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The map label for this gene is thiC1

Identifier: 84489056

GI number: 84489056

Start: 286378

End: 287667

Strand: Direct

Name: thiC1

Synonym: Msp_0228

Alternate gene names: 84489056

Gene position: 286378-287667 (Clockwise)

Preceding gene: 84489055

Following gene: 84489057

Centisome position: 16.2

GC content: 32.95

Gene sequence:

>1290_bases
TTGACTCAATTAGAATATGCAAGAAAAGGAGAAATAACTCCTGAAATGGAATATGTAGCAAAAACTGAAAATATAGATGT
TGAAAAATTAAGAAAAAATATAGCTTCTGGAAAAGTAGTTATACCAAAAAATGTGCATAATAATACTCTTCCAACTGGAA
TTGGTAAAGATTTACATACAAAAATTAATGCAAATATAGGATCATCTACAGAAATGGAAGATATTAATGTAGAGCTAGAA
AAACTAGATATACTACTAAAATATGGTGCAGATGCTGTGATGGATTTAAGTACTGGACCAAAATTACATGAAATCAGAAA
GGCAATAAGAGACAAAACAAATATACCTCTAGGAACAGTTCCAATATATGAAGCAGGAGTAGAAACAACTTCTCAAGATA
AAATTATTGTAGATATGGATGATGATATTATCTTTAAAACAATAATAAATCAAGCAAAAGAAGGTGTTGACTTTATAACA
GTACATTGTGGGATTACACAAGATGCAATAAAAGCAGTTGATGATTCAGAAAGATTAATGGGAATAGTAAGTAGGGGTGG
AGCTCTAACAGCTGCATGGATCATGCATAATGAAAGAGAAAATCCATTATATGCAGAATATGATTATTTACTAGAAATAT
GTAAAGAACATGATGTAACACTCTCTCTAGGTGATGGTTTAAGACCAGGTTGTATTCATGATGCAACTGATATTGCACAG
ATAAGAGAATTAACAACACTAGGAAGACTTGTAAAACGTTCACAAAAAGCTGGAGTACAAGTGATGGTTGAAGGACCAGG
TCATGTTCCTATAACACAAGTAAAGGCAAATATGCAAATACAAAAAACCATATGTTCAGATGCACCATTCTATGTTTTAG
GACCTTTAGTAACAGATGTTGCACCAGGTTATGATCATATTACAGCAGCAATAGGTGCAAGTATTGCAGGTGCAAGTGGG
GCTGACTTTTTATGTTATGTTACACCTGCAGAACATCTGTGTATACCAAATAAGGAACATGTAAAACAGGGTGTTATAGC
TTCAAAGATTGCTGCAGAAGTATCTGATATAGCCAAACAAATACCAAGTACAATGAAAAGAGAATATGATATGGCTGTTG
CAAGGGATAACTTTGACTGGGAAAAACAATTTGAATTGGCAATTGATGGAGAAACTGCACGTAAGTATTATGAAAGTACA
TCTACATCTGATGAGGAGATGTGTAGTATGTGTGGAGATTTCTGTGCTATAAAAATGGTTAAAGATCATGAAAAAGCAAA
TAAGAATTAG

Upstream 100 bases:

>100_bases
AAGTACAAATAAATATCCACAGGAAAATAACAATATACCTAATCCATTAGTATAAAAAAAGATTTTGTTTATAAGAAAAA
ATTATAGAGGAGAGTATTAA

Downstream 100 bases:

>100_bases
GTATTAATCTTATTTTTGGAAGTTAAAGTAGTATATTTAAAAAATATACGTAATTATGTGTTAAATTGTATTTTATATGG
ATAATTATTAACTTCCATAC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTQLEYARKGEITPEMEYVAKTENIDVEKLRKNIASGKVVIPKNVHNNTLPTGIGKDLHTKINANIGSSTEMEDINVELE
KLDILLKYGADAVMDLSTGPKLHEIRKAIRDKTNIPLGTVPIYEAGVETTSQDKIIVDMDDDIIFKTIINQAKEGVDFIT
VHCGITQDAIKAVDDSERLMGIVSRGGALTAAWIMHNERENPLYAEYDYLLEICKEHDVTLSLGDGLRPGCIHDATDIAQ
IRELTTLGRLVKRSQKAGVQVMVEGPGHVPITQVKANMQIQKTICSDAPFYVLGPLVTDVAPGYDHITAAIGASIAGASG
ADFLCYVTPAEHLCIPNKEHVKQGVIASKIAAEVSDIAKQIPSTMKREYDMAVARDNFDWEKQFELAIDGETARKYYEST
STSDEEMCSMCGDFCAIKMVKDHEKANKN

Sequences:

>Translated_429_residues
MTQLEYARKGEITPEMEYVAKTENIDVEKLRKNIASGKVVIPKNVHNNTLPTGIGKDLHTKINANIGSSTEMEDINVELE
KLDILLKYGADAVMDLSTGPKLHEIRKAIRDKTNIPLGTVPIYEAGVETTSQDKIIVDMDDDIIFKTIINQAKEGVDFIT
VHCGITQDAIKAVDDSERLMGIVSRGGALTAAWIMHNERENPLYAEYDYLLEICKEHDVTLSLGDGLRPGCIHDATDIAQ
IRELTTLGRLVKRSQKAGVQVMVEGPGHVPITQVKANMQIQKTICSDAPFYVLGPLVTDVAPGYDHITAAIGASIAGASG
ADFLCYVTPAEHLCIPNKEHVKQGVIASKIAAEVSDIAKQIPSTMKREYDMAVARDNFDWEKQFELAIDGETARKYYEST
STSDEEMCSMCGDFCAIKMVKDHEKANKN
>Mature_428_residues
TQLEYARKGEITPEMEYVAKTENIDVEKLRKNIASGKVVIPKNVHNNTLPTGIGKDLHTKINANIGSSTEMEDINVELEK
LDILLKYGADAVMDLSTGPKLHEIRKAIRDKTNIPLGTVPIYEAGVETTSQDKIIVDMDDDIIFKTIINQAKEGVDFITV
HCGITQDAIKAVDDSERLMGIVSRGGALTAAWIMHNERENPLYAEYDYLLEICKEHDVTLSLGDGLRPGCIHDATDIAQI
RELTTLGRLVKRSQKAGVQVMVEGPGHVPITQVKANMQIQKTICSDAPFYVLGPLVTDVAPGYDHITAAIGASIAGASGA
DFLCYVTPAEHLCIPNKEHVKQGVIASKIAAEVSDIAKQIPSTMKREYDMAVARDNFDWEKQFELAIDGETARKYYESTS
TSDEEMCSMCGDFCAIKMVKDHEKANKN

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=455, Percent_Identity=44.3956043956044, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC1_METST (Q2NHI8)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447288.1
- STRING:   Q2NHI8
- GeneID:   3854774
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0228
- NMPDR:   fig|339860.6.peg.225
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- PhylomeDB:   Q2NHI8
- ProtClustDB:   PRK13352
- BioCyc:   MSTA339860:MSP_0228-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47198; Mature: 47067

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEYARKGEITPEMEYVAKTENIDVEKLRKNIASGKVVIPKNVHNNTLPTGIGKDLHT
CCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHH
KINANIGSSTEMEDINVELEKLDILLKYGADAVMDLSTGPKLHEIRKAIRDKTNIPLGTV
EEECCCCCCCCCHHCCEEHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC
PIYEAGVETTSQDKIIVDMDDDIIFKTIINQAKEGVDFITVHCGITQDAIKAVDDSERLM
CHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHH
GIVSRGGALTAAWIMHNERENPLYAEYDYLLEICKEHDVTLSLGDGLRPGCIHDATDIAQ
HHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHH
IRELTTLGRLVKRSQKAGVQVMVEGPGHVPITQVKANMQIQKTICSDAPFYVLGPLVTDV
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCHHHHHHCCCCCEEEHHHHHHC
APGYDHITAAIGASIAGASGADFLCYVTPAEHLCIPNKEHVKQGVIASKIAAEVSDIAKQ
CCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IPSTMKREYDMAVARDNFDWEKQFELAIDGETARKYYESTSTSDEEMCSMCGDFCAIKMV
HHHHHHHHHHHHEECCCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KDHEKANKN
HHHHHCCCC
>Mature Secondary Structure 
TQLEYARKGEITPEMEYVAKTENIDVEKLRKNIASGKVVIPKNVHNNTLPTGIGKDLHT
CCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHH
KINANIGSSTEMEDINVELEKLDILLKYGADAVMDLSTGPKLHEIRKAIRDKTNIPLGTV
EEECCCCCCCCCHHCCEEHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC
PIYEAGVETTSQDKIIVDMDDDIIFKTIINQAKEGVDFITVHCGITQDAIKAVDDSERLM
CHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHH
GIVSRGGALTAAWIMHNERENPLYAEYDYLLEICKEHDVTLSLGDGLRPGCIHDATDIAQ
HHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHH
IRELTTLGRLVKRSQKAGVQVMVEGPGHVPITQVKANMQIQKTICSDAPFYVLGPLVTDV
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCHHHHHHCCCCCEEEHHHHHHC
APGYDHITAAIGASIAGASGADFLCYVTPAEHLCIPNKEHVKQGVIASKIAAEVSDIAKQ
CCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IPSTMKREYDMAVARDNFDWEKQFELAIDGETARKYYESTSTSDEEMCSMCGDFCAIKMV
HHHHHHHHHHHHEECCCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KDHEKANKN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA