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The map label for this gene is mtaC2

Identifier: 84489015

GI number: 84489015

Start: 231535

End: 232374

Strand: Direct

Name: mtaC2

Synonym: Msp_0187

Alternate gene names: 84489015

Gene position: 231535-232374 (Clockwise)

Preceding gene: 84489014

Following gene: 84489016

Centisome position: 13.1

GC content: 36.55

Gene sequence:

>840_bases
ATGTCTGAAAGTCCATTAGCTAAATATAATGATTTAATATTTGATAATTATGAAGATATTGCAGTAAGATACAATGTTAA
AATTGAAGGTCCTGCTCTTAAACCTGAAGATGACCCAGAAGTAGTAGAAATATTACCTAAAGAAGAAGGAGTAAAAAGAT
CACTTGCTCTAACAGTATTATATGGTGACAAAGATGAATGTGATGCACAAGTAGAAAAAGCATTAGAAGCTGGCGAAGAT
CCAATAGACCTTATTAACAACGCTTTAATGAAAGGTATGGATGGTGTAAGTGCATTATATACTAAAGGTGAATTCTTCTT
ACCTGATTTAATGCTTGCAGGAGATGCAATGATGTCTGGAGTAGCAATTTGTGAAGATAAACTCGGACACAAATCAGAAA
CCAAAGCACCTGTAATGACCTTTGCTGTAGAAGGAGACCCTCATGACATTGGTAAAAACTTAATTGTAATGTTCTTAAAT
GCAAACGGATATGGTGCAATAGACCTTGGTCGTGACGTACCTACTGCTGAAGTAGTAAAACAAGCTCAAGAAAACAAACC
TGTTCTCATGACTGCTACTGCTTTAATGACAACAACAATGACTGAATTCAGTAAAATTGTAGCTGCACTTCAAGAAGCAG
GTATTGATACACCTATTGGATGTGGTGGAGGAGCTGTACGTCGTGACTTTGTAGAAGAAAGTCCACAAACTTTCTATGGT
GTAGAAGCATATCACGTACCTAAATTAGCAGATGCTATTGTAGATGATGGTAAAACTTGGGAAGACATCAGAAACGAATA
CTCTGACATTGTTGGAGAATACGTAGCAGCATACTCCTAA

Upstream 100 bases:

>100_bases
ATTTGCAAAAGCTTGTATAAAACGTTACGGAAGAAAAGTAAAAGAACACGATCCATCACAATATGAATTATAAGTGTTAT
ATATAATAGGAAGTGAAATT

Downstream 100 bases:

>100_bases
GTATACTTAGAAAATCTTTCACAGAGTAATTATTTTATTGATTATTCGGGATTATGATAAGAAATTTACTTTCTTATCTT
TTTTTTATTATTATTTTTAG

Product: MtaC2

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 1 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSESPLAKYNDLIFDNYEDIAVRYNVKIEGPALKPEDDPEVVEILPKEEGVKRSLALTVLYGDKDECDAQVEKALEAGED
PIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVAICEDKLGHKSETKAPVMTFAVEGDPHDIGKNLIVMFLN
ANGYGAIDLGRDVPTAEVVKQAQENKPVLMTATALMTTTMTEFSKIVAALQEAGIDTPIGCGGGAVRRDFVEESPQTFYG
VEAYHVPKLADAIVDDGKTWEDIRNEYSDIVGEYVAAYS

Sequences:

>Translated_279_residues
MSESPLAKYNDLIFDNYEDIAVRYNVKIEGPALKPEDDPEVVEILPKEEGVKRSLALTVLYGDKDECDAQVEKALEAGED
PIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVAICEDKLGHKSETKAPVMTFAVEGDPHDIGKNLIVMFLN
ANGYGAIDLGRDVPTAEVVKQAQENKPVLMTATALMTTTMTEFSKIVAALQEAGIDTPIGCGGGAVRRDFVEESPQTFYG
VEAYHVPKLADAIVDDGKTWEDIRNEYSDIVGEYVAAYS
>Mature_278_residues
SESPLAKYNDLIFDNYEDIAVRYNVKIEGPALKPEDDPEVVEILPKEEGVKRSLALTVLYGDKDECDAQVEKALEAGEDP
IDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVAICEDKLGHKSETKAPVMTFAVEGDPHDIGKNLIVMFLNA
NGYGAIDLGRDVPTAEVVKQAQENKPVLMTATALMTTTMTEFSKIVAALQEAGIDTPIGCGGGAVRRDFVEESPQTFYGV
EAYHVPKLADAIVDDGKTWEDIRNEYSDIVGEYVAAYS

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=210, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790450, Length=181, Percent_Identity=27.6243093922652, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535405, Length=225, Percent_Identity=25.7777777777778, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 30300; Mature: 30168

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESPLAKYNDLIFDNYEDIAVRYNVKIEGPALKPEDDPEVVEILPKEEGVKRSLALTVL
CCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHEECCCCCCCCEEEEEEEE
YGDKDECDAQVEKALEAGEDPIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSG
ECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCHHHHHHHHHHHH
VAICEDKLGHKSETKAPVMTFAVEGDPHDIGKNLIVMFLNANGYGAIDLGRDVPTAEVVK
HHHHHHHCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHH
QAQENKPVLMTATALMTTTMTEFSKIVAALQEAGIDTPIGCGGGAVRRDFVEESPQTFYG
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEC
VEAYHVPKLADAIVDDGKTWEDIRNEYSDIVGEYVAAYS
CCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SESPLAKYNDLIFDNYEDIAVRYNVKIEGPALKPEDDPEVVEILPKEEGVKRSLALTVL
CCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHEECCCCCCCCEEEEEEEE
YGDKDECDAQVEKALEAGEDPIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSG
ECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCHHHHHHHHHHHH
VAICEDKLGHKSETKAPVMTFAVEGDPHDIGKNLIVMFLNANGYGAIDLGRDVPTAEVVK
HHHHHHHCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHH
QAQENKPVLMTATALMTTTMTEFSKIVAALQEAGIDTPIGCGGGAVRRDFVEESPQTFYG
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEC
VEAYHVPKLADAIVDDGKTWEDIRNEYSDIVGEYVAAYS
CCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]