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The map label for this gene is oprM [H]

Identifier: 83721659

GI number: 83721659

Start: 2575130

End: 2576737

Strand: Reverse

Name: oprM [H]

Synonym: BTH_I2287

Alternate gene names: 83721659

Gene position: 2576737-2575130 (Counterclockwise)

Preceding gene: 83721121

Following gene: 83721139

Centisome position: 67.65

GC content: 75.62

Gene sequence:

>1608_bases
ATGCGCGTTTCGATCCCCCTGCTGCCGCCACGGCTGATTGCCCTGTTGCCGATCGCCGCGCGCGTCGCACCGCGCGCGAG
CGCCACCGCCGCCGCGCTCGCGCTGGCGGGCGCGACGGCCGGCTGCTCGCTGTTCGCGCCGAGCGACGCGCCGCCCGTGA
TGCCGTCGCCCGCGCATTACGGCGCGACGCCGCTGCCCGAGCGCACGGCCGCCGCGCAAGGGCTCGCGCAGCGCTTCGAC
GTCGGCGCGCAGCCGGTGCCCGACTGGTGGAAGCGCTACCGGTCGCCCGCGCTCGACGCGCTCGTCGACGAAGGCTTGCG
CAACAGCCCGACGCTCGCCGCCGCCGAAAAGAACCTGACCGCCGCGCGCGAGCAGTTGCGCGCGCAGATCGGCAGCTCGC
TGCTGCCGTCGATCGACGCGGGCGGCCAGGCGACGCGCGAGCGCGCGCTCGGCGTGCCGGCGTTCGGCCCGCAGACGGTG
CTCTACAACGTGTTCGCGGGCCAGTTGCAGGCGAGCTACACGCTCGACCTGTTCGGCGCCGCGCGCTTCGCGAACCGCGC
GCTCTCGAAGCGCGTCGACATGAACGCGTACCAGCTCGAATCCGCGCGGCGCGCGCTCGCGGCGAACATCGTGACGGCCG
CCCTCACGTCGGCCGCGCTCGCCGAGCGGGTCGCGACGACCGAGCGGCTGGTCGCGCTCGCGCGCGAGCAGGCGAACGAC
GCGGCGGCGCGCCATCGGCTCGGCGCGGCGTCGCGCACCGACATGCTGAACGCGCAGCAAAGCGCGGCGGCGCTCGAGGC
CAGCCTGCCCGCGCTGCGCCAGCAATGGCAGACCACGCGCCACGCGCTCGCCGTGCTGCTCGGCCGCACGCCCGATGCGG
CGCCCGCCGATCTCGCGCTCGCCGACGTGCAACTGCCGCAGGACGTGCCCGTCGTCGTGCCGTCCACGCTGCTGCAGGCG
CGGCCCGACATTCGCGCGGCCGAAGCCGGCTTGCAGGCGGCCGCGTCCGAAGTCGGCGTCGCGACCGCGCAGATGTTCCC
GCAGTTGTCGCTGTCGGCGTCGATGGGTCACGCGGGCTTCAACTGGCCCGCGATGCTGTCGGGCGCGGGCGCGATCTGGA
GCGTCGGCGCATCGCTCACGCAGCCGCTCTTCCACGGCGGCGCGCTGCTCGCGCAACGCCGCGCGGCGCGCGCGACCTAC
GACGCGGCCGTCGACCAGTACAGGCAGACGGTGCTCGCCGCGTTCCAGGACGTCGCCGACAAGCTCGCCGCGCTCGATAA
CGACGCGAGCGCGCTCGACGCGTCGAACCGCTCGGCCGTCGCCGCGCGCGGCGCGTTCGACGACACCGCGGCGCGCGTGC
GGCTCGGCGCGCTGCCGCCGTCGGCCGCGCGCGCGAGCGAGCAGCAGTACCGCAACGCGCGGCTCGACGAGATCCGCGCG
ACGGGCGCGCGGCTCGTCGATACGGCGCGGCTGTTTCAGGCGATGGGTACGCCGACCGACGACGACGCGCACGCGAAGGA
CGCGTCGCGCGCGCGCGACGCAAACCCGCTCGGCAAGGGCGACGCGATCGGCGCGACGCAGGGCCGGATCGAAGCCCGGA
GCCACTGA

Upstream 100 bases:

>100_bases
TCACGCTCTTCTTCCTGCCGGCGCTCTATGCCGCGTGGTTCCGCGTGAAGCCGGACGAGCGCGATCCGGAGTCGCCCGCC
ACGCCTTCGGGAGCCTGACC

Downstream 100 bases:

>100_bases
CGACGCTGCGATCCCGTCCGCCGGCGCGGCGCGCATCGAGCGCATGTTCGCAGTCCCATCCGCTGCGGCCGCGATCGTCA
ACGCCGGCGGAGCCCTACCA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MRVSIPLLPPRLIALLPIAARVAPRASATAAALALAGATAGCSLFAPSDAPPVMPSPAHYGATPLPERTAAAQGLAQRFD
VGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKNLTAAREQLRAQIGSSLLPSIDAGGQATRERALGVPAFGPQTV
LYNVFAGQLQASYTLDLFGAARFANRALSKRVDMNAYQLESARRALAANIVTAALTSAALAERVATTERLVALAREQAND
AAARHRLGAASRTDMLNAQQSAAALEASLPALRQQWQTTRHALAVLLGRTPDAAPADLALADVQLPQDVPVVVPSTLLQA
RPDIRAAEAGLQAAASEVGVATAQMFPQLSLSASMGHAGFNWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARATY
DAAVDQYRQTVLAAFQDVADKLAALDNDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRA
TGARLVDTARLFQAMGTPTDDDAHAKDASRARDANPLGKGDAIGATQGRIEARSH

Sequences:

>Translated_535_residues
MRVSIPLLPPRLIALLPIAARVAPRASATAAALALAGATAGCSLFAPSDAPPVMPSPAHYGATPLPERTAAAQGLAQRFD
VGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKNLTAAREQLRAQIGSSLLPSIDAGGQATRERALGVPAFGPQTV
LYNVFAGQLQASYTLDLFGAARFANRALSKRVDMNAYQLESARRALAANIVTAALTSAALAERVATTERLVALAREQAND
AAARHRLGAASRTDMLNAQQSAAALEASLPALRQQWQTTRHALAVLLGRTPDAAPADLALADVQLPQDVPVVVPSTLLQA
RPDIRAAEAGLQAAASEVGVATAQMFPQLSLSASMGHAGFNWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARATY
DAAVDQYRQTVLAAFQDVADKLAALDNDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRA
TGARLVDTARLFQAMGTPTDDDAHAKDASRARDANPLGKGDAIGATQGRIEARSH
>Mature_535_residues
MRVSIPLLPPRLIALLPIAARVAPRASATAAALALAGATAGCSLFAPSDAPPVMPSPAHYGATPLPERTAAAQGLAQRFD
VGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKNLTAAREQLRAQIGSSLLPSIDAGGQATRERALGVPAFGPQTV
LYNVFAGQLQASYTLDLFGAARFANRALSKRVDMNAYQLESARRALAANIVTAALTSAALAERVATTERLVALAREQAND
AAARHRLGAASRTDMLNAQQSAAALEASLPALRQQWQTTRHALAVLLGRTPDAAPADLALADVQLPQDVPVVVPSTLLQA
RPDIRAAEAGLQAAASEVGVATAQMFPQLSLSASMGHAGFNWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARATY
DAAVDQYRQTVLAAFQDVADKLAALDNDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRA
TGARLVDTARLFQAMGTPTDDDAHAKDASRARDANPLGKGDAIGATQGRIEARSH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=470, Percent_Identity=24.6808510638298, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367348, Length=351, Percent_Identity=26.2108262108262, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55774; Mature: 55774

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSIPLLPPRLIALLPIAARVAPRASATAAALALAGATAGCSLFAPSDAPPVMPSPAHY
CEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
GATPLPERTAAAQGLAQRFDVGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKNLT
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
AAREQLRAQIGSSLLPSIDAGGQATRERALGVPAFGPQTVLYNVFAGQLQASYTLDLFGA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARFANRALSKRVDMNAYQLESARRALAANIVTAALTSAALAERVATTERLVALAREQAND
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAARHRLGAASRTDMLNAQQSAAALEASLPALRQQWQTTRHALAVLLGRTPDAAPADLAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADVQLPQDVPVVVPSTLLQARPDIRAAEAGLQAAASEVGVATAQMFPQLSLSASMGHAGF
ECCCCCCCCCEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARATYDAAVDQYRQTVLAAFQDVAD
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAALDNDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRA
HHHHCCCCHHHHCCCCCCCEEECCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
TGARLVDTARLFQAMGTPTDDDAHAKDASRARDANPLGKGDAIGATQGRIEARSH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVSIPLLPPRLIALLPIAARVAPRASATAAALALAGATAGCSLFAPSDAPPVMPSPAHY
CEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
GATPLPERTAAAQGLAQRFDVGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKNLT
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
AAREQLRAQIGSSLLPSIDAGGQATRERALGVPAFGPQTVLYNVFAGQLQASYTLDLFGA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARFANRALSKRVDMNAYQLESARRALAANIVTAALTSAALAERVATTERLVALAREQAND
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAARHRLGAASRTDMLNAQQSAAALEASLPALRQQWQTTRHALAVLLGRTPDAAPADLAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADVQLPQDVPVVVPSTLLQARPDIRAAEAGLQAAASEVGVATAQMFPQLSLSASMGHAGF
ECCCCCCCCCEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARATYDAAVDQYRQTVLAAFQDVAD
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAALDNDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRA
HHHHCCCCHHHHCCCCCCCEEECCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
TGARLVDTARLFQAMGTPTDDDAHAKDASRARDANPLGKGDAIGATQGRIEARSH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]