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The map label for this gene is glnA

Identifier: 84489061

GI number: 84489061

Start: 291990

End: 293327

Strand: Direct

Name: glnA

Synonym: Msp_0234

Alternate gene names: 84489061

Gene position: 291990-293327 (Clockwise)

Preceding gene: 84489060

Following gene: 84489062

Centisome position: 16.52

GC content: 34.75

Gene sequence:

>1338_bases
ATGTCCGCACGAGATGACAAAATAGATGAAATTGTTAAAACAGTAGATGAACATGGTTCAAAATTTTTAAGGTTCCAATT
TGTAGATATACATGGATTACCAAAAAATGTAGCAGTATCTTTAAATAAACCAGAAGATGTAGAAACAATTATTGAAGATG
GATTATTATTTGATGGATCTTCAGTAGCAGGATTTGTAGGAATCAATGATAGTGACCTTGCATTAAAACCAGATATAAAT
ACATTCTCAACTCTACCATGGAGACCAGATGAATATGGTGCATGTAGATTCATATGTGATGTATACCACACAGATGGAAC
ACCATTTGAAGGAGACCCTAGAGGAGTTCTTAAAAAATCATTAAAATTAGCAAAAGATAGAGGTTACCAATACAACATGG
GTCCAGAACCAGAATTTTTCCTTGTTGACCATGATGAAAATGGAAAACTTATTCCAGCAGATAATGCAGAATACTTCGAT
GTAGAACCATTAGATCAAGGTATAGATGTAAGAAAAGAAATAGTATTAGGTCTTGAAAAACTAAACTTCAACATAGAAGT
AAGCCACCACGAAGTAGCACCAGGACAACACGAAGTTGACTTTAGATTTGATGATGCACTAAAAACAGCAGATGCTGTAG
TAACATTCAAACAAGCAATTAAAGCATTAGTAGACAACCTAGGATACCAAGTAACATTCATGCCAAAACCATTCTTTGGA
ATTAATGGTAGTGGAATGCACTGTAACCAAAGTCTATTTAAAGATGGTAAAAACATATTCTACGACCCAGATACAGAAAC
ACAACTATCCCAAGAAGCATTATACTTCATAGGAGGATTATTAAAACACGCAAAAGCATTATCTGCAATACTTTCCCCAA
CAATTAACTCATACAAACGTTTAGTACCAGGATATGAAGCACCATGTTACATAGCATACGGACTTAAAAATAGATCAACT
CTCCTAAGAATTCCAGCTTCTAGGGGATTAGGTACAAGAATTGAATGTAGATCTGCAGACCCATCATGTAACCCATACCT
TGCATTTGCAGTGTTACTTGAAGCAGGTCTCGATGGATTAGACAATAAAATTGACCCAGGAGAACCAACAGAATTCAATG
CATTTGCTCTTACTCCTGATGAATTAGCAGCAAAAGGTATTGATACATTACCTACAAGTCTATGGGAAGCATATCATTCC
TTAGAAAAAGACGATGTAGTTAAAAATGCTCTTGGAGATTATGTATACGATCAATTCTATAACATTAAACGTGCAGAATG
GGATGACTACAGAATACAAGTATTTGATTATGAACGTGATAAATTCCTTAACATCTAG

Upstream 100 bases:

>100_bases
TATTATACTATAAAAAAATAAATATAATATATATGAAATAACTTATTAACAAGTATCTTTCTTATTAAAACATTATAATA
TTGAATAGGTGAAAATTTTT

Downstream 100 bases:

>100_bases
ATATTATGTTATTATCTAATACTTTTCTCTCACCCTCTTTTTTTAAATATATTACATTGTACCATGTTTTTAATAATTAT
CAACAATAAATAATTATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSARDDKIDEIVKTVDEHGSKFLRFQFVDIHGLPKNVAVSLNKPEDVETIIEDGLLFDGSSVAGFVGINDSDLALKPDIN
TFSTLPWRPDEYGACRFICDVYHTDGTPFEGDPRGVLKKSLKLAKDRGYQYNMGPEPEFFLVDHDENGKLIPADNAEYFD
VEPLDQGIDVRKEIVLGLEKLNFNIEVSHHEVAPGQHEVDFRFDDALKTADAVVTFKQAIKALVDNLGYQVTFMPKPFFG
INGSGMHCNQSLFKDGKNIFYDPDTETQLSQEALYFIGGLLKHAKALSAILSPTINSYKRLVPGYEAPCYIAYGLKNRST
LLRIPASRGLGTRIECRSADPSCNPYLAFAVLLEAGLDGLDNKIDPGEPTEFNAFALTPDELAAKGIDTLPTSLWEAYHS
LEKDDVVKNALGDYVYDQFYNIKRAEWDDYRIQVFDYERDKFLNI

Sequences:

>Translated_445_residues
MSARDDKIDEIVKTVDEHGSKFLRFQFVDIHGLPKNVAVSLNKPEDVETIIEDGLLFDGSSVAGFVGINDSDLALKPDIN
TFSTLPWRPDEYGACRFICDVYHTDGTPFEGDPRGVLKKSLKLAKDRGYQYNMGPEPEFFLVDHDENGKLIPADNAEYFD
VEPLDQGIDVRKEIVLGLEKLNFNIEVSHHEVAPGQHEVDFRFDDALKTADAVVTFKQAIKALVDNLGYQVTFMPKPFFG
INGSGMHCNQSLFKDGKNIFYDPDTETQLSQEALYFIGGLLKHAKALSAILSPTINSYKRLVPGYEAPCYIAYGLKNRST
LLRIPASRGLGTRIECRSADPSCNPYLAFAVLLEAGLDGLDNKIDPGEPTEFNAFALTPDELAAKGIDTLPTSLWEAYHS
LEKDDVVKNALGDYVYDQFYNIKRAEWDDYRIQVFDYERDKFLNI
>Mature_444_residues
SARDDKIDEIVKTVDEHGSKFLRFQFVDIHGLPKNVAVSLNKPEDVETIIEDGLLFDGSSVAGFVGINDSDLALKPDINT
FSTLPWRPDEYGACRFICDVYHTDGTPFEGDPRGVLKKSLKLAKDRGYQYNMGPEPEFFLVDHDENGKLIPADNAEYFDV
EPLDQGIDVRKEIVLGLEKLNFNIEVSHHEVAPGQHEVDFRFDDALKTADAVVTFKQAIKALVDNLGYQVTFMPKPFFGI
NGSGMHCNQSLFKDGKNIFYDPDTETQLSQEALYFIGGLLKHAKALSAILSPTINSYKRLVPGYEAPCYIAYGLKNRSTL
LRIPASRGLGTRIECRSADPSCNPYLAFAVLLEAGLDGLDNKIDPGEPTEFNAFALTPDELAAKGIDTLPTSLWEAYHSL
EKDDVVKNALGDYVYDQFYNIKRAEWDDYRIQVFDYERDKFLNI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=461, Percent_Identity=25.3796095444685, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1790301, Length=461, Percent_Identity=39.6963123644252, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87081870, Length=286, Percent_Identity=30.0699300699301, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49927; Mature: 49795

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARDDKIDEIVKTVDEHGSKFLRFQFVDIHGLPKNVAVSLNKPEDVETIIEDGLLFDGS
CCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCEEECCC
SVAGFVGINDSDLALKPDINTFSTLPWRPDEYGACRFICDVYHTDGTPFEGDPRGVLKKS
CCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
LKLAKDRGYQYNMGPEPEFFLVDHDENGKLIPADNAEYFDVEPLDQGIDVRKEIVLGLEK
HHHHHHCCEEECCCCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LNFNIEVSHHEVAPGQHEVDFRFDDALKTADAVVTFKQAIKALVDNLGYQVTFMPKPFFG
CCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
INGSGMHCNQSLFKDGKNIFYDPDTETQLSQEALYFIGGLLKHAKALSAILSPTINSYKR
CCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGYEAPCYIAYGLKNRSTLLRIPASRGLGTRIECRSADPSCNPYLAFAVLLEAGLDGL
HCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCC
DNKIDPGEPTEFNAFALTPDELAAKGIDTLPTSLWEAYHSLEKDDVVKNALGDYVYDQFY
CCCCCCCCCCCCEEEEECHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NIKRAEWDDYRIQVFDYERDKFLNI
CCCCCCCCCEEEEEEECCCHHCCCC
>Mature Secondary Structure 
SARDDKIDEIVKTVDEHGSKFLRFQFVDIHGLPKNVAVSLNKPEDVETIIEDGLLFDGS
CCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCEEECCC
SVAGFVGINDSDLALKPDINTFSTLPWRPDEYGACRFICDVYHTDGTPFEGDPRGVLKKS
CCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
LKLAKDRGYQYNMGPEPEFFLVDHDENGKLIPADNAEYFDVEPLDQGIDVRKEIVLGLEK
HHHHHHCCEEECCCCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LNFNIEVSHHEVAPGQHEVDFRFDDALKTADAVVTFKQAIKALVDNLGYQVTFMPKPFFG
CCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
INGSGMHCNQSLFKDGKNIFYDPDTETQLSQEALYFIGGLLKHAKALSAILSPTINSYKR
CCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGYEAPCYIAYGLKNRSTLLRIPASRGLGTRIECRSADPSCNPYLAFAVLLEAGLDGL
HCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCC
DNKIDPGEPTEFNAFALTPDELAAKGIDTLPTSLWEAYHSLEKDDVVKNALGDYVYDQFY
CCCCCCCCCCCCEEEEECHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NIKRAEWDDYRIQVFDYERDKFLNI
CCCCCCCCCEEEEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]