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The map label for this gene is guaB

Identifier: 84489243

GI number: 84489243

Start: 498754

End: 500250

Strand: Direct

Name: guaB

Synonym: Msp_0431

Alternate gene names: 84489243

Gene position: 498754-500250 (Clockwise)

Preceding gene: 84489242

Following gene: 84489244

Centisome position: 28.22

GC content: 33.07

Gene sequence:

>1497_bases
ATGAATAAATATATGGATAAATTAACAAAAGCAGAAGACACATATACATTTGATGATTTTCTTATAAAACCAGGATTATC
ATGTATAGAACCAAATGATGTAAAATTAGATACACAAGTATCTACAAACTATCATTTAAACACACCAGTTGTAAGTTCGG
CTATGGATACTGTTACAGAAGCAAACATGGCAATATCTCTAGCAAGACAAGGTGGACTTGGTGTTATTCATAGAAATTTA
ACTATTGAACAAGAAATTAAAGAAATTAAAAAGGTTAAATATGCTAATGAATTAACAGTTAAAGAAGTTATATCAATATC
TCCTGATGAAACAGTTTCTGAAGCACAACAAATCATGGATATTGAAGAAATAAGTGGTTTACCCGTAGTAAATGATGATA
ATATTGTTGTAGGTATTATAAGTCGTAGAGATATTAAACCTTTAAGAGGTAAATACTTAAATCGTAAAGTATCCGATGCA
ATGACTCAGGAAGTAGTTACAATCTCTGAAAACACAACTACTGAAGAAGCATTAGATGTTGCATATGAAAATAAAGTTGA
AAGGTTACCAGTAGTTTCTGATGTTAATGAACTTCTTGGTATAGTAACAATGAAAGATATTTTAGAACGTAAAAAATATC
CTGATGCAGCACGTGATAAAAATGGTAGATATCTTGTTGCAGCAGCATGTGGTCCGTTTGATATTGAAAGAGCAATGGCA
ATAGATGATGCTGGGGCAGATATTATTGCTATAGATAGTGCACATGGACATAAAACTGATATTTTCAAATCTGTTAATGA
AATGAAGAAAAATGTTGATGCAGATATATTACTTGGTAATATTGCAACTGCAAAGGCTGCAAAAGACATGTTAGATGCAA
CAGAAATTGATGGTATAAAAGTTGGTATAGGTCCAGGTTCAATTTGTACCACTAGGATAGTTGCAGGTGTAGGTGTACCT
CAACTTAGTGCTGTTTCAAGTGTTGCAGATGTAGCAGAAGATCATGGAATACCTGTAATTGCTGATGGTGGTTTAAGATA
CTCTGGAGATATAGCAAAAGCATTAGCTGTTGGAGCAAATGCAGTTATGCTAGGTAGTTTACTTGCTGGAACAACTGAAT
CTCCTGGGGAAATGACTATTAGAAATGGACGTAAATATAAACAATATCGTGGAATGGGTTCTTTAGGTGCTATGACTGGT
GGAGTTGGAGCAGGAAAAGATAGGTATTTCCAAGGTTCAAGTAGTAATATGAATTCCTCAAAATTAGTACCAGAAGGTAT
TGAAGGAGTAGTTCCATATAAAGGAGAAGCTAGTCAAATAGTTTATCAATTAATGGGTGGTTTAAAATCATCAATGGGTT
ATGTTGGGGCTAAATCAATTAAAGAAATGCATGAAAAAGCAGAATTAGTTCATATAACTCCTAATGGTATGTCTGAAAGT
CATCCTCATGATATTACCATTACTAATGAAAGTCCAAACTATCATCCAAGATATTAA

Upstream 100 bases:

>100_bases
AGATAAAAATAATACTAATAGAAATACTAATAATATTTTTTTTAAATTTTTTTAGTATATGACTTTTGAATGATTATTTA
TTAAATAAAAGGGGCGTTAT

Downstream 100 bases:

>100_bases
ATAAAAATAAATCTAAGCTCCTTTTATTATTTTTTTTTAAATTATTTGAGAATGTGTAAGAATTAATTTTTAATTAATAT
TTTTTTTCTTATTTTAGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: IMP dehydrogenase; IMPD; IMPDH [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNKYMDKLTKAEDTYTFDDFLIKPGLSCIEPNDVKLDTQVSTNYHLNTPVVSSAMDTVTEANMAISLARQGGLGVIHRNL
TIEQEIKEIKKVKYANELTVKEVISISPDETVSEAQQIMDIEEISGLPVVNDDNIVVGIISRRDIKPLRGKYLNRKVSDA
MTQEVVTISENTTTEEALDVAYENKVERLPVVSDVNELLGIVTMKDILERKKYPDAARDKNGRYLVAAACGPFDIERAMA
IDDAGADIIAIDSAHGHKTDIFKSVNEMKKNVDADILLGNIATAKAAKDMLDATEIDGIKVGIGPGSICTTRIVAGVGVP
QLSAVSSVADVAEDHGIPVIADGGLRYSGDIAKALAVGANAVMLGSLLAGTTESPGEMTIRNGRKYKQYRGMGSLGAMTG
GVGAGKDRYFQGSSSNMNSSKLVPEGIEGVVPYKGEASQIVYQLMGGLKSSMGYVGAKSIKEMHEKAELVHITPNGMSES
HPHDITITNESPNYHPRY

Sequences:

>Translated_498_residues
MNKYMDKLTKAEDTYTFDDFLIKPGLSCIEPNDVKLDTQVSTNYHLNTPVVSSAMDTVTEANMAISLARQGGLGVIHRNL
TIEQEIKEIKKVKYANELTVKEVISISPDETVSEAQQIMDIEEISGLPVVNDDNIVVGIISRRDIKPLRGKYLNRKVSDA
MTQEVVTISENTTTEEALDVAYENKVERLPVVSDVNELLGIVTMKDILERKKYPDAARDKNGRYLVAAACGPFDIERAMA
IDDAGADIIAIDSAHGHKTDIFKSVNEMKKNVDADILLGNIATAKAAKDMLDATEIDGIKVGIGPGSICTTRIVAGVGVP
QLSAVSSVADVAEDHGIPVIADGGLRYSGDIAKALAVGANAVMLGSLLAGTTESPGEMTIRNGRKYKQYRGMGSLGAMTG
GVGAGKDRYFQGSSSNMNSSKLVPEGIEGVVPYKGEASQIVYQLMGGLKSSMGYVGAKSIKEMHEKAELVHITPNGMSES
HPHDITITNESPNYHPRY
>Mature_498_residues
MNKYMDKLTKAEDTYTFDDFLIKPGLSCIEPNDVKLDTQVSTNYHLNTPVVSSAMDTVTEANMAISLARQGGLGVIHRNL
TIEQEIKEIKKVKYANELTVKEVISISPDETVSEAQQIMDIEEISGLPVVNDDNIVVGIISRRDIKPLRGKYLNRKVSDA
MTQEVVTISENTTTEEALDVAYENKVERLPVVSDVNELLGIVTMKDILERKKYPDAARDKNGRYLVAAACGPFDIERAMA
IDDAGADIIAIDSAHGHKTDIFKSVNEMKKNVDADILLGNIATAKAAKDMLDATEIDGIKVGIGPGSICTTRIVAGVGVP
QLSAVSSVADVAEDHGIPVIADGGLRYSGDIAKALAVGANAVMLGSLLAGTTESPGEMTIRNGRKYKQYRGMGSLGAMTG
GVGAGKDRYFQGSSSNMNSSKLVPEGIEGVVPYKGEASQIVYQLMGGLKSSMGYVGAKSIKEMHEKAELVHITPNGMSES
HPHDITITNESPNYHPRY

Specific function: IMP is the rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth [H]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

Organism=Homo sapiens, GI34328930, Length=462, Percent_Identity=37.2294372294372, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI217035146, Length=462, Percent_Identity=37.012987012987, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI156616279, Length=462, Percent_Identity=37.2294372294372, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI34328928, Length=462, Percent_Identity=37.2294372294372, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI66933016, Length=474, Percent_Identity=36.9198312236287, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI217035152, Length=464, Percent_Identity=37.2844827586207, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI217035148, Length=462, Percent_Identity=36.7965367965368, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI217035150, Length=474, Percent_Identity=34.5991561181435, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI50541954, Length=248, Percent_Identity=37.5, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI50541952, Length=248, Percent_Identity=37.5, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI50541948, Length=248, Percent_Identity=37.5, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI50541956, Length=248, Percent_Identity=37.5, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI156104880, Length=253, Percent_Identity=37.9446640316205, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI1788855, Length=493, Percent_Identity=46.8559837728195, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786293, Length=242, Percent_Identity=35.1239669421488, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71994385, Length=481, Percent_Identity=33.2640332640333, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71994389, Length=423, Percent_Identity=35.2245862884161, Blast_Score=223, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17560440, Length=226, Percent_Identity=36.283185840708, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323585, Length=478, Percent_Identity=37.0292887029289, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6323464, Length=470, Percent_Identity=37.2340425531915, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322012, Length=462, Percent_Identity=36.5800865800866, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319352, Length=337, Percent_Identity=34.7181008902077, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319353, Length=121, Percent_Identity=42.9752066115703, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24641071, Length=460, Percent_Identity=36.0869565217391, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24641073, Length=460, Percent_Identity=36.0869565217391, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI28571163, Length=421, Percent_Identity=36.104513064133, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR005990
- InterPro:   IPR018529
- InterPro:   IPR015875
- InterPro:   IPR001093 [H]

Pfam domain/function: PF00571 CBS; PF00478 IMPDH [H]

EC number: =1.1.1.205 [H]

Molecular weight: Translated: 53594; Mature: 53594

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYMDKLTKAEDTYTFDDFLIKPGLSCIEPNDVKLDTQVSTNYHLNTPVVSSAMDTVTE
CCHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHH
ANMAISLARQGGLGVIHRNLTIEQEIKEIKKVKYANELTVKEVISISPDETVSEAQQIMD
HHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
IEEISGLPVVNDDNIVVGIISRRDIKPLRGKYLNRKVSDAMTQEVVTISENTTTEEALDV
HHHHCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
AYENKVERLPVVSDVNELLGIVTMKDILERKKYPDAARDKNGRYLVAAACGPFDIERAMA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCHHHHHE
IDDAGADIIAIDSAHGHKTDIFKSVNEMKKNVDADILLGNIATAKAAKDMLDATEIDGIK
ECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEE
VGIGPGSICTTRIVAGVGVPQLSAVSSVADVAEDHGIPVIADGGLRYSGDIAKALAVGAN
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHHHHHHCCC
AVMLGSLLAGTTESPGEMTIRNGRKYKQYRGMGSLGAMTGGVGAGKDRYFQGSSSNMNSS
HHHHHHHHHCCCCCCCCEEECCCCHHHHHCCCCCHHHCCCCCCCCCCCEECCCCCCCCCC
KLVPEGIEGVVPYKGEASQIVYQLMGGLKSSMGYVGAKSIKEMHEKAELVHITPNGMSES
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCC
HPHDITITNESPNYHPRY
CCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MNKYMDKLTKAEDTYTFDDFLIKPGLSCIEPNDVKLDTQVSTNYHLNTPVVSSAMDTVTE
CCHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHH
ANMAISLARQGGLGVIHRNLTIEQEIKEIKKVKYANELTVKEVISISPDETVSEAQQIMD
HHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
IEEISGLPVVNDDNIVVGIISRRDIKPLRGKYLNRKVSDAMTQEVVTISENTTTEEALDV
HHHHCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
AYENKVERLPVVSDVNELLGIVTMKDILERKKYPDAARDKNGRYLVAAACGPFDIERAMA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCHHHHHE
IDDAGADIIAIDSAHGHKTDIFKSVNEMKKNVDADILLGNIATAKAAKDMLDATEIDGIK
ECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEE
VGIGPGSICTTRIVAGVGVPQLSAVSSVADVAEDHGIPVIADGGLRYSGDIAKALAVGAN
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHHHHHHCCC
AVMLGSLLAGTTESPGEMTIRNGRKYKQYRGMGSLGAMTGGVGAGKDRYFQGSSSNMNSS
HHHHHHHHHCCCCCCCCEEECCCCHHHHHCCCCCHHHCCCCCCCCCCCEECCCCCCCCCC
KLVPEGIEGVVPYKGEASQIVYQLMGGLKSSMGYVGAKSIKEMHEKAELVHITPNGMSES
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCC
HPHDITITNESPNYHPRY
CCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]