
RPEDIEKIVTTYKNREEIEKYSHKATIEEIEENDYNLNIPRYVDTFEEEEPIDLDEVVRE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHH
IRKIDEEMKEVDAKIIKYCKELGIEPPI
HHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]