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The map label for this gene is cofH

Identifier: 84489204

GI number: 84489204

Start: 454718

End: 455767

Strand: Direct

Name: cofH

Synonym: Msp_0392

Alternate gene names: 84489204

Gene position: 454718-455767 (Clockwise)

Preceding gene: 84489203

Following gene: 84489206

Centisome position: 25.73

GC content: 30.1

Gene sequence:

>1050_bases
GTGATAGAAGAAATATATAATAAATCATTAGATAATGAAATTACTAAAGAAGATGCTATAGAATTAGTCAGATCTGCAAA
TCAGTTTGAATTATTTGATACTGCTGATAAATTAAGACAAACTATTGTTGGTGATAAAGTAACTTATGTTGTTAATAAGG
CTATTGATATAACTGATAATTGTATGATTGGCTGTAAATTCTGTTCTTTTAGAAATTTTGAAAATTATAAAATGACTAAT
GAAGAAATTTCTGAAAGTATTGTTCAGGCAAAAACAGTGGGAGCTACTGAGATATGTTTATTTGGCGGAATTACTAGGGA
TATGGATATAGATTATTATTGTAATCTTATCGAAAATATTAAAAATCAACATGAAATATGTTTACATGCATTATCTCCTG
CAGAAATATATCAAACTGCTAAAAATTCAAATATAACTACTTTTGAAGCTTTAAGTAGGTTAAAAGATGTAGGAATGGAT
ACAATGACTGGTGCATCTGCAGAAATTTTAGTTGATTCAATAAGAAAACAAATCTGTCCAAATAAGGTATCTACAGCCGA
TTGGGCAAAGATTGTTAAGGAAGCTCATGAATTGGGAATTCCAACAACTTCAACTATAATGTATGGAAGTATTGAAACAT
GGGAAGATAGGATAGAACACATGTTTTTATTAAAGGAAATTCAGGAAGAAACTCATGGATTTACTGAATTTGTTCCAATG
ACATTTTTAGGTGAAAATAATGAGCTTGGAAAAATATCTAATGGTGCTACAGGTATTGAGGATTTAAAAATCCATGCAAT
TTCCAGAATAATCTTTGGGGATGTAATTCCAAATATTCAAGTTTCATGGGTAAAGTTAGGCCTAAGAATGACACAAGTTG
CTCTAACATGTGGTGCTAATGATATTGGTGGAACAATGATTGAAGATAAAATATCCACAGCTGCAGGTGGTGGTTATGGT
GGATATTTACCTGTAGAAAAAATCAAACAATTAATTAAGGATATTGGTAGAATTCCACAAGAGAGAACTACTAAGTATGA
AATGTTGTAA

Upstream 100 bases:

>100_bases
TATTAAATAAATTATTACTTAATATAATAATAGATTAAGAGAAGTTAATGTGTTATAAGAATTTTTGTATTTATTTTTTA
TATTCATTAAGGTGGATTTT

Downstream 100 bases:

>100_bases
TTGTTTTTAAGAAGAAGTATGTATTAATATGATATGTATTTCAACACTATTTTTTTTTATTTTATTGAATTATTCTCTAT
TTTATAATAAATATAAAAAA

Product: CofH

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIEEIYNKSLDNEITKEDAIELVRSANQFELFDTADKLRQTIVGDKVTYVVNKAIDITDNCMIGCKFCSFRNFENYKMTN
EEISESIVQAKTVGATEICLFGGITRDMDIDYYCNLIENIKNQHEICLHALSPAEIYQTAKNSNITTFEALSRLKDVGMD
TMTGASAEILVDSIRKQICPNKVSTADWAKIVKEAHELGIPTTSTIMYGSIETWEDRIEHMFLLKEIQEETHGFTEFVPM
TFLGENNELGKISNGATGIEDLKIHAISRIIFGDVIPNIQVSWVKLGLRMTQVALTCGANDIGGTMIEDKISTAAGGGYG
GYLPVEKIKQLIKDIGRIPQERTTKYEML

Sequences:

>Translated_349_residues
MIEEIYNKSLDNEITKEDAIELVRSANQFELFDTADKLRQTIVGDKVTYVVNKAIDITDNCMIGCKFCSFRNFENYKMTN
EEISESIVQAKTVGATEICLFGGITRDMDIDYYCNLIENIKNQHEICLHALSPAEIYQTAKNSNITTFEALSRLKDVGMD
TMTGASAEILVDSIRKQICPNKVSTADWAKIVKEAHELGIPTTSTIMYGSIETWEDRIEHMFLLKEIQEETHGFTEFVPM
TFLGENNELGKISNGATGIEDLKIHAISRIIFGDVIPNIQVSWVKLGLRMTQVALTCGANDIGGTMIEDKISTAAGGGYG
GYLPVEKIKQLIKDIGRIPQERTTKYEML
>Mature_349_residues
MIEEIYNKSLDNEITKEDAIELVRSANQFELFDTADKLRQTIVGDKVTYVVNKAIDITDNCMIGCKFCSFRNFENYKMTN
EEISESIVQAKTVGATEICLFGGITRDMDIDYYCNLIENIKNQHEICLHALSPAEIYQTAKNSNITTFEALSRLKDVGMD
TMTGASAEILVDSIRKQICPNKVSTADWAKIVKEAHELGIPTTSTIMYGSIETWEDRIEHMFLLKEIQEETHGFTEFVPM
TFLGENNELGKISNGATGIEDLKIHAISRIIFGDVIPNIQVSWVKLGLRMTQVALTCGANDIGGTMIEDKISTAAGGGYG
GYLPVEKIKQLIKDIGRIPQERTTKYEML

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39054; Mature: 39054

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEIYNKSLDNEITKEDAIELVRSANQFELFDTADKLRQTIVGDKVTYVVNKAIDITDN
CCHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
CMIGCKFCSFRNFENYKMTNEEISESIVQAKTVGATEICLFGGITRDMDIDYYCNLIENI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHH
KNQHEICLHALSPAEIYQTAKNSNITTFEALSRLKDVGMDTMTGASAEILVDSIRKQICP
CCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NKVSTADWAKIVKEAHELGIPTTSTIMYGSIETWEDRIEHMFLLKEIQEETHGFTEFVPM
CCCCHHHHHHHHHHHHHCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TFLGENNELGKISNGATGIEDLKIHAISRIIFGDVIPNIQVSWVKLGLRMTQVALTCGAN
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCC
DIGGTMIEDKISTAAGGGYGGYLPVEKIKQLIKDIGRIPQERTTKYEML
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MIEEIYNKSLDNEITKEDAIELVRSANQFELFDTADKLRQTIVGDKVTYVVNKAIDITDN
CCHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
CMIGCKFCSFRNFENYKMTNEEISESIVQAKTVGATEICLFGGITRDMDIDYYCNLIENI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHH
KNQHEICLHALSPAEIYQTAKNSNITTFEALSRLKDVGMDTMTGASAEILVDSIRKQICP
CCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NKVSTADWAKIVKEAHELGIPTTSTIMYGSIETWEDRIEHMFLLKEIQEETHGFTEFVPM
CCCCHHHHHHHHHHHHHCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TFLGENNELGKISNGATGIEDLKIHAISRIIFGDVIPNIQVSWVKLGLRMTQVALTCGAN
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCC
DIGGTMIEDKISTAAGGGYGGYLPVEKIKQLIKDIGRIPQERTTKYEML
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA