Click here to switch to the map view.

The map label for this gene is mjaIIIR [H]

Identifier: 84488973

GI number: 84488973

Start: 168792

End: 169652

Strand: Direct

Name: mjaIIIR [H]

Synonym: Msp_0144

Alternate gene names: 84488973

Gene position: 168792-169652 (Clockwise)

Preceding gene: 84488972

Following gene: 84488974

Centisome position: 9.55

GC content: 24.74

Gene sequence:

>861_bases
ATGGTTAAATATGAAGTATTAGGTTACACATCACAAGAGGAATATAAGGAAGATTTCTTCAATAATTTATTACCTACAAA
TCATAGTTTTAATTATTTTGTTAATTGGAATAAGGTTTTTAAGAATGTTAAACAATATTCTATTGAAATTTCAATTTTAA
ATAGTTTAAGTAAGGTTGATGAAGAAGATCTTGAAGTAGAATTTAGAAAAATTATCAACTGTTATCCAGAAGTAGTTCCA
TTATTACCTGCTATTTTAGCAATAAGATTTAAATCTAAAAATTTAACTGTGGATATTTTAGAAAAAACATTTAAAACTTA
TAACTTCAATGATGAAGAATTTGATGAAGATGAAATAGTAACTTTTTCTAAAAAAACTGGTTTGTTAAAATTATTTACTA
AAATAAATGATTTATATGCTTATTTGTTGGGAACAGAAGTTGGTTTAGATACTAATGGTAGAAAAAATAGGAGTGGAACA
ATTTTTGAGACATTAATTGAGGAAAATATAAACTCAATATTGGACGGTACAAACTATAAAATCCAATCACAAGGTTATGT
TGATGGTATTTCTCGGAAAAAGAGGGCTGATTTTATAATAACAGATGATGATGAACAAGTTTTAGCTATTGAATGTAATT
ATTATAATGCAACTGGAAGTAAACCAATAGAAGTAACAAATGCGTATATAGAGTTACAAAATGAAATAAGTAATACAAAT
ATAAAATTTTTGTGGATTACTGATGGTCTAGGTTGGAACAAAATGACAAGTACTATTTTAAAGGGGATGGAAAATATTGA
CTTTATTATCAATTACACTATGTTGGAAAATAGTATTAGAAAACTTTTAAATATTGAATAA

Upstream 100 bases:

>100_bases
TTGTATTGATAGAATTCCAGCAAAAAGAAGTATTAATTCAAAAGGAAACAAACGTGGACCAGTAAATGAATTGTTGATAA
AAAATTATTGAGGTTAAACT

Downstream 100 bases:

>100_bases
GTCTCTTTTTTTATCTTATTTTTTTAAATTAATTCTGGCTAATTTTTCATGCTATTTATATATTATTTTTACTATATTAT
AGTATCATTTTATTTTACTT

Product: putative type II restriction-modification system, restriction enzyme

Products: NA

Alternate protein names: R.MjaIII; Endonuclease MjaIII; Type II restriction enzyme MjaIII [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVKYEVLGYTSQEEYKEDFFNNLLPTNHSFNYFVNWNKVFKNVKQYSIEISILNSLSKVDEEDLEVEFRKIINCYPEVVP
LLPAILAIRFKSKNLTVDILEKTFKTYNFNDEEFDEDEIVTFSKKTGLLKLFTKINDLYAYLLGTEVGLDTNGRKNRSGT
IFETLIEENINSILDGTNYKIQSQGYVDGISRKKRADFIITDDDEQVLAIECNYYNATGSKPIEVTNAYIELQNEISNTN
IKFLWITDGLGWNKMTSTILKGMENIDFIINYTMLENSIRKLLNIE

Sequences:

>Translated_286_residues
MVKYEVLGYTSQEEYKEDFFNNLLPTNHSFNYFVNWNKVFKNVKQYSIEISILNSLSKVDEEDLEVEFRKIINCYPEVVP
LLPAILAIRFKSKNLTVDILEKTFKTYNFNDEEFDEDEIVTFSKKTGLLKLFTKINDLYAYLLGTEVGLDTNGRKNRSGT
IFETLIEENINSILDGTNYKIQSQGYVDGISRKKRADFIITDDDEQVLAIECNYYNATGSKPIEVTNAYIELQNEISNTN
IKFLWITDGLGWNKMTSTILKGMENIDFIINYTMLENSIRKLLNIE
>Mature_286_residues
MVKYEVLGYTSQEEYKEDFFNNLLPTNHSFNYFVNWNKVFKNVKQYSIEISILNSLSKVDEEDLEVEFRKIINCYPEVVP
LLPAILAIRFKSKNLTVDILEKTFKTYNFNDEEFDEDEIVTFSKKTGLLKLFTKINDLYAYLLGTEVGLDTNGRKNRSGT
IFETLIEENINSILDGTNYKIQSQGYVDGISRKKRADFIITDDDEQVLAIECNYYNATGSKPIEVTNAYIELQNEISNTN
IKFLWITDGLGWNKMTSTILKGMENIDFIINYTMLENSIRKLLNIE

Specific function: Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme DpnII family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021191
- InterPro:   IPR007637 [H]

Pfam domain/function: PF04556 DpmII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 33229; Mature: 33229

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYEVLGYTSQEEYKEDFFNNLLPTNHSFNYFVNWNKVFKNVKQYSIEISILNSLSKVD
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHEEEEHHHHHHHHCC
EEDLEVEFRKIINCYPEVVPLLPAILAIRFKSKNLTVDILEKTFKTYNFNDEEFDEDEIV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEE
TFSKKTGLLKLFTKINDLYAYLLGTEVGLDTNGRKNRSGTIFETLIEENINSILDGTNYK
EEECCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IQSQGYVDGISRKKRADFIITDDDEQVLAIECNYYNATGSKPIEVTNAYIELQNEISNTN
EECCCCHHCCCCCCCCCEEEECCCCEEEEEEEEEEECCCCCCEEEEHEEEEEECCCCCCC
IKFLWITDGLGWNKMTSTILKGMENIDFIINYTMLENSIRKLLNIE
EEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKYEVLGYTSQEEYKEDFFNNLLPTNHSFNYFVNWNKVFKNVKQYSIEISILNSLSKVD
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHEEEEHHHHHHHHCC
EEDLEVEFRKIINCYPEVVPLLPAILAIRFKSKNLTVDILEKTFKTYNFNDEEFDEDEIV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEE
TFSKKTGLLKLFTKINDLYAYLLGTEVGLDTNGRKNRSGTIFETLIEENINSILDGTNYK
EEECCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IQSQGYVDGISRKKRADFIITDDDEQVLAIECNYYNATGSKPIEVTNAYIELQNEISNTN
EECCCCHHCCCCCCCCCEEEECCCCEEEEEEEEEEECCCCCCEEEEHEEEEEECCCCCCC
IKFLWITDGLGWNKMTSTILKGMENIDFIINYTMLENSIRKLLNIE
EEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]