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The map label for this gene is thiI

Identifier: 84488989

GI number: 84488989

Start: 200066

End: 201220

Strand: Direct

Name: thiI

Synonym: Msp_0160

Alternate gene names: 84488989

Gene position: 200066-201220 (Clockwise)

Preceding gene: 84488988

Following gene: 84488991

Centisome position: 11.32

GC content: 30.56

Gene sequence:

>1155_bases
ATGGAATACTTTGTAAGATATGGTGAAATTGGAATTAAAAGTCCTAAAATAAGACATAAATTCGAAAAAAGATTAATGGA
TAATATAAAATCAGAACTTGAATGTGAATTTGAAAATAATCAGGGAAGATTAACACTTAAAACAGAAGAAACTAATGAGA
AAGTAGCAGATGTACTCTCAAGAGTGTTTGGAATAGTATCATACAGTCCAATAACACAAACAATAACAGATAAAGAAAAA
ATAAGTGAATTAATTCATGAAATAATACCAAAAGTAATAGATGAAGGAAAATTTAATCCCCAAAAAGATACATTTGCAGT
AAAATGTAGAAGGGTGGGAAATCATGACTTTTCAAGTCAAGAAATGGCAGGATACTGTGGATCTGTAGTTATAGATATAA
CAAATGCAAAAGTAGATCTGTCAAATCCAGACTTTACATTGTATGTGGAAATACGTGAAAATAAAACATACATGTTTACA
GAAAAAATACCTGGACTTGGAGGATTACCTGTAGGATCACAGGGTTCAGTTGTATGTCTTATATCAAGTGGAATAGACTC
ACCAGTTGCTTCATTCCAAATGCTTAAAAGGGGATGTAAAATAATATTACTTCACTGTGATAACAATCCATACACAAAAA
ACACAATAGAAAAGGTTAAAAAACAAGCACAAAGACTACAAAGATACTCTGTAGGAGTAAAAGTTAAATTATACACAATA
AACTTTGGACAATACCTTGAACATGTAATAAAACAAGCACCACCACGTATGACTTGTGTGTTATGTAAGAGTGGGATGTA
TCAAACAGCAGCACAACTTGCTAAATTTAATCATGCAAATGCAATAGCAGATGGAAGTAGTATTGGACAAGTAGCATCAC
AAACCCTAAGAAATATAGAAGCAACACGATATAAATGTAGAATGCCAATATTTAGTCCATTAATAAGTCTTGATAAAATA
GAAATAGAAGCTATAGCTAAAACAATTGGAACATATGAAACATCAATAATTCCTGATGGTGGATGTAGTGCAGTACCAAA
ATATCCAGAAACAAATGCAGAATTAGATGTTGTAAATAATGTAATTGATGATATTAATCAAAAGGATGTTCTTGAAGAAG
TATGGAACACATTTACAAAAATAGATATAGATTGA

Upstream 100 bases:

>100_bases
ACTTCCAGAAGCACCAGGACTACCACCAATATATACAATACTAGCACTATCAAGAAGACTTGGACAATACTTAGCACAAA
AATTATGATGAGGGAAAATA

Downstream 100 bases:

>100_bases
ATATCTATTTTTATACATTTTTTTGGGTGTTAACTTGTAATCTACTTAAAAATATAGATAAAGTACATACAACAGAGTTA
GGAATTAAATATATTAAGAA

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MEYFVRYGEIGIKSPKIRHKFEKRLMDNIKSELECEFENNQGRLTLKTEETNEKVADVLSRVFGIVSYSPITQTITDKEK
ISELIHEIIPKVIDEGKFNPQKDTFAVKCRRVGNHDFSSQEMAGYCGSVVIDITNAKVDLSNPDFTLYVEIRENKTYMFT
EKIPGLGGLPVGSQGSVVCLISSGIDSPVASFQMLKRGCKIILLHCDNNPYTKNTIEKVKKQAQRLQRYSVGVKVKLYTI
NFGQYLEHVIKQAPPRMTCVLCKSGMYQTAAQLAKFNHANAIADGSSIGQVASQTLRNIEATRYKCRMPIFSPLISLDKI
EIEAIAKTIGTYETSIIPDGGCSAVPKYPETNAELDVVNNVIDDINQKDVLEEVWNTFTKIDID

Sequences:

>Translated_384_residues
MEYFVRYGEIGIKSPKIRHKFEKRLMDNIKSELECEFENNQGRLTLKTEETNEKVADVLSRVFGIVSYSPITQTITDKEK
ISELIHEIIPKVIDEGKFNPQKDTFAVKCRRVGNHDFSSQEMAGYCGSVVIDITNAKVDLSNPDFTLYVEIRENKTYMFT
EKIPGLGGLPVGSQGSVVCLISSGIDSPVASFQMLKRGCKIILLHCDNNPYTKNTIEKVKKQAQRLQRYSVGVKVKLYTI
NFGQYLEHVIKQAPPRMTCVLCKSGMYQTAAQLAKFNHANAIADGSSIGQVASQTLRNIEATRYKCRMPIFSPLISLDKI
EIEAIAKTIGTYETSIIPDGGCSAVPKYPETNAELDVVNNVIDDINQKDVLEEVWNTFTKIDID
>Mature_384_residues
MEYFVRYGEIGIKSPKIRHKFEKRLMDNIKSELECEFENNQGRLTLKTEETNEKVADVLSRVFGIVSYSPITQTITDKEK
ISELIHEIIPKVIDEGKFNPQKDTFAVKCRRVGNHDFSSQEMAGYCGSVVIDITNAKVDLSNPDFTLYVEIRENKTYMFT
EKIPGLGGLPVGSQGSVVCLISSGIDSPVASFQMLKRGCKIILLHCDNNPYTKNTIEKVKKQAQRLQRYSVGVKVKLYTI
NFGQYLEHVIKQAPPRMTCVLCKSGMYQTAAQLAKFNHANAIADGSSIGQVASQTLRNIEATRYKCRMPIFSPLISLDKI
EIEAIAKTIGTYETSIIPDGGCSAVPKYPETNAELDVVNNVIDDINQKDVLEEVWNTFTKIDID

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=395, Percent_Identity=27.8481012658228, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 43100; Mature: 43100

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYFVRYGEIGIKSPKIRHKFEKRLMDNIKSELECEFENNQGRLTLKTEETNEKVADVLS
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHHHHHH
RVFGIVSYSPITQTITDKEKISELIHEIIPKVIDEGKFNPQKDTFAVKCRRVGNHDFSSQ
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCH
EMAGYCGSVVIDITNAKVDLSNPDFTLYVEIRENKTYMFTEKIPGLGGLPVGSQGSVVCL
HHHHCCCEEEEEEECCEEECCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEE
ISSGIDSPVASFQMLKRGCKIILLHCDNNPYTKNTIEKVKKQAQRLQRYSVGVKVKLYTI
EECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
NFGQYLEHVIKQAPPRMTCVLCKSGMYQTAAQLAKFNHANAIADGSSIGQVASQTLRNIE
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ATRYKCRMPIFSPLISLDKIEIEAIAKTIGTYETSIIPDGGCSAVPKYPETNAELDVVNN
HHHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
VIDDINQKDVLEEVWNTFTKIDID
HHHCCCHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MEYFVRYGEIGIKSPKIRHKFEKRLMDNIKSELECEFENNQGRLTLKTEETNEKVADVLS
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHHHHHH
RVFGIVSYSPITQTITDKEKISELIHEIIPKVIDEGKFNPQKDTFAVKCRRVGNHDFSSQ
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCH
EMAGYCGSVVIDITNAKVDLSNPDFTLYVEIRENKTYMFTEKIPGLGGLPVGSQGSVVCL
HHHHCCCEEEEEEECCEEECCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEE
ISSGIDSPVASFQMLKRGCKIILLHCDNNPYTKNTIEKVKKQAQRLQRYSVGVKVKLYTI
EECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
NFGQYLEHVIKQAPPRMTCVLCKSGMYQTAAQLAKFNHANAIADGSSIGQVASQTLRNIE
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ATRYKCRMPIFSPLISLDKIEIEAIAKTIGTYETSIIPDGGCSAVPKYPETNAELDVVNN
HHHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
VIDDINQKDVLEEVWNTFTKIDID
HHHCCCHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA